ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TCCTGCCGGTATAAATGCTCTATTTAGTCATGGACCGAGGCGGTCAGCAGGCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGTTgtaagtattcttcacccacctacatttcaatgttatatcggtatctatcacacgtaatggctgcaattgaatgagttttttttttctatcaagtgttgcgcctctataaccttcctgtgcttgttaatatctag

Basic information

species	Zea mays
transcript	GRMZM2G412234_T01
intron #	10
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G412234_T01 (Zea mays), 5'ss of exon 10
lower sequence: LOC_Os01g09246.1 (Oryza sativa), 5'ss of exon 20

TCCTGCCGGTATAAATGCTCTATTTAGTCATGGACCGAGGCGGTCAGCAGGCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGTTgtaagtattcttcacccacctacatttcaatgtta------tatcggtatctatcacacgtaatggctgcaat-tgaatg-agttttttttttctatca------agtgttgcgcctctataaccttcctgtgcttgttaatatctag---------------------------
|||||| || ||||||||||| ||||||||||||||  |||| ||||||||||||||||||||||||||||| |||||||| |||||||||||||||||||||||||| ||    ||| || ||||||| ||||       |||  || | |||   |  |   |     ||   |  || ||||  | | | | || |       || ||     ||    |  ||  | |  ||||| || | |                             
TCCTGCTGGAATAAATGCTCTTTTTAGTCATGGACCACGGCGATCAGCAGGCCTTGCTCGTGTGTATGCATTATGGAACATTACTTCACTGGTCAATGTTgtaagtatgctatgtccatctgcatttcattgttgcatgcttatttgtctatattgtattttgagatcctaagacggctgtagttagtgtctgccattattcactggttttagctttccccgattttgttcttattgttgatttatcttcataatcctcttcccatgatattcag

upper sequence: GRMZM2G412234_T01 (Zea mays), 5'ss of exon 10
lower sequence: GLYMA04G09230.1 (Glycine max), 5'ss of exon 21

TCCTGCCGGTATAAATGCTCTATTTAGTCATGGACCGAGGCGGTCAGCAGGCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGTTgtaagtattcttcacccacctacatttcaatgttatatcggtatctatcacacgtaatggctg-caattgaatgagttttttttttctatcaagtgt--tgcgcctctataaccttcctgtgcttgttaatatctag------------------------
||||| ||| || |||||||| |||||||||||||| ||||| || ||||||||||| ||| | |||||  |||||||  | || ||  |  | |||||||| ||  || |  |   |  | ||  | | |   ||||  | |||  |||       ||  || ||     |||    |||   ||       |||   |       |||    |   | |   || |  || || |                        
TCCTGTCGGAATTAATGCTCTCTTTAGTCATGGACCAAGGCGTTCTGCAGGCCTTGCACGTCTTTATGCTCTGTGGAATCTTACATCCTTCATAAATGTTgtgag--ttatctattgaatttcagctgattaccatatttg-atccttcaatttatttgtttgtcaggctgatggacctttccatttatcagggtggaatatagtaatatggattatatttttctggttttaactggggatctcgtgtcatttgtcactag

upper sequence: GRMZM2G412234_T01 (Zea mays), 5'ss of exon 10
lower sequence: GLYMA06G09360.1 (Glycine max), 5'ss of exon 20

TCCTGCCGGTATAAATGCTCTATTTAGTCATGGACCGAGGCGGTCAGCAGGCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGTTgtaagtattcttcacccacctacatttcaatgttatatcggtatctatcacacgtaatggctg-caattgaatgagttttttttttctatcaagtgt--tgcgcctctataaccttcctgtgcttgttaatatctag----------------------
||||| ||| || |||||||| |||||||||||||| ||||| || ||||||||||| ||| | |||||  |||||||  | || ||  |  | |||||||| ||  || |  |   |  | ||  | | ||  ||||  | |||  |||       |   || ||     |||    |||   ||       |||   |       |||    |   | |   || |  || || |                      
TCCTGTCGGAATTAATGCTCTCTTTAGTCATGGACCAAGGCGTTCTGCAGGCCTTGCACGTCTTTATGCTCTGTGGAATCTTACATCCTTCATGAATGTTgtgag--ttatctattgaatttcagctgattgccatatttg-atccttcaatttatttatttgtcaggctgatggacctttccatttatcagggtggaatatagtaatatggattatatttttctggttttaactggggatcttgttatttgtcactag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|32930111|gb|CF034923.1|CF034923

EST:     GCCTTGCTCGTGTGTATGCATTGTGGAACATAACTCCACTGGCCAATGTT                         GTTGTGGCTTTCATATGTGGTTTTGT
genomic: GCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGTTgtaagtattc ... taatatctagGTTGTGGCTTTCATATGTGGTTTTGT

EST: gi|32916793|gb|CF021605.1|CF021605

EST:     GCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGAT                         GTTGTGGCTTTCATATGTGGTTTTGTACATTATAAGTTATCAACCAAAACA
genomic: GCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGTTgtaagtattc ... taatatctagGTTGTGGCTTTCATATGTGGTTTTGTACATTATAAGTTATCAACCAAAACA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 61

TCCTGCCGGTATAAATGCTCTATTTAGTCATGGACCGAGGCGGTCAGCAGGCCTTGCTCGTGTGTATGCATTGTGGAACATAACTTCACTGGTCAATGTTgtaagtattc ... taatatctagGTTGTGGCTTTCATATGTGGTTTTGTACATTATAAGTTATCAACCAAAACACACCCGAGCGTGCAGCCCTGGAACCTGGGAAC

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 85

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 78