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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtatcctcaaatgattattatctagtttctttttccttcttttacccacctccttgtgatgatggggattataacacaaaacgagatttgtgctttgcag

Basic information

species Zea mays
transcript GRMZM2G000645_T02
intron # 14
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: LOC_Os06g05180.1 (Oryza sativa), 5'ss of exon 14
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtatcctc-aaatgattattatctagtttctttttccttcttttacccacctccttgtgatgatg-----gggattataacacaaaacgagatttgtgctttgcag--------
||  |||| ||||||||||| ||||||||||||||||||||||||||||||||||||||||| || |||||||| ||||| ||||||||  || ||||| | ||||| |  ||||||||  | ||||  |           ||||   |         |  ||  ||  ||   |  |||||  ||||           
AACCTTTATTGGGCTGATAGCGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATACTGAAGTACAACgtaagttattgtattatctaaatgttcattatgttatttcttttctcctcttgaatgtgttattgcgtgaaagttctttttttaccatggcatgaagttatgtttgtaatttgtgttcctacag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: GLYMA15G01680.1 (Glycine max), 5'ss of exon 14
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtat---cctcaaatgattattatctagtttctttttccttcttttac---ccacctccttgtgatgatggggattataacacaaaacgagatttgtgctttgcag------------------------------------------------
||  | ||||||||||| |||||||| |||||  |||| || |||||||||||||||||||| || ||||||||||| ||   ||   |   | |||  |  |  ||||     ||   || | ||     ||   ||||| |||       |     |  | ||        || || | |  |||| ||                                                
AACCTCTACTGGGCTGACAGTGGTGATCTAGTAACAATTGCTAGTGATACATCATTCTACATTCTGAAGTACAATgtgaggattttcgtgaacttcatcttttccttatagttgttggcttgctttgaagaacatcccaccccctccccccaacacacac-acaatttctgctga-atgtatattttgtagatctttactggtagtgcccttgaaacatattgacttccattattgcag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: GLYMA07G03890.2 (Glycine max), 5'ss of exon 3
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtatcctcaaatgattattatctagtttctttttccttct--tttacccacctccttgtgatgatggggattataacacaaaacgagatttgtgctttgcag------
||  | |||||||||||||||||||| |||||  | || || ||||||||||||||||| ||||| ||||||||||| ||       || || || ||        |    |   || |  || |  || |   |    ||  ||    |    | |  | |||   |     |||    |   | |      
AACCTCTACTGGGCTGATAGTGGTGATCTAGTGACGATTGCTAGTGATACATCATTCTATATCCTGAAGTACAATgtgagg------attctaaattgtcccccccccccctctcttatgtttttggttaaatgaatatctgatggcacttactagtgaagcactgtaaacattttaacttccactgttgcag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: GLYMA13G43680.2 (Glycine max), 5'ss of exon 14
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtat---cctcaaatgattattatctagtttctttttccttc-------ttttacccac--ctccttgtgatgatggggattataacacaaaacgag-atttgtgctttgcag------------------------
||  | ||||||||||| |||||||| |||||  |||| || || ||||||||||||||||| || ||||||||||| ||   |   ||   | |||  |  |  ||||  |  ||   || | |||       |||| |||    ||  || ||  ||  |    | | ||| |     |    | ||||  |  |                         
AACCTCTACTGGGCTGACAGTGGTGATCTAGTAACAATTGCTAGCGATACATCATTCTACATTCTGAAGTACAATgtgaggactctcatgaacttcatcttttccttatagatgttggcttgctttcaagaatattttgccccttactatttctgccgaatgtatatttgacagatctttactggtagtgccctttaaacattttgacttccactattgcag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: GLYMA08G22140.1 (Glycine max), 5'ss of exon 14
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaag-atattgtatcctcaaatgatt-attatctagtttctttttccttctt--------ttacccacctccttgtgatgatggggattataacacaaaacgagattt----gtgctttgcag---------------------------
||  | |||||||||||||| |||||  ||||  | || || ||||||||||||||||| ||||| ||||||||||| || ||  |  || ||  |    || ||| |  | ||  |   |  || |         |      || || | || |  |||  |    || |    | |  | ||    |||     |||                           
AACCTGTACTGGGCTGATAGCGGTGATTTAGTGACGATTGCTAGTGATACATCATTCTATATCCTTAAGTACAATgtgaggattctaaatacttgacccttttattttacacttgttgactttttatgaaaaagcatcgttttcccccctatgtttttggttaaatgaatatctgatggcacttactagtgaagcacagtaaacattttaacttccattgttgcag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: AT1G79990.1 (Arabidopsis thaliana), 5'ss of exon 18
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtatcctcaaatgattattatctagtttctttttcc--ttcttttacccacctcctt-gtgatgatggggattataacacaaaacgagatttgtgctttgcag-----------
||| |||| ||||| || |||||||| ||||||||||| || ||||||||||||||||||||||| |||| |||||| ||    | | |       || |  | | | ||     || | |  ||| ||     |  | | | ||  || ||  | |    |||       |   |  |   || | |           
AATCTTTATTGGGCGGACAGTGGTGATCTAGTTGCAATTGCCAGTGATACATCATTCTACATCCTTAAGTTCAATgtgaggatcttttttaaaccttggtagtgagccagaactcttgtacatttccttagtttattttcctagtactgttgctgttcgagacatggttttaagctgttttcttactgttctgctgtag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: AT3G15980.2 (Arabidopsis thaliana), 5'ss of exon 14
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgt--atcctcaaatgattattatctagtttctttttccttcttttacccacctccttgtgatgatggggattataacacaaaacgagatttgtgct-ttgcag
||| |||| |||||||| ||||||||  |||| || || || |||||||| |||||||||||| | ||||||||||| |||   |||  | ||||||||| | |     || |||| ||            ||||| | | ||   || |    |   ||         | | |    ||| || |||
AATCTTTATTGGGCTGAAAGTGGTGATTTAGTAGCCATTGCTAGTGATACGTCATTCTACATCTTGAAGTACAATgtgagaatctgttcaacctcaaatgttcagctattattttcatt------------cccactttcgtggattgttcaaaagcttactctg----gtgttcctcgctattacag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: Vv11s0016g04180.t01 (Vitis vinifera), 5'ss of exon 14
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtatcctcaaatgattattatctagtttctttttccttcttttacccacctccttgtgatgatggggattataacacaaaacgagattt-gtgctttgcag--------------------------
||| |||| |||||||||||||||||  |||| ||||| ||||||||| ||||||| |||||| | |||||||||||||| | || |  | |        | |||||    || | |         | |   |  |       |   |     || ||  |  |   ||||||  || | |  |                            
AATCTTTATTGGGCTGATAGTGGTGATTTAGTGGCAATTGCAAGTGATTCATCATTTTACATCTTAAAGTACAATgtaagct-ttctccctttttggttctcttatcccacttttatgagaaatcatcatagatttgtcataaaatctattcgttttacaatcatttgagattaagtacctcacttaacaaccaaatatgtttatttggcag

