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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GACTGAAGCCGCTGCTGTGCCTGGCTTAAGGGCTGAATTGGAAGCTCTGAAACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcccattctacacatcataagcttcgattgtgttaatggcttcatcataagctatacaatacacccatggtctaacagctgggtttctgtttt...

Basic information

species Zea mays
transcript GRMZM2G150257_T01
intron # 12
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G150257_T01 (Zea mays), 5'ss of exon 12
lower sequence: LOC_Os05g48620.1 (Oryza sativa), 5'ss of exon 17
GACTGAAGCCGCTGCTGTGCCTGGCTTAAGGGCTGAATTGGAAGCTCTGAAACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcccattctacacatcataagcttcgattgtgttaatggcttcatcataagctatacaatacacccatggtctaacagctgggtttctgtttt------
 || || ||  ||||| ||||||| |||||||||||  |||||||| |||||||||| ||||| |||||||||||  ||||||||||||||||||||||||||  | | || |   |  || ||| |  ||  |||   |||     ||    ||    |||| || | |||  |  || |  |  ||| ||| | |         
TACCGAGGCTTCTGCTCTGCCTGGTTTAAGGGCTGAGCTGGAAGCTTTGAAACAAAGACACTTTCAAGCTCTAGAACTAATGGGGGAACGTGATGAAGAGgtacggcttccctagaaggcaccatgactttatatttgatta-----ttttctatggtatataaaagaaacctttt-tccatgaattggatttatatggagctcgt
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211457220|gb|FK982202.1|FK982202
EST:     AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
EST: gi|14244349|gb|BG842317.2|BG842317
EST:     AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
EST: gi|261490390|gb|GO359881.1|GO359881
EST:     AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
EST: gi|211457221|gb|FK982203.1|FK982203
EST:     AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
EST: gi|116821521|gb|EC864841.1|EC864841
EST:     AACAAAGGCACTTCCAAGCTCTAGAACTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGCAAATGATATT
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAG-AAATGATATT
EST: gi|60341571|gb|DN208544.1|DN208544
EST:     AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
EST: gi|33093995|gb|CF053989.1|CF053989
EST:     AACAAAGGCACTTCCAAGCTCTAGAACTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTAC
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTAC
EST: gi|60340800|gb|DN207773.1|DN207773
EST:     AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAG                         TTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG
genomic: AACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 182

GACTGAAGCCGCTGCTGTGCCTGGCTTAAGGGCTGAATTGGAAGCTCTGAAACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAGCAGGTGGATCTTCTTGTTAGCCAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 109

GACTGAAGCCGCTGCTGTGCCTGGCTTAAGGGCTGAATTGGAAGCTCTGAAACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAGCAGGTGGATCTTCTTGTTAGCCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 193

GACTGAAGCCGCTGCTGTGCCTGGCTTAAGGGCTGAATTGGAAGCTCTGAAACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAGCAGGTGGATCTTCTTGTTAGCCAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 144

GACTGAAGCCGCTGCTGTGCCTGGCTTAAGGGCTGAATTGGAAGCTCTGAAACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcc ... tgttttgcagTTGGAGGAGCTAAGAAATGATATTGTTGACCTAAAAGAGATGTACAGGGAGCAGGTGGATCTTCTTGTTAGCCAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GACTGAAGCCGCTGCTGTGCCTGGCTTAAGGGCTGAATTGGAAGCTCTGAAACAAAGGCACTTCCAAGCTCTAGAGTTAATGGGGGAACGTGATGAAGAGgtattgttcccattctacacatcataagcttcgattgtgttaatggcttcatcataagctatacaatacacccatggtctaacagctgggtttctgtttt

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA