ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GAGGCCTGTATGCACCCTTTCTTCGATGAGCTGAGAGACCCAAACACTCGTCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAGgtagcataattgtactgagacatcagtgttgctattctgtcagtaaatcttctgttaccttaacatgggcatacttgagaatttaacctagctagtttcaaattggattctcagctaaaactgatcagatgtttgcaatactgacag

Basic information

species	Zea mays
transcript	GRMZM2G138676_T05
intron #	11
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G138676_T05 (Zea mays), 5'ss of exon 11
lower sequence: LOC_Os03g62500.1 (Oryza sativa), 5'ss of exon 12

GAGGCCTGTATGCACCCTTTCTTCGATGAGCTGAGAGACCCAAACACTCGTCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAGgtagcataattgtactgagacatcagtgttgctattctgtcagtaaatcttctgttaccttaacatgggcatacttgagaatttaacctagctagtttcaaattggattctcagctaaaactgatcagatgtttgcaatactgacag
|| ||||| |||||||||||||| |||||| ||||||| ||||||||||||||||| ||||| || ||||| || |||||||||||||||||| | ||||||| ||||  || | ||| ||| |  |      ||     |  ||   | | || |   || |         || | |  |||   ||||  |   |     | |                                          
GAAGCCTGCATGCACCCTTTCTTTGATGAGTTGAGAGATCCAAACACTCGTCTACCTAATGGGCGTCCTCTTCCTCCTCTCTTCAACTTCAGAACACAAGgtatcatagctgaa-tgaaacagcgataacagcatatgaaccttag--catttgctgatttgattctcatcaacctaaacatt---cctaaatctctctgcctcgt-----------------------------------------

upper sequence: GRMZM2G138676_T05 (Zea mays), 5'ss of exon 11
lower sequence: AT1G57870.1 (Arabidopsis thaliana), 5'ss of exon 13

GAGGCCTGTATGCACCCTTTCTTCGATGAGCTGAGAGACCCAAACACTCGTCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAGgtagcataattgtactgagacatcagtgttgctattctgtcagtaaatcttctgttaccttaacatgggcatacttgagaatttaacctagctagtttcaaattggattctcagctaaaactgatcagatgtttgcaatactgacag
|| || ||||| ||||||||||| |||||||| |||||||||||  ||||||| || || || ||||| || || || || ||||| || | | | |||||||        || |||  |  |   | ||  | ||       |||||  | | | |    |  |      ||  ||  |  || |   |||  || ||  |  |                                          
GAAGCTTGTATCCACCCTTTCTTTGATGAGCTAAGAGACCCAAATGCTCGTCTGCCAAACGGCCGGCCACTTCCTCCGCTATTCAATTTTAAACCACAAGgtaag------gttctgttatttgcttcttcttttttccgtgttaaattctttataagggaagaaaattagtatgtgtta--ttgata-agcgtgtgtcctacag------------------------------------------

upper sequence: GRMZM2G138676_T05 (Zea mays), 5'ss of exon 11
lower sequence: AT1G09840.1 (Arabidopsis thaliana), 5'ss of exon 12

GAGGCCTGTATGCACCCTTTCTTCGATGAGCTGAGAGACCCAAACACTCGTCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAGgtagcataattgtactgagacatcagtgttgctattctgtcagtaaatcttctgt-taccttaacatgggcatacttgagaatttaacctagctagtttcaaattggattctcagctaaaactgatcagatgtttgcaatactgacag
|| || ||||| || || || || |||||||| || ||||||||||||||||| |||||||| || || || || || || |||||||| | | | |||||||   |     || |      |   |||||     || | |  | ||||    | |    ||   |  |   | |||   |  |          |||    |||||                                         
GAAGCTTGTATCCATCCGTTATTTGATGAGCTAAGGGACCCAAACACTCGTCTTCCCAATGGCCGACCACTTCCTCCACTTTTCAACTTTAAACCGCAAGgtacagttcccttattt-----ttcttgttg--ggcctattaagagatctaacatatgtagtatgctccgttaagttgttgacatgttgcgttgtgttcatgattgg-----------------------------------------

upper sequence: GRMZM2G138676_T05 (Zea mays), 5'ss of exon 11
lower sequence: Vv12s0035g01060.t01 (Vitis vinifera), 5'ss of exon 11

GAGGCCTGTATGCACCCTTTCTTCGATGAGCTGAGAGACCCAAACACTCGTCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAGgtagcataattgtactgagacatcagtgttgctattctgtcagtaaatcttctgtta-ccttaacatgggcatacttgagaatttaacctagctagtttcaaattggattctcagctaaaactgatcagatgtttgcaatactgacag
|| || || || ||||||||||| |||||| |||| ||||| | ||||||||| || ||||| || ||||| || || ||||| || |||| | |||||||||   |   | |  |  |  |||    ||       | | || ||   |  | ||  |||| |  ||   ||  ||   |    ||  |||  | |||   |                                             
GAAGCTTGCATCCACCCTTTCTTTGATGAGTTGAGGGACCCTAGCACTCGTCTTCCTAATGGCCGTCCTCTTCCTCCCCTCTTTAATTTCAAACCTCAAGgtaagtttgatctgatctg-tatccaccttatctacttttgaggaacaataatattgtcctttaggtgatgatgattttcag--aaatttagtaattttatga---------------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|38229772|gb|CF920085.1|CF920085

EST:     TCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAG                         AGCTAAAAGGNGTTCCCCCAGAAGTCATCGATCGCCTTGTCCCA
genomic: TCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAGgtagcataat ... atactgacagAGCTAAAAGGCGTTCCCCCAGAAGTCATCGATCGCCTTGTCCCA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 44 (upstream exon), 46 (downstream exon)
Score: 68

GAGGCCTGTATGCACCCTTTCTTCGATGAGCTGAGAGACCCAAACACTCGTCTACCCAATGGTCGGCCTCTGCCGCCTCTCTTCAACTTCAGATCTCAAGgtagcataat ... atactgacagAGCTAAAAGGCGTTCCCCCAGAAGTCATCGATCGCCTTGTCCCAGAGCACTCAAGAAGGCAGAGTTTGTTCATGGCGCTAAGGACCTAG

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 87

Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 126