ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CCAAGGACCTGCAGGAGTACTTCAAGAAGAAGAAGCTGGAGGAGGCCGACCAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgcccccctgcaagatgcatcaaatttgtgaaccagaaagccttttgaataatccaatatggttcttcagatttttcctagtgccttcaaaca...

Basic information

species	Zea mays
transcript	GRMZM2G019807_T01
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G019807_T01 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os01g40710.1 (Oryza sativa), 5'ss of exon 1

CCAAGGACCTGCAGGAGTACTTCAAGAAGAAGAAGCTGGAGGAGGCCGACCAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgcccccctgcaagatgcatcaaatttgtgaaccagaaagccttttgaataatccaatatggttcttcagatttttcctagtgccttcaaaca--
||||||| |||||||| ||||||||| ||||||||||||| ||||| ||||||||||| ||||| |||||||||  |||||||||||||||||| |||||||||||||||||  || |       |  | |||   |||| |    | |  |  || ||       || || |    ||  ||  |||    |  |  |   
CCAAGGATCTGCAGGATTACTTCAAGCAGAAGAAGCTGGACGAGGCTGACCAGGGTCCCTTCTTTGGGTTCTTGGGCAAGAATGAGATTTCCAATGGAAGgtacgtttgccctgctccccccctgaaaagattcaggaacaaatttgtcaatgaaaaaaattctcttgattatctgcatgattgttagaaatt--aggcgat

upper sequence: GRMZM2G019807_T01 (Zea mays), 5'ss of exon 1
lower sequence: GLYMA20G30940.1 (Glycine max), 5'ss of exon 1

CCAAGGACCTGCAGGAGTACTTCAAGAAGAAGAAGCTGGAGGAGGCCGACCAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacg----tttgcccccctgcaagatgcatcaaatttgtgaaccagaaagccttttgaataatccaatatggttcttcagatttttccta---gtgccttcaaaca
| ||||| |||||||||||||||||  ||||||||||||| || ||  | |||||||| ||||| || ||| |   ||| |||||||||  ||||||||||||      ||      | |    ||   || ||| |||       ||     | ||          || | |   ||    |||||  ||   ||   |      |
CTAAGGAGCTGCAGGAGTACTTCAAACAGAAGAAGCTGGAAGATGCAAATCAGGGTCCTTTCTTTGGCTTCATTGGCAAAAATGAGATTAGCAACGGAAGgtaaaaaaattaattttcttttgggacctattaaagttg-------gagtttttcttatgcgggtttatgttgcatttttttttttttttaaatgttggtcttggta

upper sequence: GRMZM2G019807_T01 (Zea mays), 5'ss of exon 1
lower sequence: Vv18s0072g00120.t01 (Vitis vinifera), 5'ss of exon 1

CCAAGGACCTGCAGGAGTACTTCAAGAAGAAGAAGCTGGAGGAGGCCGACCAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtt---tgcccccctgcaagatgcatcaaatttgtgaaccagaaagccttttgaataatccaatatggttcttcagatttttcctagtgccttcaaaca
| ||||| || ||||| || |||||| ||| |||||| ||||||||| | |||||||| ||||| || ||| |   |||||||||||||||||| ||||||||| |    |    |  |      |   ||| |||| ||||         |||| |  |     ||| |||    |  || |   ||   ||  |  ||   
CTAAGGAGCTTCAGGATTATTTCAAGCAGAGGAAGCTTGAGGAGGCCAATCAGGGTCCTTTCTTTGGCTTCATTGGCAAGAATGAGATTTCCAATGGAAGgtacatgatgtttttctttctctattttgtcagattt-tgaattttttcagctttgggtttcaagaatttggccaatttgactgacccatatgagtataat--

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|60348627|gb|DN215600.1|DN215600

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|211181636|gb|FL239154.1|FL239154

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|5791019|gb|AI977811.1|AI977811

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|211181641|gb|FL239159.1|FL239159

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|15080497|gb|BI389084.1|BI389084

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|211184048|gb|FL239171.1|FL239171

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCTAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTANGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|211018456|gb|FL453048.1|FL453048

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|211181642|gb|FL239160.1|FL239160

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|211184050|gb|FL239173.1|FL239173

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTC-AACGGAAG                         GTGGGCCATGGTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|78022533|gb|DV490920.1|DV490920

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

EST: gi|15199059|gb|BI423633.1|BI423633

EST:     CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG
genomic: CAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 34 (upstream exon), 16 (downstream exon)
Score: 1

CCAAGGACCTGCAGGAGTACTTCAAGAAGAAGAAGCTGGAGGAGGCCGACCAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgc ... catcttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTATGCAACAGGCTCGGATTTTGTTCAGCAAATGAAGATCCTTCTCTCCAATTTTGGAATT

Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 64

Block sizes: 24 (upstream exon), 38 (downstream exon)
Score: 4

Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 63

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CCAAGGACCTGCAGGAGTACTTCAAGAAGAAGAAGCTGGAGGAGGCCGACCAGGGTCCGTTCTTCGGGTTCTTGCCCAAGAATGAGATTTCCAACGGAAGgtacgtttgcccccctgcaagatgcatcaaatttgtgaaccagaaagccttttgaataatccaatatggttcttcagatttttcctagtgccttcaaaca

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -cccccct