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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctgcatgcatacaatatagctatacatctatttacatcttccatgaaagtcatttcaagcatttaattgttctttcaacag

Basic information

species Zea mays
transcript GRMZM2G070045_T01
intron # 13
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G070045_T01 (Zea mays), 5'ss of exon 13
lower sequence: LOC_Os02g51430.2 (Oryza sativa), 5'ss of exon 17
GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctgcatgcatacaatatagctatacatctatt--tacatc--ttccatgaaagtcat--ttcaagcatttaa-ttgttctttcaacag------
|||||||||||||| |||||||||||||||||||||||||||||||||||| || ||| | |||||       | | | || ||  ||| ||  | |||| | | | ||  ||| || ||  || ||  ||    || |       
GTTAATTTTGAAGATACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgttctacat-cttacaagcataagacataactgttactacctccgtcccataataattatatttctaggatacaaatttgtcccaaaataattgtca

upper sequence: GRMZM2G070045_T01 (Zea mays), 5'ss of exon 13
lower sequence: GLYMA03G03120.1 (Glycine max), 5'ss of exon 19
GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctgcatgcatacaatatagctatacatctattta-catct-tccatgaaagtcatttcaagcatttaattgttcttt-caacag----------
|||||||||||||| ||||| || |||||||||||||| || ||||| ||    |  || | |   | |   |  | |  | | |    | | |   ||   ||  |||      |  ||||||||    ||| |           
GTTAATTTTGAAGATACCTGTAAGGGATTTCTTGAGGTAGCTAAGgtatgaaa-gagtgtagaatgttttattcaatacttttctggtgtgtgtgtgtgtgtgttgtttttctactgcaattgttcagcacaatatatgatgcttt

upper sequence: GRMZM2G070045_T01 (Zea mays), 5'ss of exon 13
lower sequence: Vv03s0097g00670.t01 (Vitis vinifera), 5'ss of exon 18
GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctgcatgcatacaatat-agctatacatctatttacatcttccatgaaagtcatttcaagcatttaattgttctttcaacag-----------
||||||||||||||||| |||||||| || || |||||||| ||||| |||      |   | || | | |  |||       ||||| | |    |  |   |||  ||    |||   ||  | | ||               
GTTAATTTTGAAGACACTTGCAAAGGTTTCCTGGAGGTTGCAAAGgtctgttgaaggttatttcatttttatttattttctcttttacttttaaattatatagcatgccatatttttttgtgcacctccagtgtttcttgttgca

upper sequence: GRMZM2G070045_T01 (Zea mays), 5'ss of exon 13
lower sequence: EFJ12004 (Selaginella moellendorffii), 5'ss of exon 17
GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctgcatgcatacaatatagctatacatctatttacatcttccatgaaagtcatttcaagcatttaattgttctttcaacag
|| ||||||||||||||||| ||||| ||||||||||| ||||||||  |    |     ||  |  ||      |   | |  | |||  ||   |                                    
GTCAATTTTGAAGACACCTGTAAAGGGTTTCTTGAGGTGGCCAAGgtgaggagggagatgatgtagaatgaggcgatgacaaagtgcattctcgtag------------------------------------

upper sequence: GRMZM2G070045_T01 (Zea mays), 5'ss of exon 13
lower sequence: EFJ08885 (Selaginella moellendorffii), 5'ss of exon 17
GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctgcatgcatacaatatagctatacatctatttacatcttccatgaaagtcatttcaagcatttaattgttctttcaacag
|| |||||||||||||| || ||||| ||||||||||| ||||||||  |    |     ||  |  ||      |   | |  | |||  ||   |                                    
GTCAATTTTGAAGACACATGTAAAGGGTTTCTTGAGGTGGCCAAGgtgaggagggaggtgatgtagaatgaggcgatgacaaagtgcattctcgtag------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67037440|gb|CO464256.1|CO464256
EST:     GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAG                         GAGGCTGTCCTCCAAACTGTTAGTGTTATTTTTGAAGAACCAGGTGTGCAG
genomic: GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctg ... ctttcaacagGAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAG
EST: gi|67016798|gb|CO445547.1|CO445547
EST:     GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAG                         GAGGCTGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAG
genomic: GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctg ... ctttcaacagGAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAG
EST: gi|24934258|gb|CA452476.1|CA452476
EST:     GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAG                         GAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAG
genomic: GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctg ... ctttcaacagGAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 132

GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctg ... ctttcaacagGAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAGGATCTACTCGTGAAGCTTTACCAGAAAG


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 55

GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctg ... ctttcaacagGAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAGGATCTACTCGTGAAGCTTTACCAGAAAG


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 83

GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctg ... ctttcaacagGAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAGGATCTACTCGTGAAGCTTTACCAGAAAG


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 134

GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctg ... ctttcaacagGAGGCCGTCCTCCAAACTGTTAGTGTTATTTTTGAAGACCCAGGTGTGCAGGATCTACTCGTGAAGCTTTACCAGAAAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTTAATTTTGAAGACACCTGCAAAGGATTTCTTGAGGTTGCCAAGgtttgtgctgcatgcatacaatatagctatacatctatttacatcttccatgaaagtcatttcaagcatttaattgttctttcaacag

- - -  TTGAAG
- - - - TGAAGA
- - - - - - - - -  GCAAAG