ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CGAGTCCCTGGTCCGCCAGGCGGTGGCCAGCGCGTTCGCCAACGACTCCGGCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatacatggatctgtttgggttccttctctgctacttatactaagagcatctccaacaacgtgacctataaatgctttataattttaaaataagt...

Basic information

species	Zea mays
transcript	GRMZM2G107228_T01
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G107228_T01 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os01g22336.1 (Oryza sativa), 5'ss of exon 1

CGAGTCCCTGGTCCGCCAGGCGGTGGCCAGCGCGTTCGCCAACGACTCCGGCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatacatggatctgtttgggttccttctctgctacttatactaagagcatctccaa--caacgtgacctataaatgctttataattttaaaataagt---
|||| | |||||||| ||||| ||  ||| ||| ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||| ||| || | |     ||| | |   ||||||  |   |  |||   | ||    | | |||   |||  || |   | |        ||     || |  ||   
CGAGACGCTGGTCCGGCAGGCCGTCACCAACGCCTTCGCCAACGACTCCGGCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGAgtatgtttcctct--tctttctcttttcctttccaagt--ttagttttagtttttattcaaattaactagatcgtcagagaaagcggaagaa-gaattccgtggt

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211014736|gb|FL301860.1|FL301860

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|149080552|gb|EE169593.2|EE169593

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|211017511|gb|FL301866.1|FL301866

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCCTCCGCCAACAACACGGCGGAGCG
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACC-TCCGCCAACAACACGGCGGAGCG

EST: gi|149015485|gb|EE045362.2|EE045362

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|91878241|gb|EB408198.1|EB408198

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|76010677|gb|DT937847.1|DT937847

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|211014727|gb|FL301851.1|FL301851

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|211014731|gb|FL301855.1|FL301855

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|211011960|gb|FL301847.1|FL301847

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|211014725|gb|FL301849.1|FL301849

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|211017512|gb|FL301867.1|FL301867

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|211014738|gb|FL301862.1|FL301862

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

EST: gi|148941805|gb|EC886771.2|EC886771

EST:     GCATCGCCGCCGGCCTCATCCGCCTCCATTTCCACGACTGCTTCGTCAGG                         GGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT
genomic: GCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 34 (downstream exon)
Score: 8

CGAGTCCCTGGTCCGCCAGGCGGTGGCCAGCGCGTTCGCCAACGACTCCGGCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatac ... tggtgaacagGGCTGCGATGCGTCGGTGCTGCTGACCTCCGCCAACAACACGGCGGAGCGTGACGCGCCGCCCAACAACCCGAGCCTCCACGGGTTCCAGGTGATCGACG

Block sizes: 48 (upstream exon), 35 (downstream exon)
Score: 22

Block sizes: 43 (upstream exon), 48 (downstream exon)
Score: 26

Block sizes: 44 (upstream exon), 43 (downstream exon)
Score: 6

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CGAGTCCCTGGTCCGCCAGGCGGTGGCCAGCGCGTTCGCCAACGACTCCGGCATCGCCGCCGGCCTCATCCGCCTCCTTTTCCACGACTGCTTCGTCAGGgtacgtatacatggatctgtttgggttccttctctgctacttatactaagagcatctccaacaacgtgacctataaatgctttataattttaaaataagt

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aaaataag