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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TCCGGAGAACAATGAAAAACCAATGGAACAGAAGAGCAAGGTTAAGTTTAAAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcgtaccttccttgctggctacttctaattcttgttatgttgtcttatataaccacatctttgtagacatgttctgcaattttttgatgttgg...

Basic information

species Vitis vinifera
transcript Vv00s0203g00040.t01
intron # 4
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: Vv00s0203g00040.t01 (Vitis vinifera), 5'ss of exon 4
lower sequence: AT4G21450.1 (Arabidopsis thaliana), 5'ss of exon 4
TCCGGAGAACAATGAAAAACCAATGGAACAGAAGAGCAAGGTTAAGTTTAAAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttg-----cgtaccttccttgctggctacttctaattcttgttatgttgtcttatataaccacatctttgtagacatgttctgcaattttttgatgttgg
||| ||||| ||||| || |||||||| || | |||||  || |||||||||||||||||||| ||||||||||| |||||||||||||| |||||| ||||||||||     |  | |||   |  | |||  |  |    | ||  |       | | ||| ||  |||    |||| |  |     | ||     || |   
TCCTGAGAATAATGAGAAGCCAATGGATCAAAGGAGCAGAGTGAAGTTTAAAATCATGAGCCTGAAGGTGAAAGGTCCAATGGACTATGTGCCTGAGCTGgtatgttgatcttctgatcttatctattagctgatctttgaacatgaaaacagtagtaacatatccgaatccagataga-acataagata-ttgacccatct---

upper sequence: Vv00s0203g00040.t01 (Vitis vinifera), 5'ss of exon 4
lower sequence: AT4G05060.1 (Arabidopsis thaliana), 5'ss of exon 4
TCCGGAGAACAATGAAAAACCAATGGAACAGAAGAGCAAGGTTAAGTTTAAAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcgtaccttccttgctggctacttctaattcttgttatgttgtcttatataaccacatctttgtagacatgttctgcaattttttgatgttgg------------
||| ||||| |||||||| ||||||||||| ||||||  |||||||||||||||||||||| | ||| ||||||  ||||  |||||| | |||||| ||||||||     || |    |     ||  |   ||        |     |   ||| ||  | |  |||||       ||||   |  | | |||| |              
TCCTGAGAATAATGAAAAGCCAATGGAACAAAAGAGCGGGGTTAAGTTTAAAATCATGAGCTTGAAGATGAAAGTTCCAACAGACTATATGCCTGAGCTGgtatgtgaa--acttgtagtaactactggtcagaaagaaacagagaagataagataaaataagacttttgttcttggattctaagaagtgtggatgctcttgtgttttgcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|110724842|gb|EE101023.1|EE101023
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATAC
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATAC
EST: gi|27581899|gb|CB004594.1|CB004594
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|33403462|gb|CF209089.1|CF209089
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|26267184|gb|CA818247.1|CA818247
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110712576|gb|EE090353.1|EE090353
EST:     AAATCATGAGCCTAAAAGTGAAAGGACCCATGGACTATGTACCTGAAATG                         TTTGATGAACTGAAAGATCAAGTGGCAGT
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGT
EST: gi|32245411|gb|CD711230.1|CD711230
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|26265089|gb|CA816152.1|CA816152
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|30303167|gb|CB979961.1|CB979961
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110694278|gb|EE071243.1|EE071243
EST:     GACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: GACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|32245848|gb|CD711667.1|CD711667
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|349816674|gb|FQ404921.1|FQ404921
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|352768798|gb|FQ468010.1|FQ468010
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|27582368|gb|CB005063.1|CB005063
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|349817581|gb|FQ407371.1|FQ407371
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|32457525|gb|CD798699.1|CD798699
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110713187|gb|EE088888.1|EE088888
EST:     AATCATGAGCCTAAAGGTGAAAGGACCAATGGGACTATGTAC-TGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTANAACAGATACTGCNGGTTGTA
genomic: AATCATGAGCCTAAAGGTGAAAGGACCAATGG-ACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|33403546|gb|CF209173.1|CF209173
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110713517|gb|EE089505.1|EE089505
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|349811199|gb|FQ406187.1|FQ406187
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAACTGAAAGATCAAGTGGGCAGTAGAGCA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGG-CAGTAGAGCA
EST: gi|33407776|gb|CF213403.1|CF213403
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110374705|gb|EC939628.1|EC939628
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|349827283|gb|FQ421556.1|FQ421556
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGGTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|22011514|gb|BQ796548.1|BQ796548
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|352768232|gb|FQ469407.1|FQ469407
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110711188|gb|EE087431.1|EE087431
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTACGTACCTGAAATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTA
EST: gi|110360356|gb|EC923388.1|EC923388
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110711976|gb|EE089073.1|EE089073
EST:     ATCATGAGCCCTAA-GGTGAAAAGGAC-AATGGACTATGTACCTGANATG                         TTTGATGAGCTGAAA-ATCAGGTGGCA
genomic: ATCATGAGCC-TAAAGGTGAAA-GGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCA
EST: gi|349815417|gb|FQ400572.1|FQ400572
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACTTGAAATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGA
EST: gi|351053953|gb|FQ441719.1|FQ441719
EST:     AAATCATGAGCCTAAAGGTGAAATGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCACGTGGCAGTAAAGCAGA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGA
EST: gi|351033212|gb|FQ461984.1|FQ461984
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|349822581|gb|FQ412845.1|FQ412845
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|351028494|gb|FQ444775.1|FQ444775
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGG
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGG
EST: gi|30323727|gb|CD006989.1|CD006989
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110370543|gb|EC929968.1|EC929968
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|349812778|gb|FQ410420.1|FQ410420
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110711176|gb|EE087405.1|EE087405
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCNATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAAAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110383168|gb|EC947523.1|EC947523
EST:     AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
EST: gi|110720458|gb|EE096400.1|EE096400
EST:     AATCATGAGCCCTAAAG-TGAAAGGACCAATGGACTATGTACCTGAAATG                         TTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA
genomic: AATCATGAGCC-TAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcg ... cttgttgcagTTTGATGAGCTGAAAGATCAGGTGGCAGTAGAGCAGATACTGCGGGTTGTA

Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TCCGGAGAACAATGAAAAACCAATGGAACAGAAGAGCAAGGTTAAGTTTAAAATCATGAGCCTAAAGGTGAAAGGACCAATGGACTATGTACCTGAGATGgtatgttgcgtaccttccttgctggctacttctaattcttgttatgttgtcttatataaccacatctttgtagacatgttctgcaattttttgatgttgg

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGATG