ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

GCACCTCGATGCCCCCTTATTCGGCGCCGATCTCATCCCCTCCGGCCGCCTCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcataccattgcattgcattgcaaagagacagacggttgctcttcgccatctcgcttgcttacacacatcaaccgcatcgatccatggcctgcgg...

Basic information

species	Zea mays
transcript	GRMZM2G122223_T01
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G122223_T01 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os01g49770.1 (Oryza sativa), 5'ss of exon 2

GCACCTCGATGCCCCCTTATTCGGCGCCGATCTCATCCCCTCCGGCCGCCTCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcataccattgcattgcattgcaaagagacagacggttgctc-ttcgccatctcgcttgcttacacacatcaaccgcatcgatccatggcctgcgg
 | |  ||  || ||||| ||||||||||| ||| |||||||||||||||||||||||||||||||||  |||||||||||||  || |||||||||||||||    |   ||  |  |  |  |   |  ||    ||   | ||    |   | | | |      ||| || |      | |     | | |||  |  
CCTCGACGTCGCGCCCTTCTTCGGCGCCGACCTCCTCCCCTCCGGCCGCCTCCGCCGGATGCGCGGGTACCGCCACTCCCCCGTCGGCCTCGAGGAAGAGgtaaaaaacgtcacaggctcacggtagtagcagcagtactagtactggcacagtagcgcacactgcaacaaacgttcgtt-cgtttcctcgttgcccccac

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211286951|gb|FL445024.1|FL445024

EST:     GACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: GACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|91048468|gb|EB158886.1|EB158886

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|211136816|gb|FL064025.1|FL064025

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|211136815|gb|FL064024.1|FL064024

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|211136814|gb|FL064023.1|FL064023

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|9733598|gb|BE512350.1|BE512350

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|76933767|gb|DV173581.1|DV173581

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|211136817|gb|FL064026.1|FL064026

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATATGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|9731032|gb|BE509784.1|BE509784

EST:     CCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: CCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|40334279|gb|CK368349.1|CK368349

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|37377713|gb|CF625486.1|CF625486

EST:     CCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: CCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|76933772|gb|DV173582.1|DV173582

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

EST: gi|211135879|gb|FL064022.1|FL064022

EST:     TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAG                         ATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC
genomic: TCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGAC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 39

GCACCTCGATGCCCCCTTATTCGGCGCCGATCTCATCCCCTCCGGCCGCCTCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcata ... gtatttgcagATAAGGATGTTTCAGACAAGAGTGTTACTGGGAGGAATGAACATGTATGACCGCTACCAGGATTGGCGCCTTGACGTTGATAACATGACATACGAG

Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 29

Block sizes: 47 (upstream exon), 45 (downstream exon)
Score: 35

Block sizes: 30 (upstream exon), 46 (downstream exon)
Score: 31

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GCACCTCGATGCCCCCTTATTCGGCGCCGATCTCATCCCCTCCGGCCGCCTCCGCCGGATGCGCGGGTGGCGCCACTCCCCCGGAGGACTCGAGGAAGAGgtacggcataccattgcattgcattgcaaagagacagacggttgctcttcgccatctcgcttgcttacacacatcaaccgcatcgatccatggcctgcgg

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA