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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatgccgtctctgcttctggttgctccgtttgcacttggcagtatgattatccttggcagcagggagcggcgatgtttgaattatgcatttttttctgttgagcctagatcatgactgtgctgggctcgttcaattccactgtgtgcag

Basic information

species Zea mays
transcript GRMZM2G126453_T02
intron # 11
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G126453_T01 (Zea mays), 5'ss of exon 21
lower sequence: LOC_Os11g43970.1 (Oryza sativa), 5'ss of exon 26
AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatgcc-gt-ctctgcttctggttg----ctccgtttgcacttggcagtatgattatccttggcagcag--ggagcggcgatgtttgaatta----tgcatttttttctgttgagcctagatcatgactgtgctgggctcg--ttcaattccactgtgtgcag
||||| || |||||||| || ||||||||||| |||||||| || |||||||||||||||||   || |||  || |  ||||      ||    |   | |     |||    |  | ||     || |   ||  | |      ||  ||  | ||    |  | | ||   ||   |  ||   |  |   | ||||    | |  |||  ||||  | | | |||
AATCTATGCGTGACCGCAGCTCATTACCCCATTCGAGAAATCCTTGAGAAGGAAAAGAAGgttggcattgcttgtaccttgctgtcaactgaagacctagataaagtttgttttttgggtttatgctgtctttagatgcactccttattcttctactacaattatactgttaagtgacaacgctttttattttatatgctaatatggggttcgcttcctttctattcag

upper sequence: GRMZM2G126453_T01 (Zea mays), 5'ss of exon 21
lower sequence: AT4G02480.1 (Arabidopsis thaliana), 5'ss of exon 25
AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatgccgtctctgcttctggttgctccgtttgcacttggcagtatgattatccttggcagcagggagcggcgatgtttgaattatgcatttttttctgttgagcctagatcatgactgtgctgggctcgttcaattccactgtgtgcag
||||||||||||||||| || ||||  || ||||||||||| |||||||| |||||||||||      | | || |     |  | ||||| |  | | |    | |||    ||| | ||    |||     |     | |||  ||||| |    | || | ||||    ||                                         
AATCTGTGTGTGACCGCAGCACATTTTCCAATCCGAGAAATACTGGAGAAAGAAAAGAAGgtttg-gtttctgttta---atgcgattgcttc--tcgaatcctgaagtccattta-cattactcgcataactc---attctttttattattctggctgttgttttgactacag-----------------------------------------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32921157|gb|CF025969.1|CF025969
EST:     TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAG                         GAGAAGAGTTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
genomic: TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
EST: gi|32920203|gb|CF025015.1|CF025015
EST:     TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAG                         GAGAAGAGTTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
genomic: TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
EST: gi|211048841|gb|FL187601.1|FL187601
EST:     TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAG                         GAGAAGAGTTTGGCTAAAACAGAAGG
genomic: TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGG
EST: gi|211048838|gb|FL187598.1|FL187598
EST:     TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAG                         GAGAAGAGTTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
genomic: TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
EST: gi|32921345|gb|CF026157.1|CF026157
EST:     TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAG                         GAGAAGAGTTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
genomic: TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
EST: gi|211048837|gb|FL187597.1|FL187597
EST:     TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAG                         GAGAAGAGTTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
genomic: TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
EST: gi|32920665|gb|CF025477.1|CF025477
EST:     TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAG                         GAGAAGAGTTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA
genomic: TGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 241

AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGAAGCGAGGACATCCGCCCTCTTAGCATAGATGACTTCAAATCTGCCCATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 188

AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGAAGCGAGGACATCCGCCCTCTTAGCATAGATGACTTCAAATCTGCCCATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 249

AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGAAGCGAGGACATCCGCCCTCTTAGCATAGATGACTTCAAATCTGCCCATG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 222

AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatg ... ctgtgtgcagGAGAAGAATTTGGCTAAAACAGAAGGTAGGCCGGAGCCTGCATTATATGGAAGCGAGGACATCCGCCCTCTTAGCATAGATGACTTCAAATCTGCCCATG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AATCTGTGTGTGACCGCGGCGCATTACCCCATCCGAGAAATTCTGGAGAAGGAAAAGAAGgtacacaatgccgtctctgcttctggttgctccgtttgcacttggcagtatgattatccttggcagcagggagcggcgatgtttgaattatgcatttttttctgttgagcctagatcatgactgtgctgggctcgttcaattccactgtgtgcag

- - - - - - - - - - - - - - - - - - - - -  TGGAGA
- - - - - - - - - - - - - - - - - - - - - - GGAGAA
- - - - - - - - - - - - - - - - - - - - - - - - AAGGAA
- - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG