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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CCCTTTCTCTGGTCGGCTCCCCTGCGTATGCCCAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgtgtgtgtattgcgtttttatcctcttttctcattgtactgcttgtgcttaagctcatatgacatggtttgtgtttttttagtctttctctt...

Basic information

species Physcomitrella patens
transcript PP1S215_81V6.1
intron # 1
splice site 5'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|162253990|gb|FC430221.1|FC430221
EST:     GCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18330539|gb|BJ162545.1|BJ162545
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATCCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162212757|gb|FC388557.1|FC388557
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162243512|gb|FC420632.1|FC420632
EST:     GCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162208358|gb|FC384670.1|FC384670
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|67691839|gb|BJ952072.1|BJ952072
EST:     GTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18336509|gb|BJ168531.1|BJ168531
EST:     GCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|19788983|gb|BQ041032.1|BQ041032
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162261494|gb|FC438330.1|FC438330
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|19785303|gb|BQ040298.1|BQ040298
EST:     CACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18328864|gb|BJ160870.1|BJ160870
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|37824978|gb|BJ583044.1|BJ583044
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18335100|gb|BJ167118.1|BJ167118
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|37844488|gb|BJ602496.1|BJ602496
EST:     AGCGCCAGCCCCACGGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: AGCGCCAGCCCCAC-GCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162263767|gb|FC439647.1|FC439647
EST:     GTCGTCCCTCCTCATCCACCATG                         TCGTTCATCGCGGGTGAGGAATTTCAGCACATTTTTCGTGTCCTTAACACG
genomic: GTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|67718206|gb|BJ968465.1|BJ968465
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18341523|gb|BJ173558.1|BJ173558
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18350522|gb|BJ182573.1|BJ182573
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18345865|gb|BJ177908.1|BJ177908
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18375221|gb|BJ206799.1|BJ206799
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|67691546|gb|BJ951779.1|BJ951779
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18328270|gb|BJ160274.1|BJ160274
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162263768|gb|FC439648.1|FC439648
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|67726692|gb|BJ976951.1|BJ976951
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|19790285|gb|BQ041800.1|BQ041800
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18337077|gb|BJ169101.1|BJ169101
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162248121|gb|FC420841.1|FC420841
EST:     GTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162261495|gb|FC438331.1|FC438331
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162210621|gb|FC386309.1|FC386309
EST:     GCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|18373983|gb|BJ205561.1|BJ205561
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|7147616|gb|AW509538.1|AW509538
EST:     GCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|19789538|gb|BQ041366.1|BQ041366
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|208387492|gb|DC933355.1|DC933355
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162264394|gb|FC440560.1|FC440560
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162248122|gb|FC420842.1|FC420842
EST:     GTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: GTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|37849277|gb|BJ607285.1|BJ607285
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|19790144|gb|BQ041716.1|BQ041716
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|67508331|gb|BJ941199.1|BJ941199
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|162199889|gb|FC376455.1|FC376455
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
EST: gi|208368935|gb|DC947493.1|DC947493
EST:     CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATG                         TCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG
genomic: CAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 35 (downstream exon)
Score: 276

CCCTTTCTCTGGTCGGCTCCCCTGCGTATGCCCAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgt ... tttttttcagTCGCTCATCGCGGGTGAGGAATTTCAGCACATTCTTCGTGTCCTTAACACGAACGTCGATGGACGCCAGAAGATCATGTTTGCCTTGACCTCCATTAAGG




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CCCTTTCTCTGGTCGGCTCCCCTGCGTATGCCCAGCGCCAGCCCCACGCTGTGCTTCTTGTCGTCCCTCCTCATCCACCATGgtgtgtgtgtgtgtgtattgcgtttttatcctcttttctcattgtactgcttgtgcttaagctcatatgacatggtttgtgtttttttagtctttctctt

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTCCTC