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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CAACATTTTTCGCGTGTTTGGTTGCCGTTCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctacttttcaaagaaaatgtttgaagcaaaactattcaacttctaagcatgttattgtttcttcatgtaaattgatttccatacaccaagctgttttgagtagcatacatgagcacttgtcgctcaaatgtagtaatatggaactaatcgtatctgtattatttactttttcttgtgaacag

Basic information

species Zea mays
transcript GRMZM2G142334_T02
intron # 12
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G142334_T02 (Zea mays), 5'ss of exon 12
lower sequence: LOC_Os01g41630.1 (Oryza sativa), 5'ss of exon 12
CAACATTTTTCGCGTGTTTGGTTGCCGTTCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctacttttcaaagaaaatgtttgaagcaaaactattcaacttctaagcatgttatt-gtttcttcatgtaaattgatttccatacaccaagctgttttgagtagcatacatgagcacttgtcgctcaaatgtagtaatatggaactaatcgtatctgtattatttactttttcttgtgaacag
|||||||||||| |||||||||||  |  ||||||||| ||| ||||| |||||||||||||| ||||||||||| ||||||| || ||  |  | |||  |  | ||||| || || |  |   |  |    || | ||  ||||| | ||||||   |  |  |   |    | |  | |||                                                                                   
CAACATTTTTCGTGTGTTTGGTTGTGGCACTGGTAGCAATGAGGCAACGACACTTGAAGCAACTCGAAACCCTACTAGgtaatccttctcctatatgaa--taatggaagctaa-ctttatactgtacacatctgctgtttatttctgcctgtaaaactaactata---atagtgttcatctga-----------------------------------------------------------------------------------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|120481038|gb|EH211133.1|EH211133
EST:     TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAG                         GCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
genomic: TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
EST: gi|32826308|gb|CD965986.1|CD965986
EST:     TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAG                         GCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATC
genomic: TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATC
EST: gi|67016408|gb|CO445157.1|CO445157
EST:     TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAG                         GCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
genomic: TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
EST: gi|71417754|gb|DR804198.1|DR804198
EST:     TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAG                         GCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCTTAAGTACTCTGAC
genomic: TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
EST: gi|211530906|gb|FL193982.1|FL193982
EST:     TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAG                         GCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
genomic: TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
EST: gi|6012734|gb|AW062171.1|AW062171
EST:     CCGAAACCCTACAAG                         GCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
genomic: CCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
EST: gi|211530907|gb|FL193983.1|FL193983
EST:     TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAG                         GCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC
genomic: TCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 90

CAACATTTTTCGCGTGTTTGGTTGCCGTTCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGACAAGGGCCGTTAGACGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 66

CAACATTTTTCGCGTGTTTGGTTGCCGTTCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGACAAGGGCCGTTAGACGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 99

CAACATTTTTCGCGTGTTTGGTTGCCGTTCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGACAAGGGCCGTTAGACGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 89

CAACATTTTTCGCGTGTTTGGTTGCCGTTCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctac ... ttgtgaacagGCGGCAGGTCCAGACTACCTCTAGAGCTACCAGATCCCTAAGTACTCTGACAAGGGCCGTTAGACGAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CAACATTTTTCGCGTGTTTGGTTGCCGTTCTGGTAGCAGTGAAGCAACAACACTTGAAGCAACCCGAAACCCTACAAGgtaatgctacttttcaaagaaaatgtttgaagcaaaactattcaacttctaagcatgttattgtttcttcatgtaaattgatttccatacaccaagctgttttgagtagcatacatgagcacttgtcgctcaaatgtagtaatatggaactaatcgtatctgtattatttactttttcttgtgaacag

- - - - - - - - - - - - - - - - - - -  TGAAGC
- - - - - - - - - - - - - - - - - - - -  AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCAA