ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GGCTGCAGTGAGCAAGAACATGGTGGAGAGTCTGGCAGTGCCAGCATTCCGCGTTGGGTACCCCATTATTACTGATAAACTCGACGAGCTGTATGAAAAGgtaaggatttgaagcagggcaccttgaggctcctttgttttccttagcctgcttcctgattactaattagactgatttgattttgacag

Basic information

species	Zea mays
transcript	GRMZM2G058702_T01
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G058702_T01 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os08g33440.1 (Oryza sativa), 5'ss of exon 1

GGCTGCAGTGAGCAAGAACATGGTGGAGAGTCTGGCAGTGCCAGCATTCCGCGTTGGGTACCCCATTATTACTGATAAACTCGACGAGCTGTATGAAAAGgta------------aggatttgaagcagggcaccttgaggctcctttg----ttttccttagcctgct--tcctgattactaattagactgatttgattttgacag
|||||||||||||||||||||||||||||| || || ||||| |||||||| || || ||||| ||| | || ||||| ||||| ||||| ||||| |||||             ||  ||||  |  ||      | |  ||||| ||    |||     |   || |  |  || | | |  |  |  ||||||| ||| |  ||
GGCTGCAGTGAGCAAGAACATGGTGGAGAGCCTCGCGGTGCCCGCATTCCGTGTGGGATACCCAATTGTGACCGATAAGCTCGATGAGCTATATGAGAAGgtgctatacctttggagactttgggggtggcagtgcttaacctcctgtgatggtttattacattttggtcattttgttgattgttggggttgatttggtttggttag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|67018197|gb|CO446946.1|CO446946

EST:     TGTATGAAAAG                         GTTAAGCCAAAGGGACTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC
genomic: TGTATGAAAAGgtaaggattt ... attttgacagGTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC

EST: gi|78027609|gb|DV495996.1|DV495996

EST:     ACGAGCTGTATGAAAAG                         GTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC
genomic: ACGAGCTGTATGAAAAGgtaaggattt ... attttgacagGTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC

EST: gi|15053899|gb|BI359444.1|BI359444

EST:     GCGTTGGGTACCCCATTATTACTGATAAACTCGACGAGCTGTATGAAAAG                         GTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC
genomic: GCGTTGGGTACCCCATTATTACTGATAAACTCGACGAGCTGTATGAAAAGgtaaggattt ... attttgacagGTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC

EST: gi|32939307|gb|CF044126.1|CF044126

EST:     CCATTATTACTGATAAACTCGACGAGCTGTA-GAAAAG                         G-TAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC
genomic: CCATTATTACTGATAAACTCGACGAGCTGTATGAAAAGgtaaggattt ... attttgacagGTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC

EST: gi|32942394|gb|CF047213.1|CF047213

EST:     ACCCCATTATTACTGATAAACTCGACGAGCTGTATGAAAAG                         GTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCGGCC
genomic: ACCCCATTATTACTGATAAACTCGACGAGCTGTATGAAAAGgtaaggattt ... attttgacagGTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 59

GGCTGCAGTGAGCAAGAACATGGTGGAGAGTCTGGCAGTGCCAGCATTCCGCGTTGGGTACCCCATTATTACTGATAAACTCGACGAGCTGTATGAAAAGgtaaggattt ... attttgacagGTTAAGCCAAAGGGAGTGACAATGACAGTGCTGCTGGCGAAGGCTGCAGCCATGGCTCTTGCACAGCACCCTGTTGTCAATGCTAGCTGCAGGGATGGAA

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 77

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 37