ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

TTTGTATCAGAATATTCACATGCCAGTGTTACTAATACTTGTCAGTTGGGAAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttcaatttttaatgttacttctatctgttcaatccgtgggtctttttggtgttataattttatgagtagatttaaaagactagaatgcttaga...

Basic information

species	Zea mays
transcript	AC217665.3_FGT005
intron #	1
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: AC217665.3_FGT005 (Zea mays), 5'ss of exon 1
lower sequence: LOC_Os02g35130.2 (Oryza sativa), 5'ss of exon 2

TTTGTATCAGAATATTCACATGCCAGTGTTACTAATACTTGTCAGTTGGGAAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaag---acttcaatttttaatgttacttctatctgttcaatccgtgg-gtctttttggtgttataattttatgagtagatttaaaagacta--gaatgcttaga
||||||||||| || || ||||| ||||| || | ||||||||| ||||  |||||  | || ||||| ||||| ||| |||||||||||||||| |||||||||    | ||||||| |  ||||    |  | ||||   || |||  | || ||   |||| | | |  ||    | | ||   |  ||   | ||| |    
TTTGTATCAGAGTACTCCCATGCTAGTGTGACCAGTACTTGTCAATTGGCCAGCACCGCGCTAAAGGATGGCCGTACCCTCAATGTTATTGATACGCCAGgtaagcatgcatcaatttattctgttctgaccttttgttt--tctgtgatgactattcattgttgttactacatattcatacttgttaatcttgtggatgtt----

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|61236534|gb|DN586284.1|DN586284

EST:     AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA
genomic: AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA

EST: gi|108823867|gb|EC277875.1|EC277875

EST:     AAGCACTACCCCGAAGGACGGCCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA
genomic: AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA

EST: gi|211058488|gb|FL335139.1|FL335139

EST:     AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCG
genomic: AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCG

EST: gi|61118680|gb|DN559641.1|DN559641

EST:     AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA
genomic: AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA

EST: gi|76294445|gb|DV034013.1|DV034013

EST:     AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA
genomic: AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA

EST: gi|6095394|gb|AW120061.1|AW120061

EST:     AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCGGACGAGGCTGGNAAAGAGATTGTTA
genomic: AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA

EST: gi|211304180|gb|FL452361.1|FL452361

EST:     CCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA
genomic: CCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA

EST: gi|24764590|gb|CA399754.1|CA399754

EST:     AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAG                         GGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA
genomic: AAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 180

TTTGTATCAGAATATTCACATGCCAGTGTTACTAATACTTGTCAGTTGGGAAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttc ... cttcctatagGGTTATTTGATATGAGCATTACATCGGACGAGGCTGGTAAAGAGATTGTTAAATGCATGAACATGGCTAAAGATGGGATACATGCAGTTCTGATGGTTTT

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 344

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 426

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 458

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TTTGTATCAGAATATTCACATGCCAGTGTTACTAATACTTGTCAGTTGGGAAGCACTACCCTGAAGGACGGCCGCACCATCAATGTTATTGATACACCAGgtaagacttcaatttttaatgttacttctatctgttcaatccgtgggtctttttggtgttataattttatgagtagatttaaaagactagaatgcttaga

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG