ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TTTCATATTGGCCTGCTGCATTATATACAGCTGTCCCCAGCCGCTCTTTCTTCCCAAGGGGATTTCTGTGGGATGAGGGCTTCCATCAGTTAGTCATTTGgtaactattactttttatctcagcgttgaatgaaggttttcttctgtctgctctaactcacatccttggtggtgatgtgcag

Basic information

species	Zea mays
transcript	GRMZM2G126468_T01
intron #	10
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G126468_T01 (Zea mays), 5'ss of exon 10
lower sequence: LOC_Os01g69210.1 (Oryza sativa), 5'ss of exon 10

TTTCATATTGGCCTGCTGCATTATATACAGCTGTCCCCAGCCGCTCTTTCTTCCCAAGGGGATTTCTGTGGGATGAGGGCTTCCATCAGTTAGTCATTTGgtaactattactttttatctcagcgttgaatgaaggttttcttctg-tctgctctaactcacatccttggtggtgatgtgcag---------------------------------------------
|  |||||||||| |||||| ||||||||||||| || || ||||| |||||||||||||||||||| ||||||||||||||||||||||||||||||||||||  ||| | |   ||||    ||  |  | |||  |||| |   | |    | |||| | |  ||  ||    ||||| |                                             
TCCCATATTGGCCAGCTGCACTATATACAGCTGTGCCTAGTCGCTCATTCTTCCCAAGGGGATTTCTATGGGATGAGGGCTTCCATCAGTTAGTCATTTGgtaagcatttcatcg-atcttgatgtgtagcgcagggcttctgccactatatcatcactcgctttttttttgaatctgtgctggtattattgctgcttgtttaacacatccttgatggtgatgtatag

upper sequence: GRMZM2G126468_T01 (Zea mays), 5'ss of exon 10
lower sequence: Vv01s0010g03300.t01 (Vitis vinifera), 5'ss of exon 10

TTTCATATTGGCCTGCTGCATTATATACAGCTGTCCCCAGCCGCTCTTTCTTCCCAAGGGGATTTCTGTGGGATGAGGGCTTCCATCAGTTAGTCATTTGgt-----aactattactttttatctcagcgttgaatgaaggttttcttctgt-ctgctc-----taactcacatccttggtggt---gatgtgcag----------------------------------------------------------------
||||||||||||| |||| | | || |||||||| || || || || ||||| |||||||||||| | |||||||| || || |||||| || | |||||||      | ||||| | |||   |  || ||   |      |  ||   || ||| |      | ||  ||||| || | |     | ||| ||                                                                 
TTTCATATTGGCCGGCTGAACTTTACACAGCTGTTCCAAGTCGATCCTTCTTTCCAAGGGGATTTTTATGGGATGAAGGTTTTCATCAGCTACTAATTTGgttagttgattattattgtttgcatttgcattttgttttttctccctcaagtgctggttatcaattacctacatctttagggaaataggtgttcattttgccacacattggaaataaatcttcctttgttccacttaacatatttctgactcttgaacag

upper sequence: GRMZM2G126468_T01 (Zea mays), 5'ss of exon 10
lower sequence: PP1S11_175V6.1 (Physcomitrella patens), 5'ss of exon 10

TTTCATATTGGCCTGCTGCATTATATACAGCTGTCCCCAGCCGCTCTTTCTTCCCAAGGGGATTTCTGTGGGATGAGGGCTTCCATCAGTTAGTCATTTGgtaac-----tattactttttatctcagcgttgaatgaaggttttct--tctgtctgctctaactcacatccttggtggtgatgtgcag---------------------------------------------------------------------------------------------------
    ||| ||||| || ||||| ||||| |||||||| |||||||||||||| ||| | || || || ||||| ||||| || |||||| |  |  |  |||||       ||   | | || ||||   ||| || |  |||| |   || |     |  ||       |   ||   |  | |   |                                                                                                   
GGAAATACTGGCCAGCGGCATTGTATACTGCTGTCCCTAGCCGCTCTTTCTTTCCACGTGGTTTCCTTTGGGACGAGGGTTTTCATCAGCTCTTGGTCAGgtaagaccggcatgtatgtatacctcatt-ttgtattagagtttgcgaatcagagaaatgcaagcagtgcctcggggtttcctttattgtgtctttaaggaaagttaaggaggtttctgtgttttgcaaaatccacagttgtggaaattttagtagaaagtacagcttataactcctaaatttcgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|89756733|gb|DY686652.1|DY686652

EST:     TTCCCAAGGGGATTTCTGTGGGATGAGGGCTTCCATCAGTTAGTCATTTG                         GCGTTGGGATGTGCATATATCTATGGATATCATTGGACATTGGTTAGATCT
genomic: TTCCCAAGGGGATTTCTGTGGGATGAGGGCTTCCATCAGTTAGTCATTTGgtaactatta ... tgatgtgcagGCGTTGGGATGTGCATATATCTATGGATATCATTGGACATTGGTTAGATCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 196

TTTCATATTGGCCTGCTGCATTATATACAGCTGTCCCCAGCCGCTCTTTCTTCCCAAGGGGATTTCTGTGGGATGAGGGCTTCCATCAGTTAGTCATTTGgtaactatta ... tgatgtgcagGCGTTGGGATGTGCATATATCTATGGATATCATTGGACATTGGTTAGATCTGCTTAATTCAGATGGATGGATTCCTAGGGAGCAAATCTTAGGAGCTGAA

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 154

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 174