ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TTCATTTCATACTCCATTGTTTGTAGGTGAGAGAGGAGACCTACACTGCCTTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagttccttttatcatgcttcaaccatagtgcatgcatgactcttgcaacggttgagattatcttgttctgttgataaaatggtagggtggatgg...

Basic information

species	Zea mays
transcript	GRMZM2G161418_T01
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G161418_T01 (Zea mays), 5'ss of exon 1
lower sequence: GLYMA03G04500.1 (Glycine max), 5'ss of exon 9

TTCATTTCATACTCCATTGTTTGTAGGTGAGAGAGGAGACCTACACTGCCTTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagttccttttatcatgcttcaaccatagtgcatgcatgactcttgca-acggttgagattatcttgttctgttgataaaatggtagggtggatgg-
                          |||||||| ||||| ||||||||||| |||||||| |||||||| ||  | ||||| || ||||  || |||||||||| | ||   |||| |||  || |    |   | |   |  ||      |      |   |  || |||||        | |||  ||   |  ||   
--------------------------GTGAGAGATGAGACTTACACTGCCTTTGAAAACATATATCCTGTGCTTACGGAGTTCAGGAAAAACCAACAATGgtatgaatttgtcttta-catatttaactttttcagtacatagtacatacagatgtaacttcaacgattttgtttacccatcagaattataaa-tacatcaa

upper sequence: GRMZM2G161418_T01 (Zea mays), 5'ss of exon 1
lower sequence: GLYMA16G25450.3 (Glycine max), 5'ss of exon 9

TTCATTTCATACTCCATTGTTTGTAGGTGAGAGAGGAGACCTACACTGCCTTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagttccttttatcatgcttcaaccat--agtgcatgcatgactc-ttgcaacggttgagattatcttgttctgttgataaaatggtagggtggatgg
                          |||||||| ||||| ||||||||||| |||||||| ||||| || ||  | ||||| || ||||  || |||||||||| ||||   |||  |||  || |    |  ||| ||| ||    |  || ||||| ||  |  ||  |  |  |  | ||||||   |    |  |   
--------------------------GTGAGAGATGAGACTTACACTGCCTTTGAAAACATATATCCCGTGCTTACTGAGTTCAGGAAAAACCAACAATGgtatgaagttgtctttg-catatttaactttttcagtacatacagatgtaatttcaacgattttgtatag-tcatcataatcataaaagcattacatttaga-

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211401652|gb|FL441809.1|FL441809

EST:     TTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATG                         ATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGG
genomic: TTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagtt ... ctctttgcagATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGG

EST: gi|91052132|gb|EB162550.1|EB162550

EST:     TTCGAAAACATATATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATG                         ATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGG
genomic: TTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagtt ... ctctttgcagATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGG

EST: gi|149110160|gb|EE293976.2|EE293976

EST:     AGAGTTTAGAAAAATTCAGCAATG                         ATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGG
genomic: AGAGTTTAGAAAAATTCAGCAATGgtatgtagtt ... ctctttgcagATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGG

EST: gi|113613005|gb|DW775593.1|DW775593

EST:     TTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATG                         ATGACATGTGATGTGAAGGTCACGTTCTTCAAATTA
genomic: TTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagtt ... ctctttgcagATGACATGTGATGTGAAGGTCACGTTCTTCAAATTA

EST: gi|8930534|gb|BE225298.1|BE225298

EST:     TTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATG                         ATGACATGTGATGTGAAGGTCACGTTTTTCAAATTACAAGCTTCCTCGAGG
genomic: TTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagtt ... ctctttgcagATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 45 (upstream exon), 48 (downstream exon)
Score: 73

TTCATTTCATACTCCATTGTTTGTAGGTGAGAGAGGAGACCTACACTGCCTTCGAAAACATCTATCCTGTACTGTCAGAGTTTAGAAAAATTCAGCAATGgtatgtagtt ... ctctttgcagATGACATGTGATGTGAAGGTCACGTTCTTCAAATTACAAGCTTCCTCGAGGATTCTCGTGTGACCATGAATTCTAAATGTACATAAAAGTCTGGCGGAAC

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 47

Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 92