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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GCGACGCCCACGACGGCCGTGTCATCTTCCGGCGTGGCCGCGCAGCTGCCGCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgatatttttttgttttgttttgtttttagtcgtttatagtggtccgaggtgtgtgtgcgtgccggggatagctagctctttgtcgtgatcgatggcgacggcgccgtggccgtggtggtggtggcgcag

Basic information

species Zea mays
transcript GRMZM2G171073_T02
intron # 1
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G171073_T02 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os03g10140.1 (Oryza sativa), 5'ss of exon 1
GCGACGCCCACGACGGCCGTGT-CATCTTCCGGCGTGGCCGCGCAGCTGCCGCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgatatttttttgttttgttttgtttttagtcgtttatagtggtccgaggtgtgtgtgcgtgccggggatagctagctctttgtcgtgatcgatggcgacggcgccgtggccgtggtggtggtggcgcag
||   || |  | ||| || |  |      | ||  | |  ||| || ||||| |||    ||||| |||||||| |||||||| |||||||||||||||||||| |||        |  ||     |       || | |||   ||    ||| | || |||          || || || |  | |  |    || || |  ||     | |  |||    ||||||| ||||
GCCGAGCGCGGGGCGGGCGCGGGCGCGCAGCAGCA-GCCTCCGCCGCAGCCGCAGCCGCAGCCGCCGGCCAAGAAGAAGCGTGCACTCCCCGGCATGCCAGgtgactgac-------tagttaaccgtacc---ggttgcttaatttgactagagttatgcgtg----------atggcgagatgatcgatggcgacggtgac--cgatcgagcgttcgttcgcgtggtggtgcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|91051718|gb|EB162136.1|EB162136
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|211276573|gb|FL135681.1|FL135681
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|211274764|gb|FL135676.1|FL135676
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|86466530|gb|DY232900.1|DY232900
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCGTGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|149079811|gb|EE168809.2|EE168809
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|149004453|gb|EE039076.2|EE039076
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|78106463|gb|DV524881.1|DV524881
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|211276575|gb|FL135683.1|FL135683
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|78109193|gb|DV527610.1|DV527610
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|91048119|gb|EB158537.1|EB158537
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|71439765|gb|DR820815.1|DR820815
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|148966765|gb|EE017851.2|EE017851
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|148973264|gb|EE024343.2|EE024343
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|148989685|gb|EE031768.2|EE031768
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|211274761|gb|FL135673.1|FL135673
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|76913956|gb|DV165339.1|DV165339
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|78112595|gb|DV530989.1|DV530989
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|211274767|gb|FL135679.1|FL135679
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|76014993|gb|DT942163.1|DT942163
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|76015015|gb|DT942185.1|DT942185
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|71313169|gb|DR793116.1|DR793116
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|149037291|gb|EE155335.2|EE155335
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|78103895|gb|DV522318.1|DV522318
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|211276576|gb|FL135684.1|FL135684
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|71437227|gb|DR818277.1|DR818277
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|71424567|gb|DR806363.1|DR806363
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|149098029|gb|EE183976.2|EE183976
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|74243979|gb|DT651893.1|DT651893
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|71323979|gb|DR798675.1|DR798675
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|71444559|gb|DR825609.1|DR825609
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|148945429|gb|EC890660.2|EC890660
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|211274763|gb|FL135675.1|FL135675
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
EST: gi|71437740|gb|DR818790.1|DR818790
EST:     GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAG                         ATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA
genomic: GCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 41 (downstream exon)
Score: 54

GCGACGCCCACGACGGCCGTGTCATCTTCCGGCGTGGCCGCGCAGCTGCCGCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGAACCGGTTCGTGTGCGAGATCTGCAACAAGGGGTTCCAGCGGGACCAGAA


Block sizes: 38 (upstream exon), 49 (downstream exon)
Score: 13

GCGACGCCCACGACGGCCGTGTCATCTTCCGGCGTGGCCGCGCAGCTGCCGCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGAACCGGTTCGTGTGCGAGATCTGCAACAAGGGGTTCCAGCGGGACCAGAA


Block sizes: 41 (upstream exon), 31 (downstream exon)
Score: 9

GCGACGCCCACGACGGCCGTGTCATCTTCCGGCGTGGCCGCGCAGCTGCCGCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGAACCGGTTCGTGTGCGAGATCTGCAACAAGGGGTTCCAGCGGGACCAGAA


Block sizes: 41 (upstream exon), 31 (downstream exon)
Score: 10

GCGACGCCCACGACGGCCGTGTCATCTTCCGGCGTGGCCGCGCAGCTGCCGCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgata ... ggtggcgcagATCCTGACGCGGAGGTGATCGCGCTGTCGCCCAAGACGCTGCTGGCGACGAACCGGTTCGTGTGCGAGATCTGCAACAAGGGGTTCCAGCGGGACCAGAA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GCGACGCCCACGACGGCCGTGTCATCTTCCGGCGTGGCCGCGCAGCTGCCGCCGCCCGGCCCGCCCGCCAAGAAAAAGCGTGCCCTCCCCGGCATGCCAGgtgattgatatttttttgttttgttttgtttttagtcgtttatagtggtccgaggtgtgtgtgcgtgccggggatagctagctctttgtcgtgatcgatggcgacggcgccgtggccgtggtggtggtggcgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA