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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttccttgcaccaaaaccaaacttaccccctgttttcatggattgatggttgtcctagtgatcagattttgttgcggtggcccgattctatcgttc...

Basic information

species Zea mays
transcript GRMZM5G882228_T02
intron # 1
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G882228_T02 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os10g35870.1 (Oryza sativa), 5'ss of exon 1
AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttccttgcaccaaaaccaaacttaccccctgttttcatggattgatggttgtcctagtgat--cagattttgttgcggtggcccgattctatcgttc--
|||||||||||||||| ||||||||||||||||| ||||| || |||||||| ||||||||||||||||||||||||||||| |||||||| ||||||||||| ||||||| |  |    || || | |   ||| ||||| |   | ||| | |||  |  || |  |   ||  |  | ||| ||  | |||||  ||    
AGGTCTCCGAGGAGGAGCTCCGCCAGTACGACGGGTCCGATCCCAAGAAGCCCCTCCTCATGGCCATCAAGGGCCAGATCTATGACGTCACGCAGAGCAGgtccgttccttacgtct---ccgaaatcatatccttttttcctttgt-gatcgctgtgatgatgctttcgatttgggaggtggttgcatgtttctactgtagtc

upper sequence: GRMZM5G882228_T02 (Zea mays), 5'ss of exon 1
lower sequence: GLYMA09G25940.1 (Glycine max), 5'ss of exon 1
AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttccttgcaccaaaaccaaacttaccccctgttttcatggattgatggttgtcctagtgatcagattttgttgcggtggcccgattctatcgttc---
||||| ||||||| ||||||      ||||||||| |||| ||  ||||||| ||||||||||||||||||||||||||||| || ||  |||||||||||| |  |  || || || | |   | ||   ||   |   |||      |      |  |  || |   ||||| ||    |       || | |  ||    
AGGTCACCGAGGACGACCTCAAGGCCTACGACGGCACCGATCCCGAGAAGCCTCTCCTCATGGCCATCAAGGGCCAGATCTATGATGTTTCCCAGAGCAGgtatagttatt-catcatagctttaattgaaccaactcagcataacaaaacacccctttt--tgtttttatttttttattttttttgagttttgttctttttg

upper sequence: GRMZM5G882228_T02 (Zea mays), 5'ss of exon 1
lower sequence: GLYMA20G29660.1 (Glycine max), 5'ss of exon 1
AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttccttgcaccaaaaccaaac--ttacccc---ctgttttcatggattgatggttgtcctagtgatcagattttgttgcggtggcccgattctatcgttc
|| || | | |||||| |||      ||||||||| |||| ||  |||||||  | |||||||||||||||| ||||||||| || ||| |||||||||||| |  |   | ||| ||| |||  |  ||   ||    ||  ||  |  | |  || |||||     | |||  ||  |||  ||       |||   |  |  
AGATCACAGCGGAGGAGCTCAAGGCCTACGACGGCACCGATCCCGAGAAGCCCTTGCTCATGGCCATCAAGGCCCAGATCTATGATGTCTCCCAGAGCAGgtttactgtct-cacaaaacccacccctttgttccaaattgcatttttttaattttgattgtct--acgctcattttaggttttgggtattttatttatttat--

upper sequence: GRMZM5G882228_T02 (Zea mays), 5'ss of exon 1
lower sequence: GLYMA10G38150.1 (Glycine max), 5'ss of exon 1
AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgtt-ccttgcaccaaaaccaaacttaccccctgttttcatggattgatggttgtcctagtgatcagattttgttgcggtggcccgattctatcgttc---
|| || | | |||||| |||      ||||||||| |||| ||  |||||||  | |||||||||||||||| ||||||||| || ||| |||||||||||| |  |  ||  ||  ||| |||| | |     ||   ||     |||  ||  |||  |  |      |||||| ||   |   |  ||| ||   |     
AGATCACGGCGGAGGAGCTCAAGGCCTACGACGGCACCGATCCCGAGAAGCCCTTGCTCATGGCCATCAAGGCCCAGATCTATGATGTCTCCCAGAGCAGgtttactgtctcacataaaacccaaccctttgttctaaattg--caatttttg--tgtttttttctcttcattttgatggtctatgctcattttaggttgtggg

