ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TGCGGCTAGCTCAACCATGGCGGACTCCAAGGCCACCTCGGCGGTCACCCTCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgacgtccatcagaccaacaccttccaatcattccatcggtgctccccgtctcgtccccatctctcactaaatctctgcctctcctccctcattcttgcag

Basic information

species	Zea mays
transcript	GRMZM2G151252_T03
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G151252_T01 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os01g52490.1 (Oryza sativa), 5'ss of exon 1

TGCGGCTAGCTC-AACCATGGCGGACTCCAAGGCCACCTCGGCGGTCACCCTCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgac--gtccatcagaccaacaccttccaatcatt-ccatcggtgctccccgtctcgtccccatctctcactaaatctctgcctctcctccctcattcttgcag
 ||||| | ||| | ||||| |||||||||||||| |  ||||||||||||||||||| ||||||||||||||||||||||| ||  ||||||| ||||| || || | |  ||||||      | | | | |   || || ||  |   ||||   |  |  || |  |||||| ||  ||   | | |  | | |             
-GCGGCCAACTCCAGCCATGTCGGACTCCAAGGCCGCGGCGGCGGTCACCCTCCGCACCCGCAAGTTCATGACCAACCGCCTCCTCTCCCGCAAGCAATTCgtgagcgccctgtccattctcaaagctcttcctcctccttgccgccatggctctgtgattgatctctctctctcgctggatgaatccttaccttgcag-----------

upper sequence: GRMZM2G151252_T01 (Zea mays), 5'ss of exon 1
lower sequence: EDP07861 (Chlamydomonas reinhardtii), 5'ss of exon 1

TGCGGCTAGCTCAACCATGGCGGACTCCAAGGCCACCTCGGCGGTCACCCTCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgacgtccatcagaccaacaccttccaatcattccatcggtgctccccgtctcgtccccatctctcactaaatctctgcctctcctccctcattcttgcag
                  |||    |    | | |    | ||  |||  |||||||||||||||||||||||||||| ||||||   |||   ||||| || |     ||    ||     |   || |        ||    |  ||    | || | | |   ||||||  | || |  ||                      
-----------------CGGCAAGATGTCGGACAA----GACGTGCACTATCCGCACTCGCAAGTTCATGACCAACCGGCTGCTGCAGCGCCGGCAATTCgtga-----gtttggcatggagaaggctgctggctgg-ccgcgcgctctggaggcctggacgctgactctcatcatat-tgcgcag--------------------

upper sequence: GRMZM2G151252_T01 (Zea mays), 5'ss of exon 1
lower sequence: PP1S450_3V6.1 (Physcomitrella patens), 5'ss of exon 1

TGCGGCTAGCTCAACCATGGCGGACTCCAAGGCCACCTCGGCGGTCACCCTCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgac---------gtccatcagaccaacacct--tccaatcattccatcggtgctccccgtctcgtccccatctctcactaaatctctgcctctcctccctcattcttgcag--------------------
                            | |||   |   ||   |||| |||||||| | |||||||||||||||||||||||||| || |||||||| ||||| |           || | || |        |   ||  |   ||  ||  |     || || | ||     | |   | |    |           |  |||| | ||  |                     
----------------------------ATGGCGGACAAAGCTTGCACCGTCCGCACTAGAAAGTTCATGACCAACCGCCTGCTGGCGCGGAAACAATTCgtaagcgcttttgagcttgttcttctggtttttgtcccgtctcaagtttttatgtgattgtccggttttgtttgggtttaggatttgggcgtgtttgggtatttctcaatttttgacctctttcggtgctttggcag

upper sequence: GRMZM2G151252_T01 (Zea mays), 5'ss of exon 1
lower sequence: PP1S13_236V6.1 (Physcomitrella patens), 5'ss of exon 1

-TGCGGCTAGCTCAACCATGGCGGACTCCAAGGCCACCTCGGCGGTCACCCTCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgacgtccatcagaccaacaccttccaatcattccatcggtgctccccgtctcgtccccatctctcactaaatctctgcc----tctcctccctcattcttgcag------------
  ||        ||||| |  | | | ||| |||       |||  |||| | || ||| | |||||||||||||| ||||| ||||| ||||| ||||| |||||||    |  ||  |    |  ||    |||  | || |  || ||   || | |     |     | ||  |  | | ||    |||  |   | | | |||               
AGGCCTTCGCGGCAACC-TCACCGTCACCATGGCGGAGAAAGCGTGCACCGTTCGTACTAGAAAGTTCATGACCAATCGCCTTCTGGCTCGCAAGCAATTCgtaagtgctccctctctcattttcgtctggttatc--ttcagctatgttcagggtttggggtttaggaaacgcttgaattttacccgattctgatgaatgagttttgtttctgtttgggcag

upper sequence: GRMZM2G151252_T01 (Zea mays), 5'ss of exon 1
lower sequence: PP1S450_9V6.1 (Physcomitrella patens), 5'ss of exon 1

---TGCGGCTAGCTCAACCATGGCGGACTCCAAGGCCACCTCGGCGGTCACCCTCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgacg--tccatcagaccaacaccttc----caatcat-tccatcggtgctccccg--tctcgtccccatctctcactaaatctctgc------ctctcctccctcattcttgcag
       ||| ||||   ||  ||     ||| |||   |   ||   |||| ||||||| || |||||||||||||||||||| ||||| ||||| ||||| ||||| ||||  | | |||         | ||    |||  || || |   | | | |  |  | | |      ||||  | | | | |  |        |||  |      |  | ||||
CGGCATCGCTCGCTCGCTCACTGCAA---CCATGGCGGACAAAGCTTGCACCGTCCGCACCCGGAAGTTCATGACCAACCGCCTTCTGGCGCGCAAGCAATTCgtaagcgacgagttcgtcatttttttctcctctctgcaaggatgtctacgtgagttgcttgagtgttggatttttctcgtattgattatgggaagggcgttctgtttgtgtgtgttggcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|32863164|gb|CF002846.1|CF002846

EST:     TCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTT                         GTGCTTGAGGTTATCCACCCCGGCCG
genomic: TCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgacg ... attcttgcagGTGCTTGAGGTTATCCACCCCGGCCG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1042

TGCGGCTAGCTCAACCATGGCGGACTCCAAGGCCACCTCGGCGGTCACCCTCCGCACTCGCAAGTTCATGACCAACCGCCTGCTGGCCCGCAAACAATTTgtaagtgacg ... attcttgcagGTGCTTGAGGTTATCCACCCCGGCCGCGCCAACGTCTCCAAGGTGAGATATCGGCCCCGGTACTGTGTGCGCGGCTCATATGGCGGGAGCTTCTGGCCTG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 650

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 679