ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

CCTCTTTTAATTGGCTCTGATGATAACGAAGAGGCAAATGCTAGAAGGGAAGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCTgtatgtggacgtacattagtctacattacattcttcttgttggtttccttgattatttatctgttattttttggttgttcacccttggaatag

Basic information

species	Zea mays
transcript	GRMZM2G048067_T03
intron #	10
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G048067_T03 (Zea mays), 5'ss of exon 10
lower sequence: LOC_Os12g01922.1 (Oryza sativa), 5'ss of exon 16

CCTCTTTTAATTGGCTCTGATGATAACGAAGAGGCAAATGCTAGAAGGGAAGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCTgtatgtggacgt---acattagtctacattacattcttcttgttggtttccttgattatttatctg--ttattttttggttgttcacccttggaatag---------------
|| ||| ||| ||| |||||||||||||| || |||||||||||||| |||||| |||||||| ||||| | ||||  || ||||| ||||| || || ||||||||| |     ||| || | ||||||||||| ||  | ||||  | ||   |||||||||||  | ||  | || ||   |  | |  |  |                 
CCACTTCTAACTGGTTCTGATGATAACGAGGAAGCAAATGCTAGAAGAGAAGAGCGAGAACGAATTGCAATGGATTGCATTGCTAAATGCCAACGATCATgtatgtggccaagcaacactattttacattacatttttgctattggagttct-atttatttatctgattaatgatatgcttccccttcttctgtgtgttggtggcattagcag

upper sequence: GRMZM2G048067_T03 (Zea mays), 5'ss of exon 10
lower sequence: LOC_Os11g01872.1 (Oryza sativa), 5'ss of exon 15

CCTCTTTTAATTGGCTCTGATGATAACGAAGAGGCAAATGCTAGAAGGGAAGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCTgtatgtggacgt---acattagtctacattacattcttcttgttggtttccttgattatttatctg--ttattttttggttgttcacccttggaatag---------------
|| ||| ||| ||| |||||||||||||| || |||||||||||||| |||||| |||||||| ||||| | ||||  || ||||| ||||| || || ||||||||| |     ||| || | ||||||| ||| ||  | ||||  | ||   |||||||||||  | ||  | || ||   |  | |  |  |                 
CCACTTCTAACTGGTTCTGATGATAACGAGGAAGCAAATGCTAGAAGAGAAGAGCGAGAACGAATTGCAATGGATTGCATTGCTAAATGCCAACGATCTTgtatgtggcctagcaacactattttacattatatttttgctattggagttct-atttatttatctgattaatgatatgcttccccttcttctgtgtgttggtggcattagcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|30087149|gb|CB885357.1|CB885357

EST:     AGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCT                         C---TTGCAAGATGACAAACCAAATTGCTAGTTGGGATACGAGGTTTGAGT
genomic: AGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCTgtatgtggac ... cttggaatagCAGCTTGCAAGATGACAAACCAAATTGCTAGTTGGGATACGAGGTTTGAGT

EST: gi|28576038|gb|CB281930.1|CB281930

EST:     AGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCT                         C---TTGCAAGATGACAAACCAAATTGCTAGTTGGGATACGAGGTTTGAGT
genomic: AGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCTgtatgtggac ... cttggaatagCAGCTTGCAAGATGACAAACCAAATTGCTAGTTGGGATACGAGGTTTGAGT

EST: gi|31558447|gb|CD527659.1|CD527659

EST:     AGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCT                         C---TTGCAAGATGACAAACCAAATTGCTAGTTGGGATACGAGGTTTGAGT
genomic: AGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCTgtatgtggac ... cttggaatagCAGCTTGCAAGATGACAAACCAAATTGCTAGTTGGGATACGAGGTTTGAGT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 130

CCTCTTTTAATTGGCTCTGATGATAACGAAGAGGCAAATGCTAGAAGGGAAGAGAGAGAACGACTTGCACTAGATTATATCGCTAAGTGCCAGCGCTCCTgtatgtggac ... cttggaatagCAGCTTGCAAGATGACAAACCAAATTGCTAGTTGGGATACGAGGTTTGAGTTGGGAACAAAAACAGCTTTATTGTTGCCATTTTCTCCGATCGTTGTTGC

Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 86

Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 76