upper sequence: GRMZM2G000645_T01 (Zea mays), 5'ss of exon 14
lower sequence: Vv09s0002g04810.t01 (Vitis vinifera), 5'ss of exon 14
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgta-agatattgtatcctcaaatgat--tattatctagtttctttttcctt-cttttacccacct--ccttgtg-atgatggggattataaca---caaaacgagatttgt--------gctttgcag
||  | || |||||||||||||| ||  | || || || || |||||||||||||| |||||||| ||||||||||||  |||  |  |      |||  |  | ||| || || |  || ||||| | | ||   |  |  ||   || |||||  |||  |   ||   ||||  ||  ||| |          |||||||
AACCTCTATTGGGCTGATAGTGGGGATTTGGTGGCCATTGCCAGTGATACATCATTTTACATCCTGAAGTACAATgtatggatcctcaaattattaatcctctttttaacttgtgtaattctcctttcctctatagaattggccaaatgcatgatttggagcactgcattccaaattgaactttttttaatttaattttgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|166586991|gb|EG137729.1|EG137729
EST:     CCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAAT                         AGAGATATTGTTGCTTCTTATCTAGAAGGTGGAAAGCC
genomic: CCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatatt ... tgctttgcagAGAGATATTGTTGCTTCTTATCTAGAAGGTGGAAAGCC
EST: gi|31353243|gb|CD437600.1|CD437600
EST:     ACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAAT                         AGAGATATTGTTGCTTCTTATCTACAAGGTGGAAAGCCTGTGGACGAAGAA
genomic: ACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatatt ... tgctttgcagAGAGATATTGTTGCTTCTTATCTAGAAGGTGGAAAGCCTGTGGACGAAGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 213

AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatatt ... tgctttgcagAGAGATATTGTTGCTTCTTATCTAGAAGGTGGAAAGCCTGTGGACGAAGAAGGCGTTGAAGATGCTTTTGAGCTGCTTCATGAGATCAATGAGCGAGTGA


Block sizes: 46 (upstream exon), 47 (downstream exon)
Score: 57

AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatatt ... tgctttgcagAGAGATATTGTTGCTTCTTATCTAGAAGGTGGAAAGCCTGTGGACGAAGAAGGCGTTGAAGATGCTTTTGAGCTGCTTCATGAGATCAATGAGCGAGTGA


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 55

AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatatt ... tgctttgcagAGAGATATTGTTGCTTCTTATCTAGAAGGTGGAAAGCCTGTGGACGAAGAAGGCGTTGAAGATGCTTTTGAGCTGCTTCATGAGATCAATGAGCGAGTGA


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 154

AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatatt ... tgctttgcagAGAGATATTGTTGCTTCTTATCTAGAAGGTGGAAAGCCTGTGGACGAAGAAGGCGTTGAAGATGCTTTTGAGCTGCTTCATGAGATCAATGAGCGAGTGA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AATGTTTACTGGGCTGATAGTGGTGACCTAGTTGCAATAGCAAGTGATACATCATTCTACATCCTCAAGTACAATgtaagatattgtatcctcaaatgattattatctagtttctttttccttcttttacccacctccttgtgatgatggggattataacacaaaacgagatttgtgctttgcag

- - - - - - - - - - - - - - - - - - - AGCAAG