ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

GTGGCGGCCGCCATCTTCGTCGTCCTCATGCTCGTCACGCCCGCCATCCCGCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgccctctaccctccaccatccttccccacccacgcgcgcagcacgcctcgattcgaccgccttcggcgcctcaatcgctgctccccatatcct...

Basic information

species	Zea mays
transcript	GRMZM2G147709_T01
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G147709_T01 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os01g73120.1 (Oryza sativa), 5'ss of exon 1

GTGGCGGCCGCCATCTTCGTCGTCCTCATGCTCGTCACGCCCGCCATCCCGCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgccctctaccctccaccatccttccccacccacgcgcgcagcacgcctcgattcgaccgcc-ttcggcg-cctcaatcgctgctccccatatcct
| ||||| | ||||||||||||| |||||||||||||| || |||||||||||| |||| |  |||||||||||||| |||||||||  ||| ||| | ||||   | |   | | ||  | |   ||  | | | ||  | ||| | |   ||| |   |  ||     ||| || || |  |        ||| | ||  
GCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGgtatgccacaaaccaaccctccctccccgccgcaaacctagggcggaccttcccttgcggccgccatagctcgccgtccgcggtattgagctcccgtctc--

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|60352475|gb|DN219448.1|DN219448

EST:     GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|211225144|gb|FL356495.1|FL356495

EST:     GCA-GACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAGACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|211166572|gb|FK969120.1|FK969120

EST:     GCA-GACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAGACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|211218284|gb|FL207814.1|FL207814

EST:     GCA-GACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|211515170|gb|FL354450.1|FL354450

EST:     GCAG-ACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAGACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|211166571|gb|FK969119.1|FK969119

EST:     GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|6695713|gb|AW288791.1|AW288791

EST:     CGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: CGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|78180569|gb|DV550897.1|DV550897

EST:     GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|211049503|gb|FL332054.1|FL332054

EST:     GCA-GACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAGACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|12046333|gb|BF728472.1|BF728472

EST:     GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|12045665|gb|BF727804.1|BF727804

EST:     GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

EST: gi|78180036|gb|DV550409.1|DV550409

EST:     GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAG                         GGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG
genomic: GCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 49

GTGGCGGCCGCCATCTTCGTCGTCCTCATGCTCGTCACGCCCGCCATCCCGCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgcc ... attactgcagGGATTCCCAACACCTTGAATGTGATCTCAAACATCCCTTTCTTCTTTGTTGGCGTCACGGGGCTCATCCTGTGCCACTTCAACAACTATTTCAGGCTAAG

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 85

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 82

Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 60

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTGGCGGCCGCCATCTTCGTCGTCCTCATGCTCGTCACGCCCGCCATCCCGCAGGACGAAGCCTACCACGACTTCGCGGACCAGCGCACCCTTTTCTTAGgtagcgcgccctctaccctccaccatccttccccacccacgcgcgcagcacgcctcgattcgaccgccttcggcgcctcaatcgctgctccccatatcct

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - cgccctc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - cctccacc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ccccacc