upper sequence: GRMZM5G882228_T02 (Zea mays), 5'ss of exon 1
lower sequence: EFJ25427 (Selaginella moellendorffii), 5'ss of exon 1
AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttccttgcaccaaaaccaaacttaccccctgttttcatggattgatggttgtcctagtgatcagattttgttgcggtggcccgattctatcgttc
||||| | || ||||| ||| ||   ||||| |||   ||       |||||||| |||||||||||||||||||| ||||| |||||| ||  |  |||||               | |  | | |   ||||  ||    ||      |||| ||  |  |    ||||||                           
AGGTCACGGAAGAGGAGCTCAGCGCCTACGATGGCAAGGATGACTCCAAGCCGCTGCTCATGGCCATCAAGGGCCAAATCTATGACGTCTCCATGTCCAGgt---------------agaagagattcttccccatttcgtttgtgccactggt-gtgatctttgggagatttcag------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|31911267|gb|CD651839.1|CD651839
EST:     CGCTCCTCATGGCCATCAAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CGCTCCTCATGGCCATC-AAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|60340782|gb|DN207755.1|DN207755
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|4646507|gb|AI491646.2|AI491646
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATTTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|211122173|gb|FL463029.1|FL463029
EST:     CCGCTCCTCATGGCCATCAAGGSCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACYTGGCGGACYTTACGCCCTGTTCGCCGGCAAAGATSC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|32805639|gb|CD957873.1|CD957873
EST:     GATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCCGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: GATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|60347796|gb|DN214769.1|DN214769
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|101391073|gb|EB817470.1|EB817470
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|211479773|gb|FL191755.1|FL191755
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCT
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCT
EST: gi|211020500|gb|FL191505.1|FL191505
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAR                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|148987509|gb|EE031367.2|EE031367
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|211353843|gb|FL191723.1|FL191723
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACG
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACG
EST: gi|211127597|gb|FL191533.1|FL191533
EST:     AAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: AAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|15079834|gb|BI388850.1|BI388850
EST:     GACGTCACCCAGAGCAG                         AATGTTCTATGGACCCGGCGGACCTTACGCCCTGTTCGCTGGCAAAGATGC
genomic: GACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|148990761|gb|EE032172.2|EE032172
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|211346636|gb|FL191687.1|FL191687
EST:     AGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: AGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|26557496|gb|CA829731.1|CA829731
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|60338776|gb|DN205749.1|DN205749
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|32805838|gb|CD958072.1|CD958072
EST:     GATCTCCGACGTCACCCAGAGCAG                         AATGTTCTATGGACCCGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: GATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|29543458|gb|CB603854.1|CB603854
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|148944920|gb|EC890079.2|EC890079
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|149106527|gb|EE188419.2|EE188419
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|32808118|gb|CD960352.1|CD960352
EST:     GATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCCGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: GATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|148954008|gb|EC899401.2|EC899401
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|148950410|gb|EC895977.2|EC895977
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATTTATGACGTCCCCCAGAGCAG                         AATGTTTTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|20208093|gb|BQ134182.1|BQ134182
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|32830557|gb|CD970235.1|CD970235
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCCGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|83278996|gb|DV943004.1|DV943004
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
EST: gi|148944919|gb|EC890078.2|EC890078
EST:     CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAG                         AATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC
genomic: CCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 274

AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGCCAGCAGAGCTCTAGCGAAGATGTCGTTCGAGCCACAGGACCTCACCGGC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 272

AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGCCAGCAGAGCTCTAGCGAAGATGTCGTTCGAGCCACAGGACCTCACCGGC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 226

AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGCCAGCAGAGCTCTAGCGAAGATGTCGTTCGAGCCACAGGACCTCACCGGC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 246

AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttcct ... ccctacgcagAATGTTCTATGGACCTGGCGGACCTTACGCCCTGTTCGCCGGCAAAGATGCCAGCAGAGCTCTAGCGAAGATGTCGTTCGAGCCACAGGACCTCACCGGC


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AGGTCTCCGAGGAGGACCTCCGCCAGTACGACGGCTCCGACCCTAAGAAGCCGCTCCTCATGGCCATCAAGGGCCAGATCTACGACGTCACCCAGAGCAGgtctgttccttgcaccaaaaccaaacttaccccctgttttcatggattgatggttgtcctagtgatcagattttgttgcggtggcccgattctatcgttc

- - - - - - - - - - - - - - - - - - - - - - - - - -  CTCCTC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aaaaccaa