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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaatggaggaaaaacatatgacagaacattatgtgccttacacctgtgctcagttcttctggaaaacaaaaacaacttttttcactcaaacata...

Basic information

species Zea mays
transcript GRMZM2G162461_T01
intron # 14
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G162461_T01 (Zea mays), 5'ss of exon 14
lower sequence: LOC_Os02g24430.1 (Oryza sativa), 5'ss of exon 13
ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAA-AAGCAAGgtaatgaaatggaggaaaaacatatgacagaacattatgtgccttacacctgtgctcagttcttctggaaaacaaaaacaacttttttcactcaaacata--------
 |||||||||||||||||||||| ||   ||||||| | ||||||||||||||||| ||||| ||| || | || | ||   ||| |||  | | | ||     ||   |||  |         ||||   || |  |||   ||      |  || || || |    |         
GCCTTGCAGGAGACTCTTGAGCGGCAAGAGGAGATCATGGCATACATTGAGAAGAAAAAGAAGAAGAAAAGCAAGgtaaaaaagggaaattaaaaggcatccttttgc-tgctgtgttgtgaccctcaa--ctacaagaagtaaagttttgcacttgtcgctgaccggtgtaccaaaa

upper sequence: GRMZM2G162461_T01 (Zea mays), 5'ss of exon 14
lower sequence: LOC_Os10g03400.2 (Oryza sativa), 5'ss of exon 13
ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAA-AAGCAAGgtaatgaaatggaggaaaaacatatg-acagaacattatgt-gccttacacctgtgctcagttcttctggaaaacaaaaacaacttttttcactcaaacata---
 |||||||||||||||||||||| ||   ||||||| | ||||||||||||||||| ||||| ||| || | || | ||   |  ||||    | | | |   |||  | | ||  ||    |||  | |    |  |    ||  |      |  || ||||| |  |||    
GCCTTGCAGGAGACTCTTGAGCGACAAGAGGAGATCATGGCATACATTGAGAAGAAAAAGAAGAAGAAAAGCAAGgtaacccaaaaaaagggaaagtaaaaggcatccttttgctgtagtattgttgtgacccttaattactaaacatttttccccttgtcactgacccatgcac
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|14311498|gb|BG901253.1|BG901253
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|8102787|gb|AW927468.1|AW927468
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870569|gb|CF010251.1|CF010251
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|7135744|gb|AW498243.1|AW498243
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCCCGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870385|gb|CF010067.1|CF010067
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAATGAATTGTTCACGCCCCCGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870542|gb|CF010224.1|CF010224
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|6918395|gb|AW399925.1|AW399925
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870512|gb|CF010194.1|CF010194
EST:     AGCGTCAGTTGGAGATCGTTGCATCCATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACACCAGTGAATTGTTCACGCCCCCGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870274|gb|CF009956.1|CF009956
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|8102786|gb|AW927467.1|AW927467
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870585|gb|CF010267.1|CF010267
EST:     GAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: GAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870513|gb|CF010195.1|CF010195
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870386|gb|CF010068.1|CF010068
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870675|gb|CF010357.1|CF010357
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870286|gb|CF009968.1|CF009968
EST:     GTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: GTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|14324095|gb|BG929572.1|BG929572
EST:     TGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: TGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|21390916|gb|BQ528965.1|BQ528965
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870275|gb|CF009957.1|CF009957
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|21390754|gb|BQ528803.1|BQ528803
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|91050815|gb|EB161233.1|EB161233
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|6918678|gb|AW400208.1|AW400208
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|14255699|gb|BG878607.1|BG878607
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32870497|gb|CF010179.1|CF010179
EST:     AGCGTCAGTTGGAGATCGTTGCATCCATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|14311611|gb|BG901366.1|BG901366
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|71422239|gb|DR805663.1|DR805663
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|78091380|gb|DV519754.1|DV519754
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|7137803|gb|AW499278.1|AW499278
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|211223043|gb|FL464247.1|FL464247
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|32869242|gb|CF008924.1|CF008924
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|8382999|gb|BE056047.1|BE056047
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
EST: gi|71422238|gb|DR805662.1|DR805662
EST:     AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAG                         CGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA
genomic: AGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 42 (downstream exon)
Score: 38

ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGAGAAAAAAGGTTTTGCGTGTGATGGAAGCGAGCTGGTGCTTGCGTATGCC


Block sizes: 43 (upstream exon), 37 (downstream exon)
Score: 15

ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGAGAAAAAAGGTTTTGCGTGTGATGGAAGCGAGCTGGTGCTTGCGTATGCC


Block sizes: 28 (upstream exon), 33 (downstream exon)
Score: 2

ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGAGAAAAAAGGTTTTGCGTGTGATGGAAGCGAGCTGGTGCTTGCGTATGCC


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 4134

ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaat ... gttcctgcagCGTTTCTTTCGTTGGTAGAAGCAAACAGCAGTGAATTGTTCACGCCCACGAGAAAAAAGGTTTTGCGTGTGATGGAAGCGAGCTGGTGCTTGCGTATGCC


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ACCTTGCAGGAGACTCTTGAGCGTCAGTTGGAGATCGTTGCATACATTGAGAAGAAGAAGAAAAGCAAGgtaatgaaatggaggaaaaacatatgacagaacattatgtgccttacacctgtgctcagttcttctggaaaacaaaaacaacttttttcactcaaacata

- - - - - - - - - - - - -  TTGGAG
- - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - -  GGAGAT
- - - - - - - - - - - - - - - GAGATC
- - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aggaaaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aaacaaaa