ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

ATGGGAGAGGTAAAAGACCAAAGAATGCACCCCCTACTGCTCGCTCCCAAGGTACCAGGGCGGTTTTACTTCGTCTTTGGGAAGCCTATCGAAACAAGAGgtacgggttcagaaacatttcgttacatcatatctcagcagtctgtgctcttcaggcgttctctcggtgtcagcttttatgttggataccgacgcatgcgcatgcgtttgaccgtttctcgaacctggtgttgggttgaaaacaaatcattcgctttgcttgcttgcaatag

Basic information

species	Zea mays
transcript	GRMZM5G817559_T01
intron #	12
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM5G817559_T01 (Zea mays), 5'ss of exon 12
lower sequence: LOC_Os09g33530.1 (Oryza sativa), 5'ss of exon 13

ATGGGAGAGGTAAAAGACCAAAGAATGCACCCCCTACTGCTCGCTCCCAAGGTACCAGGGCGGTTTTACTTCGTCTTTGGGAAGCCTATCGAAACAAGAGgtacgggttcagaaacatttcgttacatcatatctcagcagtctgtgctcttcaggcgttctctcggtgtcagcttt-tatgttggataccgacgcatgcgcatgcgtttgaccgtt--tctcgaacctggtgttgggttgaaaa--------caaatcattcgctttgcttgcttgcaatag
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ACCGGAGAATTAAAATATCAAAGAATCCATCCAGTAGTGGCTGCCCCAAAGATACCAGGGAGGTTTTATTTCATCTTTGGGAAGCCAATTGAAACAAGAGgtacatctctaaaga-actacgcaagtaaatctccaattaatatacctgtcgcacgcactgtctcatgacaagttaaacacgcttactttcttttggcaatctgaagataagctactaggctagaacaca-tgaagtgatgaagatgtttttccgagacctgatttttgcttgttttca-tag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|8665961|gb|BE186777.1|BE186777

EST:     GCCTATCGAAACAAGAG                         GCAGAGAGAAAGAACTACGAGACAAGGAGGCGGCGCAGCGCCTCTATTTGC
genomic: GCCTATCGAAACAAGAGgtacgggttc ... cttgcaatagGCAGAGAGAAAGAACTACGAGACAAGGAGGCGGCGCAGCGCCTCTATTTGC

EST: gi|71441753|gb|DR822803.1|DR822803

EST:     GGTACCAGGGCGGTTTTACTTCGTCTTTGGGAAGCCTATCGAAACAAGAG                         GCAGAGAGAAAGAACTACGAGACAAGGAGGCGGCGCAGCGCCTCTATTTGC
genomic: GGTACCAGGGCGGTTTTACTTCGTCTTTGGGAAGCCTATCGAAACAAGAGgtacgggttc ... cttgcaatagGCAGAGAGAAAGAACTACGAGACAAGGAGGCGGCGCAGCGCCTCTATTTGC

EST: gi|211210005|gb|FL182890.1|FL182890

EST:     GGTGCCAGGGCGGTTTTACTTCGTCTTTGGGAAGCCTATCGAAACAAGAG                         GCAGAGAGAAAGAACTACGAGACAAGGAGGCGGCGCA-CGCTCTTTATTTG
genomic: GGTACCAGGGCGGTTTTACTTCGTCTTTGGGAAGCCTATCGAAACAAGAGgtacgggttc ... cttgcaatagGCAGAGAGAAAGAACTACGAGACAAGGAGGCGGCGCAGCGC-CTCTATTTG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 44 (upstream exon), 45 (downstream exon)
Score: 20

ATGGGAGAGGTAAAAGACCAAAGAATGCACCCCCTACTGCTCGCTCCCAAGGTACCAGGGCGGTTTTACTTCGTCTTTGGGAAGCCTATCGAAACAAGAGgtacgggttc ... cttgcaatagGCAGAGAGAAAGAACTACGAGACAAGGAGGCGGCGCAGCGCCTCTATTTGCAAGTCAAATCTGAGGTGGAGGGCTGCATCAACTATCTCAAGGAGAAGAG

Block sizes: 48 (upstream exon), 33 (downstream exon)
Score: 68

Block sizes: 47 (upstream exon), 40 (downstream exon)
Score: 118