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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TGCCCCGAAAACTTCTGTTGCCTCTGCGCACCGTGATATGCGGGTGCGAGCGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtgaaattctctgctgattttggaaatgatgttgctgagagatagcactcggattttgattttgttttttgtttttttttttccgctgcttttt...

Basic information

species Physcomitrella patens
transcript PP1S227_66V6.1
intron # 2
splice site 5'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|162278618|gb|FC455016.1|FC455016
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|67703091|gb|BJ963324.1|BJ963324
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAAAAGATTCTGGCAAATGCGTCTGA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGA
EST: gi|100440503|gb|BY962232.1|BY962232
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|208389895|gb|DC937726.1|DC937726
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|67717186|gb|BJ967445.1|BJ967445
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100388007|gb|BY988480.1|BY988480
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|18364790|gb|BJ196863.1|BJ196863
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100444345|gb|BY982374.1|BY982374
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100361431|gb|BY965837.1|BY965837
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100364743|gb|BY947886.1|BY947886
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100362006|gb|BY947736.1|BY947736
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100428300|gb|BY977734.1|BY977734
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100438751|gb|BY961784.1|BY961784
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|67718911|gb|BJ969170.1|BJ969170
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGANAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100384785|gb|BY950775.1|BY950775
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100442174|gb|BY963067.1|BY963067
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|162158369|gb|FC334164.1|FC334164
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|162164820|gb|FC340516.1|FC340516
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100425091|gb|BY976968.1|BY976968
EST:     CGGTAACGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAAGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCATCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|162199219|gb|FC375055.1|FC375055
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|18361605|gb|BJ193671.1|BJ193671
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|208344467|gb|DC905028.1|DC905028
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100353093|gb|BY945602.1|BY945602
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|18360525|gb|BJ192588.1|BJ192588
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|208361763|gb|DC935003.1|DC935003
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|162198077|gb|FC372104.1|FC372104
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|18347134|gb|BJ179180.1|BJ179180
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100390972|gb|BY952982.1|BY952982
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|18363684|gb|BJ195756.1|BJ195756
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100406831|gb|BY955742.1|BY955742
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAN
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100362852|gb|BY947561.1|BY947561
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|18360168|gb|BJ192230.1|BJ192230
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|162163969|gb|FC339879.1|FC339879
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100385562|gb|BY969899.1|BY969899
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|162216862|gb|FC392212.1|FC392212
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|18327587|gb|BJ159590.1|BJ159590
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100449106|gb|CJ973405.1|CJ973405
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGANAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
EST: gi|100448912|gb|CJ973211.1|CJ973211
EST:     CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCT                         GTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA
genomic: CGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 35 (downstream exon)
Score: 15

TGCCCCGAAAACTTCTGTTGCCTCTGCGCACCGTGATATGCGGGTGCGAGCGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtga ... tgtgcttcagGTGAAACAGGCCATGACAGCGACGGAGAAGATTCTGGCAAATGCGTCTGAAAAGACTAAGCTTGCGCCTGGAGAGAATGTGTGGGTGAAGGCAGATGTTC




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TGCCCCGAAAACTTCTGTTGCCTCTGCGCACCGTGATATGCGGGTGCGAGCGGTAATGGCTCCCCAGAAGCAGGAGCGCTCTCCTGCCTCCACTGGAGCTgtaagtgtgaaattctctgctgattttggaaatgatgttgctgagagatagcactcggattttgattttgttttttgtttttttttttccgctgcttttt

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCT