ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TTCCTTGAGATTCATCTCTTCTGGGCTGGCAAGGGCACTTGTTGCATTCCTTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAAgtactcatctgaactttaacttgttctacattgcagttatactactgcccaagtgacattttgaagtattaatcaatgcatactagtgtagtcacaaaaa...

Basic information

species	Zea mays
transcript	GRMZM2G337532_T01
intron #	12
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G337532_T01 (Zea mays), 5'ss of exon 12
lower sequence: LOC_Os04g52590.1 (Oryza sativa), 5'ss of exon 14

TTCCTTGAGATTCATCTCTTCTGGGCTGGCAAGGGCACTTGTTGCATTCCTTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAAgtactcatctgaactttaactt------gttctacattgcagttatacta-ctgccca-agtgacattttgaagtattaatcaatgcatactagtgtagtcacaaaaa
||||||||||||||||||||||||||||||||||| ||||| ||||||||| || | || |||||||| ||||||||||||||  |||| || |||||||||||| |||| ||||  || ||      |||||| ||| | ||||||||| |||  || |  | ||||| | |||   |   ||   |||   || ||          
TTCCTTGAGATTCATCTCTTCTGGGCTGGCAAGGGTACTTGCTGCATTCCTCATCAAGGCTACTATGGGCCTGCAATATCAGCTTTGAGCGCTACTCCAAgtactgatctaaactagaattttttttcgttctatatttctgttatactaactgttcacaacgccatttgggagt--catctaagatatatcggtatacctc------

upper sequence: GRMZM2G337532_T01 (Zea mays), 5'ss of exon 12
lower sequence: GLYMA08G25590.1 (Glycine max), 5'ss of exon 20

TTCCTTGAGATTCATCTCTTCTGGGCTGGCAAGGGCACTTGTTGCATTCCTTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAAgtactcatctgaa------ctttaacttg--ttctacattgcagttatactactgcccaagtgacattttgaagtat-taatcaa----tgcatactagtgtagtcacaaaaa------------------
|  ||||||||||||||||| |||||||| || || ||||||||||| ||   | | ||    ||||| ||   |||   |||  |   ||| | |||| ||| |  | ||||         ||| ||   |  |  ||| |     |   | |   ||     | || | |  ||  || ||||    |  ||| |  | || |   | | |                  
TATCTTGAGATTCATCTCTTTTGGGCTGGAAAAGGTACTTGTTGCATACCAACTCAAGGTACTTATGGGCCCTTAATTCAAGCAATCCATGCTATTCCAGgtaatactatgaaaaaaattggtaatttagatgtttaattactaacctttcattatgcataatcccttatcagctagatattcaaaatttatatatttttttaatttgatacattgttttgtgttgtacag

upper sequence: GRMZM2G337532_T01 (Zea mays), 5'ss of exon 12
lower sequence: GLYMA08G25600.1 (Glycine max), 5'ss of exon 17

TTCCTTGAGATTCATCTCTTCTGGGCTGGCAAGGGCACTTGTTGCATTCCTTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAAgtactcatctgaactttaacttgttctacattgcagttatactactgcccaagtgacattttgaagtattaatcaa-tgcatactagtgtagtcacaaaaa----------------------------------
|  ||||||||||||||||| |||||||| || || ||||||||||| ||   | | ||    ||||| ||   |||   |||  |   ||| |  ||| ||| |  | ||||    || ||| |  |  | |  |||  | ||||  ||       ||    ||    | ||||  |  ||| |     | | | | | |                                  
TATCTTGAGATTCATCTCTTTTGGGCTGGAAAAGGTACTTGTTGCATACCAACTCAAGGTACTTATGGGCCCTTAATTCAAGCAATCCATGCTATCCCAGgtaatactatgaaaaa-aatttggta-atttagatgtttaattactaacctttcattatgcataatcccttatcagctagatattca-aaatttatatatatttttaatttgataaattgttttgtgttgtacag

upper sequence: GRMZM2G337532_T01 (Zea mays), 5'ss of exon 12
lower sequence: Vv00s0125g00210.t01 (Vitis vinifera), 5'ss of exon 2

TTCCTTGAGATTCATCTCTTCTGGGCTGGCAAGGGCACTTGTTGCATTCCTTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAAgta-ctcatctgaactttaactt---gttctacattgcagttatactactgcccaagtgacattttg--aagtattaatcaat-gcatactagtgtagtcacaaaaa
|  ||||| |||||||| ||||||||||| ||||| ||||| ||||| |||||| | || || ||||| ||  | || |||||||| ||||    | || ||| | ||| | ||   ||  |      |  | ||   |       | ||| |         |||||  || |   | | ||| |  | ||  | || |      | 
TATCTTGAAATTCATCTGTTCTGGGCTGGTAAGGGAACTTGCTGCATACCTTATGATGGTTATTATGGACCATCCATTTCAGCCCTCAGTGTTGTTTCAGgtaacccatttaaatgatagttatagaattcatataatataagatgtcctgactggtgcttcttttgccaactgcaactgaattggttgctgatttattttgctcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|160481411|gb|EY254305.1|EY254305

EST:     TTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAA                         ATTTCGTTCCTACAGTCCGTAGTTCTGAAGACAGCAAGAGCAGCCATAAAA
genomic: TTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAAgtactcatct ... tcttcttcagATTTCGTTCCTACAGTCCGTAGTTCTGAAGACAGCAAGAGCAGCCATAAAA

EST: gi|32837254|gb|CD976932.1|CD976932

EST:     TCAGCCCTGAGTGCAACTCCAA                         ATTTCGTTCCTACAGTCCGTAGTTCTGAAGACAGCAAGAGCAGCCATAAAA
genomic: TCAGCCCTGAGTGCAACTCCAAgtactcatct ... tcttcttcagATTTCGTTCCTACAGTCCGTAGTTCTGAAGACAGCAAGAGCAGCCATAAAA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 68

TTCCTTGAGATTCATCTCTTCTGGGCTGGCAAGGGCACTTGTTGCATTCCTTATAAGGGATACTATGGTCCTGCAATATCAGCCCTGAGTGCAACTCCAAgtactcatct ... tcttcttcagATTTCGTTCCTACAGTCCGTAGTTCTGAAGACAGCAAGAGCAGCCATAAAACTGGTGTGATCGCTGGGGTTGCAGTTGGTGTTTCAGTTTTTGCATTGAT

Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 82

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 153