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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTTTTGGGGCAGTCGTTGGGGAGGATTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtgtgggttgtgttgattgtgttacacttgttgctctgtttggttggtgagaattggctgcaaggaaagtatattattgacacagaaaactga...

Basic information

species Vitis vinifera
transcript Vv01s0146g00310.t01
intron # 3
splice site 5'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|351043256|gb|FQ443977.1|FQ443977
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATAAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351034494|gb|FQ448170.1|FQ448170
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351023552|gb|FQ432935.1|FQ432935
EST:     TTAAGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349817767|gb|FQ407756.1|FQ407756
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351030938|gb|FQ438974.1|FQ438974
EST:     TTAGGCCTGCGGTCTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|161721102|gb|FC070019.1|FC070019
EST:     TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349814730|gb|FQ409953.1|FQ409953
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351029889|gb|FQ427729.1|FQ427729
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351027179|gb|FQ440047.1|FQ440047
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349817147|gb|FQ405152.1|FQ405152
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110365214|gb|EC927841.1|EC927841
EST:     TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349828778|gb|FQ418513.1|FQ418513
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351017574|gb|FQ435927.1|FQ435927
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110685369|gb|EE062030.1|EE062030
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|71863160|gb|DT012215.1|DT012215
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110365136|gb|EC928913.1|EC928913
EST:     TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|71863702|gb|DT012757.1|DT012757
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110711640|gb|EE088353.1|EE088353
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349832945|gb|FQ423067.1|FQ423067
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349818046|gb|FQ408821.1|FQ408821
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349830010|gb|FQ420270.1|FQ420270
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349830031|gb|FQ420291.1|FQ420291
EST:     TTAGGCCTGCGGTCTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351015619|gb|FQ427104.1|FQ427104
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351028389|gb|FQ427285.1|FQ427285
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110719438|gb|EE094576.1|EE094576
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349826357|gb|FQ417854.1|FQ417854
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349815315|gb|FQ400470.1|FQ400470
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110711678|gb|EE088431.1|EE088431
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|161715045|gb|FC064252.1|FC064252
EST:     TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351014795|gb|FQ437995.1|FQ437995
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|294966748|gb|GW837767.1|GW837767
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|71861175|gb|DT010230.1|DT010230
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351027102|gb|FQ439963.1|FQ439963
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|351029935|gb|FQ427775.1|FQ427775
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|349812258|gb|FQ411853.1|FQ411853
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110398758|gb|EC965232.1|EC965232
EST:     TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110718337|gb|EE095058.1|EE095058
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|161716774|gb|FC067499.1|FC067499
EST:     TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|110702444|gb|EE081168.1|EE081168
EST:     TTAGGCCTGCGGTGTCGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
EST: gi|161710596|gb|FC058147.1|FC058147
EST:     TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAG                         ATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA
genomic: TTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtg ... ttttgcacagATGACAGTGAGGGATGCTCTAAACTCTGCACTTGATGAGGAAATGTCCGCA

Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTTTTGGGGCAGTCGTTGGGGAGGATTAGGCCTGCGGTGTGGGCATTGAGGAACTACTCGTCTGCGGAAAAACAGgtgagaagtgtgggttgtgttgattgtgttacacttgttgctctgtttggttggtgagaattggctgcaaggaaagtatattattgacacagaaaactga

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - gtgagaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aggaaag
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - acagaaa