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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AATACCTAAGGTTTTTGAGGAAGCGGATGAACACCAAACCCAGCAAGGGACCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctacttagcatttctccgttgatgagtacgtttgtttgccaatgcttgacactcacttgtcggtgaccatggtgttttagggtttctaacgtta...

Basic information

species Physcomitrella patens
transcript PP1S75_223V6.1
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S75_223V6.1 (Physcomitrella patens), 5'ss of exon 2
lower sequence: EFJ34798 (Selaginella moellendorffii), 5'ss of exon 2
AATACCTAAGGTTTTTGAGGAAGCGGATGAACACCAAACCCAGCAAGGGACCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctacttagcatttctccgttgatgagtacgtttgtttgccaatgcttgacactcacttgtcggtgaccatggtgttttagggtttctaacgtta
| ||||| |||||| |||||||||| |||||||| || ||||||||||| || || ||||||||  ||||||| || || || |||||||| ||| | ||||| |   | ||    |  ||  | |   ||| ||  |  |||    ||| ||  ||                                           
AGTACCTGAGGTTTCTGAGGAAGCGCATGAACACGAAGCCCAGCAAGGGGCCGATCCATTTTCGTGCTCCTGCCCGCATTCTCTGGAGGACGATCAGAGGgtaagag-atttcttttgccttggatctggagcac--tcttttctttatgttttccag------------------------------------------

upper sequence: PP1S75_223V6.1 (Physcomitrella patens), 5'ss of exon 2
lower sequence: EFJ23532 (Selaginella moellendorffii), 5'ss of exon 2
AATACCTAAGGTTTTTGAGGAAGCGGATGAACACCAAACCCAGCAAGGGACCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctacttagcatttctccgttgatgagtacgtttgtttgccaatgcttgacactcacttgtcggtgaccatggtgttttagggtttctaacgtta
| ||||| |||||| |||||||||| ||||||||||| ||||||||||| || || ||||||||  ||||||| || || || |||||||| ||| | ||||| |   | ||    |  ||  | |    |  ||  |  |||    ||| ||  ||                                           
AGTACCTGAGGTTTCTGAGGAAGCGCATGAACACCAAGCCCAGCAAGGGGCCGATCCATTTTCGTGCTCCTGCCCGCATTCTCTGGAGGACGATCAGAGGgtaagag-atttcttttgccttggatctcaatcac--tcttttctttatgttttccag------------------------------------------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|18325923|gb|BJ157922.1|BJ157922
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|37840797|gb|BJ598805.1|BJ598805
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67578235|gb|BJ950402.1|BJ950402
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|18353312|gb|BJ185367.1|BJ185367
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67572139|gb|BJ944963.1|BJ944963
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67691747|gb|BJ951980.1|BJ951980
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162249129|gb|FC420380.1|FC420380
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162184188|gb|FC359649.1|FC359649
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67694629|gb|BJ954862.1|BJ954862
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|37821428|gb|BJ579494.1|BJ579494
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162184189|gb|FC359650.1|FC359650
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67700129|gb|BJ960362.1|BJ960362
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67704631|gb|BJ964864.1|BJ964864
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGGCCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|100355108|gb|BY965008.1|BY965008
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162262069|gb|FC436850.1|FC436850
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162267118|gb|FC443349.1|FC443349
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67571310|gb|BJ944134.1|BJ944134
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|18363651|gb|BJ195723.1|BJ195723
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67716345|gb|BJ966605.1|BJ966605
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTTAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162249130|gb|FC420381.1|FC420381
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162246156|gb|FC423592.1|FC423592
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67694095|gb|BJ954328.1|BJ954328
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67507712|gb|BJ940592.1|BJ940592
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67701102|gb|BJ961335.1|BJ961335
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67695506|gb|BJ955739.1|BJ955739
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|37834782|gb|BJ592794.1|BJ592794
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|18342311|gb|BJ174346.1|BJ174346
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67570797|gb|BJ943621.1|BJ943621
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67724859|gb|BJ975118.1|BJ975118
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTTAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162246155|gb|FC423591.1|FC423591
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162277320|gb|FC453126.1|FC453126
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162221684|gb|FC397909.1|FC397909
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162277321|gb|FC453127.1|FC453127
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|162221683|gb|FC397908.1|FC397908
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
EST: gi|67508528|gb|BJ941392.1|BJ941392
EST:     CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGG                         AATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA
genomic: CCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 34 (upstream exon), 35 (downstream exon)
Score: 40

AATACCTAAGGTTTTTGAGGAAGCGGATGAACACCAAACCCAGCAAGGGACCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctac ... gttgttgcagAATGATCCCACACAAGACCAAAAGAGGAGCTGCCGCCCTTGAGCGCCTGAAGGCCTTTGAAGGAGTACCTGCACCTTACGATAAAATGAAGAGGATGGTC




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AATACCTAAGGTTTTTGAGGAAGCGGATGAACACCAAACCCAGCAAGGGACCCATTCATTTTCGATCTCCTGCTCGTATCCTGTGGAGGACTATCCGCGGgtacgcctacttagcatttctccgttgatgagtacgtttgtttgccaatgcttgacactcacttgtcggtgaccatggtgttttagggtttctaacgtta

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGGA