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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctgttgctcaggtttatatggtcctgtttccccttgtttctgtcacctctgtgatattaatactatggttatttgggggggggggggtggcttattgcag

Basic information

species Zea mays
transcript GRMZM2G150251_T02
intron # 10
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G150251_T02 (Zea mays), 5'ss of exon 10
lower sequence: GLYMA14G21190.1 (Glycine max), 5'ss of exon 5
AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagt---tctgttgctcaggtttatatggtcctgtttccccttgtttctgtcacctct--gtgatattaatactatggttatttgggggggggggggtggcttattgcag-------------------------------------------------------------------------------
 ||||| |   ||   |  || ||||| || |||||||  |||||| | || ||  |||   | || |||||||| ||||||||||| |||||||| ||||||| |   ||||| | | |  ||||| |  |  | |||| | |  | | | | |       | ||| ||||   ||   | |  |             | |||  |||                                                                                  
TAGAGGAAAACAGCCCCAGTATTTCAAGAGTGCATTTTTAGGATACACTGTTGGCTTGGTCCTTACAATCATTGTGATGAACTGGTTCCAAGCTGCTCAGgtgattggatctgtagttaatttttat-taattttttttctcttgatattttttagtggaaggggattttaactgtaatatcaaattcactactaatattagctggttgagcatgtgaacatgccatggttttttgaggattgatcttcgctattaagtattaacttcttttccttttatctttgtttcag

upper sequence: GRMZM2G150251_T02 (Zea mays), 5'ss of exon 10
lower sequence: Vv06s0009g03520.t01 (Vitis vinifera), 5'ss of exon 10
AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagt---------tctgttgctcaggtttatatggt-----cctgt----------ttccccttgtttctgtcacctctgtgatattaatactatggttatttgggg--gggggggggtggcttattgcag---------------------------------------------------------------
 ||||| |    ||| |  ||||| || || ||||||| ||||||  |    ||| |||  || || ||  |||| |||||||||||||||||||||||||||| |         ||  || |||     ||||||       | |||          ||||| |||||    |    || ||          || |   | |||  |    | ||  | | |  |||| |                                                                 
CAGAGGGAAAGGGAACCAGTACTTTAAGAGTGCATTTTTGGGATATACAACCGGTTTGGTTGTAACAATTGTTGTCATGAACTGGTTTCAAGCTGCACAGgtgactactacaaaatccatttctctatcctatatgactgatacgtgtggagatatagttccctttgttgaatttcattcagtatctagctggctttaactgtttaagaccagtggaagttagagtatttccatgtgtgagttttctcgtgttattcactacttggtcctaactgttattctaacttcactttcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33092200|gb|CF052194.1|CF052194
EST:     TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAG                         CCGGCTCTACTGTACCTCG
genomic: TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCG
EST: gi|33093608|gb|CF053602.1|CF053602
EST:     TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAG                         CCGGCTCCACTGT
genomic: TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGT
EST: gi|32837706|gb|CD977384.1|CD977384
EST:     TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAG                         CCGGCTCTACTGTA
genomic: TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTA
EST: gi|30031839|gb|CB833690.1|CB833690
EST:     TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAG                         CCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC
genomic: TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC
EST: gi|78123104|gb|DV541488.1|DV541488
EST:     TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAG                         CCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC
genomic: TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC
EST: gi|60337558|gb|DN204531.1|DN204531
EST:     TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAG                         CCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC
genomic: TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC
EST: gi|76282340|gb|DV021908.1|DV021908
EST:     TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAG                         CCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC
genomic: TGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 228

AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACACTGTCTCTGGTACGGAGAAGTGAAACAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 108

AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACACTGTCTCTGGTACGGAGAAGTGAAACAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 85

AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACACTGTCTCTGGTACGGAGAAGTGAAACAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 205

AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctg ... cttattgcagCCGGCTCTACTGTATCTCGTTCCAGGTGTGATCGGATTCGTCGCCGTACACTGTCTCTGGTACGGAGAAGTGAAACAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AAGAGGTATCAAGAAGCGTTACTTCAACAGCGCATTTTCGGGATACGCCGTGGGTATGGCGGTGACCATCATTGTAATGAACTGGTTTCAAGCTGCACAGgtgagttctgttgctcaggtttatatggtcctgtttccccttgtttctgtcacctctgtgatattaatactatggttatttgggggggggggggtggcttattgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC