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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcagagttctcagtcaatataatatttatcgtattttggttcaccaactaatgccaggccaagtttcttaccagtggaatgcatttttttag

Basic information

species Zea mays
transcript GRMZM2G013908_T01
intron # 10
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G013908_T01 (Zea mays), 5'ss of exon 10
lower sequence: LOC_Os01g32750.1 (Oryza sativa), 5'ss of exon 10
TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttca-gagttctcagtcaatataatatttatcgtattttggttcaccaactaatgccaggccaagtttcttaccagtggaatgcatttttttag
 | |||||| ||||| ||| | ||||||||| | || ||||| ||||||||||| ||||||||||| |||||||| |||||||||||||||||| ||||||| || | |  || | ||| | |||| |    ||||| ||||||  | ||||| ||                ||||||| |||||||| ||||||    
CGAGACATACATGCAGCTTTTAGAGCCGGAATTACAACTTGATAAGCAGAAAAATGAGATGAAAAGAAAGGAAGCATGGCGTGTTTATGGTGCCCTGCTGgttagattacaagctttcaatgaatacatg--ttatc-tatttttatgcaccagct----------------tcttacctgtggaatgtatttttcag-

upper sequence: GRMZM2G013908_T01 (Zea mays), 5'ss of exon 10
lower sequence: GLYMA18G52720.1 (Glycine max), 5'ss of exon 5
TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcagagttctcagtcaatataatatttatcgtattttggttcaccaactaatgccaggccaagtttctta-ccagtggaatgcatttttttag--------------------------
||  |||||||||| |||||| || || ||  |||  ||||||| ||||||||| || ||||||||| | ||||||||||  |||||||| ||||||||||| || |  |   || ||   |  | | |  |||    |   | | | |      ||    ||      | ||| |   |||  |||    || ||  |                          
TGAAACATATATGCGACTTCTTGAACCAGAGATGCTTCTTGAGATGCAGAAAAATGAAATGAAAAGGCATGAAGCTTGGCTGGTTTATGGAGCCTTGCTGgtaagatatgctgtcacatatag-acacttgttagtaaaaaataggtgattttacaactgaagaatgtctgtctcagttagtataatatggttgttctgataattgtatgtaacgtatcattcag

upper sequence: GRMZM2G013908_T01 (Zea mays), 5'ss of exon 10
lower sequence: Vv12s0059g00300.t01 (Vitis vinifera), 5'ss of exon 10
TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcagagttctca-gtcaatataatatttatcgtattttggttcaccaactaatgccaggccaagtttcttaccagtggaatgcatttttttag--------------
||   ||||  | | |||||| || || ||  |||  |||||||||||||| || ||| | || ||| | ||||| ||| |||||||||| ||||||||||| ||||    ||||| | ||   | |||| ||   |    |  |     | ||     || |    | ||| | |   |   |||    ||| |||                
TGAACCATACCTTCGACTTCTTGAACCTGAGATGCTCCTTGAGAAGCAGAAGAATGAGCTAAAGAGGCATGAAGCATGGTGTGTTTATGGGGCCTTGCTGgtgagtttt--gttctaatgtgtttgtaatgttatccagtatgagaagtcctaagatgggcta---tatgttgcatgtaatgagaacatgtttcttttattctttctttctag

upper sequence: GRMZM2G013908_T01 (Zea mays), 5'ss of exon 10
lower sequence: Vv19s0090g01310.t01 (Vitis vinifera), 5'ss of exon 10
TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcagagttctcagtcaatataatattta--tcgtattttggttcaccaactaatgccaggccaagtttcttaccagtggaatgcatttttttag
|| | ||||  ||| |||||| ||||| ||| |||||||||||||||| || || | |||||| ||  | ||||| |||||||| ||||| ||||| ||||| ||||     |||| | ||  ||     ||||  | | |     |      |  |  || |   |   |   || ||  ||  |    |  |      
TGAGCCATACTTGCGACTTCTTGAGCCAGAAATGCAGCTTGAGAAGCAAAAGAATGGGATGAAGAGACATGAAGCCTGGCGTGTATATGGAGCCTTACTGgtgagtttg--tttctaaatctctagttggtttaatttgaaaggaagccactgagatggtggccatcagtggagctaacatgtcca-catacctcccag-

upper sequence: GRMZM2G013908_T01 (Zea mays), 5'ss of exon 10
lower sequence: Vv12s0059g00410.t01 (Vitis vinifera), 5'ss of exon 3
TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcagagttctcagtcaatataatatttatcgtattttggttcaccaactaatgccaggc-caagtttcttaccagtggaatgcatttttttag--------------
||   ||||  | | |||||| || || ||  |||  |||||||||||||| || ||||| || ||| | ||||| |||||||||||||| |||||||||||  |||    |||   | |   | |   || |     | | ||      |  ||||     |    | ||| | |   |   |||    ||| |||                
TGAACCATACCTTCGACTTCTTGAACCTGAGATGCTCCTTGAGAAGCAGAAGAATGAGATAAAGAGGCATGAAGCATGGCGTGTTTATGGGGCCTTGCTGgtgcgtttt--gtttctaatgggtttgtaatgttatccagtatgagaagtc--ctaagatgggttatatgttgcatgtaacgagaacatgtttcttttattctttctttctag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|26457231|gb|CA828814.1|CA828814
EST:     AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTG                         TGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
genomic: AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
EST: gi|26455852|gb|CA827435.1|CA827435
EST:     AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTG                         TGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
genomic: AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
EST: gi|19822284|gb|BQ048308.1|BQ048308
EST:     AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTG                         TGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
genomic: AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
EST: gi|76283812|gb|DV023380.1|DV023380
EST:     AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTG                         TGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
genomic: AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
EST: gi|211271502|gb|FL060904.1|FL060904
EST:     AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTG                         TGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG
genomic: AAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 182

TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTGCTTTCTCCGTCGATGCGGCCTCTTTTACAGAGCAACAAAAGGGTCTTAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 101

TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTGCTTTCTCCGTCGATGCGGCCTCTTTTACAGAGCAACAAAAGGGTCTTAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 100

TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTGCTTTCTCCGTCGATGCGGCCTCTTTTACAGAGCAACAAAAGGGTCTTAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 99

TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcag ... atttttttagTGTGCTGCAGGCAAATGCTTGTATGATCGGTTGAAACTATTTCCTGGTTTGCTTTCTCCGTCGATGCGGCCTCTTTTACAGAGCAACAAAAGGGTCTTAA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TGTGACATATATGCAACTTCTGGAGCCGGAACTGCAGCTTGAGAAGCAGAAAAACGAGATGAAAAGGAAGGAAGCTTGGCGTGTTTATGGTGCCTTGCTGgtcagttcagagttctcagtcaatataatatttatcgtattttggttcaccaactaatgccaggccaagtttcttaccagtggaatgcatttttttag

- - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA