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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaaccttccaaatgaaattatttttggaagaaaaccatagtatcaggacataaacaggaatgtttgatccattggatgctcttacacaatcaagga...

Basic information

species Vitis vinifera
transcript Vv15s0046g03520.t01
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: Vv15s0046g03520.t01 (Vitis vinifera), 5'ss of exon 5
lower sequence: GLYMA09G39800.1 (Glycine max), 5'ss of exon 6
AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct--tccaaatgaaatt--atttttggaagaaaaccatagtatcaggacata--aacaggaatgtttgatccattggatgctcttacacaatcaagga
||||||||| |||| ||||| ||||||||||| ||||||||  | |  | | | |   |||  |||| |   |   |      |    ||  |||   |||   |   |   ||   || |  | |  |||| | ||     
AGCTTGAAGTCATTCCTTGGTGAAGAAAAGCTCAAGgtaaggccttatttccattcctattcaatttatcctacttatttgaggctgaagtgcatgctaactacatcatgacattgttttgg-gttgctacatattc-----

upper sequence: Vv15s0046g03520.t01 (Vitis vinifera), 5'ss of exon 5
lower sequence: GLYMA18G46390.1 (Glycine max), 5'ss of exon 6
-AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct--------tccaaatgaaattatttttggaagaaaaccatagtatcaggacataaacaggaatgtttgatccattggatgctcttacacaatcaagga
 ||||||||| |||| ||||| ||||||||||| ||||||||  | |        ||| ||| || | ||  |    |    |  |  | | | | |     ||  |||  |   ||   || |  | |  |||| |        
GAGCTTGAAGTCATTCCTTGGTGAAGAAAAGCTCAAggtaaggccttatttctaatcctaatcaattcatcctacctatttgag-actgaagctgcatgctgactagaacctaatattgttttggggttgctacata--------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|110415834|gb|EC981772.1|EC981772
EST:     GAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: GAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351037054|gb|FQ465046.1|FQ465046
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|30255051|gb|CB971498.1|CB971498
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110701264|gb|EE078642.1|EE078642
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351014077|gb|FQ434229.1|FQ434229
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|71870941|gb|DT019996.1|DT019996
EST:     GGCACCCAGAGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: GGCACCCAGAGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|22015208|gb|BQ800242.1|BQ800242
EST:     AGCTTGAAGACATTTC-TGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351029490|gb|FQ436884.1|FQ436884
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110722626|gb|EE099031.1|EE099031
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110689404|gb|EE065600.1|EE065600
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|294966590|gb|GW837725.1|GW837725
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351052425|gb|FQ452458.1|FQ452458
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110369745|gb|EC933913.1|EC933913
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCCGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|30323265|gb|CD006527.1|CD006527
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|30296186|gb|CB972980.1|CB972980
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCCGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110374344|gb|EC939025.1|EC939025
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|349826399|gb|FQ417896.1|FQ417896
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|349822047|gb|FQ408376.1|FQ408376
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|30253357|gb|CB970908.1|CB970908
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351056098|gb|FQ453560.1|FQ453560
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|349808339|gb|FQ405483.1|FQ405483
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|30254481|gb|CB971226.1|CB971226
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|156736254|gb|EV240375.1|EV240375
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110388071|gb|EC952787.1|EC952787
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|349824204|gb|FQ417591.1|FQ417591
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|37189632|gb|CF608981.1|CF608981
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351019470|gb|FQ429605.1|FQ429605
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|27579428|gb|CB002123.1|CB002123
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|33408487|gb|CF214114.1|CF214114
EST:     GAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCCGACTTGGATATCCCAATA
genomic: GAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|27580564|gb|CB003259.1|CB003259
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110384439|gb|EC948876.1|EC948876
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCCGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351053383|gb|FQ455719.1|FQ455719
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|77584263|gb|DV221204.1|DV221204
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110708707|gb|EE086556.1|EE086556
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|351019263|gb|FQ429423.1|FQ429423
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|71867285|gb|DT016340.1|DT016340
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|349833445|gb|FQ420191.1|FQ420191
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|161717186|gb|FC064703.1|FC064703
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|33406828|gb|CF212455.1|CF212455
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCCGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|27579427|gb|CB002122.1|CB002122
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110400084|gb|EC966626.1|EC966626
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCCGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|33410547|gb|CF216174.1|CF216174
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCCGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
EST: gi|110707835|gb|EE084684.1|EE084684
EST:     AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAG                         GAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA
genomic: AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaacct ... gattgtgcagGAAATTATTAATTTCACACTTCATTATATTGCTGACTTGGATATCCCAATA

Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AGCTTGAAGACATTTCTTGGAGAAGAAAAGCTGAAGgtaagaaccttccaaatgaaattatttttggaagaaaaccatagtatcaggacataaacaggaatgtttgatccattggatgctcttacacaatcaagga

-  TTGAAG
- - TGAAGA
- - - - - - - - TTGGAG
- - - - - - - -  TGGAGA
- - - - - - - - - GGAGAA
- - - - - - - - - - - AAGAAA
- - - - - - - - - - - - -  AAGCTG
- - - - - - - - - - - - - - AGCTGA
- - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aatgaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aagaaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -caggaca
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aacagga