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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagcttttgagacaacccttactagcaatgtttttgaaaagagtttaagtttttttattgctttgtgcag

Basic information

species Zea mays
transcript GRMZM2G155384_T01
intron # 13
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G155384_T02 (Zea mays), 5'ss of exon 13
lower sequence: LOC_Os02g05340.1 (Oryza sativa), 5'ss of exon 13
TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagcttttgagacaac-ccttactagcaatgttttt-gaaaagagtttaagtttttttat----tgctttgtgcag
||| |||||||| ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| || |||||||| ||||| |||||||||| |  | ||||    |||||   | |  |||  ||  | ||  | | | |    |     |  | |||||||
TTTAATTGATGGGCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAAAATTTAGCTGCAACATTTGTAAATGCATTCGTAAATGCTGGATTTGGTCAGgttagctctacaaacaatatgttacttttagttctttccgaggaaagaatgaatctcaagaccatatcttctgtgcag

upper sequence: GRMZM2G155384_T02 (Zea mays), 5'ss of exon 13
lower sequence: GLYMA06G44340.1 (Glycine max), 5'ss of exon 13
-TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagcttttgagacaacccttactagcaatgtttttgaaaagagtttaagtttttttattgctttgtgcag--------------------------------------
  ||||| |  || | | ||||  |  | ||| ||||||| || ||||||||  | |||||||| ||||| ||||||||||| |||||||| ||||| |||||    | || |   ||   ||        ||| ||||    || |||  |||  |    |  | | | |                                        
GCTTGATGGCCGGGC-TAGTGCTGGTGCAAGTGTTGATTCAGCTAGACAGAACCTTGCTGCAACTTTTGTTAATGCATTTGTAAATGCTGGCTTTGGTCAGgtacaatgtt-atttaaagattctatatgttgtatttgttgtgattttgggttaatgatctcttctattcctcaatcttctacttctcacttcccattatcattgcgcag

upper sequence: GRMZM2G155384_T02 (Zea mays), 5'ss of exon 13
lower sequence: GLYMA12G13410.1 (Glycine max), 5'ss of exon 13
-TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagcttttgagacaacccttactagcaatgtttttgaaaagagtttaagttttt---ttattgctttgtgcag--------------------------------------
  ||||| |  || | | ||||  |  | ||| |||| || || ||||||||  | |||||||| ||||| ||||||||||| |||||||| ||||| |||||    | ||    | |   |||  |    ||| ||||    || |||  |||  |    ||||  | | | |                                        
GCTTGATGGCCGGGC-TAGTGCTGGTGCAAGTGTTGACTCAGCTAGACAGAACCTTGCTGCAACTTTTGTTAATGCATTTGTAAATGCTGGCTTTGGTCAGgtacaatgttatttctaga-ttatgaatgttgtatttgttgtgattttgggttaatgatctattcttctattcctcaatcttctacttctcacttcccattatcattgtgcag

upper sequence: GRMZM2G155384_T02 (Zea mays), 5'ss of exon 13
lower sequence: AT2G20580.1 (Arabidopsis thaliana), 5'ss of exon 11
-TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagcttttgagacaacccttactagcaatgtttttgaaaagagtttaagtttttttatt-gctttgtgcag
  | ||  |  || | | |  || |  | ||  | |||||||| ||||||||||| ||||| || ||||| |||||||| || |||||||| ||||| |||||  | ||       |      | |||  || |  | ||| |    |    ||  | |||| ||||   ||||
ACTCGACGGCAGGGC-TAGCTCCGGTGCAAGCGTGGATTCTGCTAGACAGAATTTGGCTGCTACCTTTGTCAATGCATTCGTAAATGCTGGTTTTGGTCAGgtatggttgcagattataataaaatag--atctagtggaagacgtgtcctcttaatatatttgcttatcgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33093842|gb|CF053836.1|CF053836
EST:     ATTTAGCTGCGACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAG                         GACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA
genomic: ATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA
EST: gi|32934754|gb|CF039566.1|CF039566
EST:     ATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAG                         GACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA
genomic: ATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA
EST: gi|32834497|gb|CD974175.1|CD974175
EST:     ATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAG                         GACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA
genomic: ATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA
EST: gi|32833976|gb|CD973654.1|CD973654
EST:     ATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAG                         GACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA
genomic: ATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 350

TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGAAATTGGTTATTCAAGAACAAGGAACATGGAAAAGCTAGTGCAGCAGCTA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 204

TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGAAATTGGTTATTCAAGAACAAGGAACATGGAAAAGCTAGTGCAGCAGCTA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 161

TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGAAATTGGTTATTCAAGAACAAGGAACATGGAAAAGCTAGTGCAGCAGCTA


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 188

TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagctttt ... ctttgtgcagGACAAGTTGATGACCGCTCCATCTGATTCATCCAGCAGCGGGTCTTCCGGAAATTGGTTATTCAAGAACAAGGAACATGGAAAAGCTAGTGCAGCAGCTA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TTTGATTGATGGTCGTGGTGCCAGCTCCAGTCTTGATTCTGCAAGACAGAATTTAGCTGCAACATTTGTAAATGCATTTGTGAATGCTGGGTTTGGCCAGgttagcttttgagacaacccttactagcaatgtttttgaaaagagtttaagtttttttattgctttgtgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  ATGCTG