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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgatattaaaattcacgtaatttccagcaagctcctaccttttttatgggtttgcatgacttgcttcacatcacacgtgtcacag

Basic information

species Oryza sativa
transcript LOC_Os02g33230.1
intron # 8
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os02g33230.1 (Oryza sativa), 5'ss of exon 8
lower sequence: GRMZM2G026900_T02 (Zea mays), 5'ss of exon 8
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgatattaaaattcacgtaatttccagcaagctcctaccttttttatgggtttgcatgacttgcttcacatcacacgtgtcacag
|||||||||| |||||||| ||||||||||||||||||||||||||  ||||||| || || ||||||||||||||||||||  |||||||||||||||||| | |   | ||      | ||   ||| | | | ||| |||| |||   ||  |||| |      |  | |     |  ||||
AACCTAACCCTCACATGGGACATCTTGCATGGGCTGATGCTTTTGTCATAACAGCAGACTCGATAAGTATGTTAAGTGAGGCAGGTAGCACAGGgtaagctgttttcttatttggaacgactttt-gcatgttgcaaccatttt-atgaagttttatgagtgtggctagcttattatcgctacag

upper sequence: LOC_Os02g33230.1 (Oryza sativa), 5'ss of exon 8
lower sequence: GLYMA12G31170.1 (Glycine max), 5'ss of exon 8
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaag-ctgatattaaaattcacgtaatt---tccagcaagctcctaccttttttatgggtttgc-atgacttgcttcacatcacacgtgtcacag-----
|||| ||||| || ||||||||||| || ||||| ||||| |||||||| ||||| |||||  | || ||| ||||||| ||||| ||||| ||||| | |||  |  || |||    | |||   | |  |   |||  | ||||   |||  ||| |    | ||  |    ||             |     
AACCAAACCCACAAATGGGGCATCTAGCTTGGGCCGATGCATTTGTTGTCACAGCAGATTCAGTTAGCATGATAAGTGAAGCTTGCAGCACTGGgtatgtctgtcagaaatatttctatgattagattctccccactctgatctttaa-atgattttactgaaatttagtatttatagagtccactgatgcttct

upper sequence: LOC_Os02g33230.1 (Oryza sativa), 5'ss of exon 8
lower sequence: GLYMA13G39140.1 (Glycine max), 5'ss of exon 8
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaag-ctgatattaaaattcacgtaatttccagcaagctcctaccttttttatgggtttgcatgacttgcttcacatcacacgtgtcacag--------
|||| ||||| || ||||||||||| || ||||||||||| |||||||| ||||| |||||  | || ||| ||||||| ||||| ||||| ||||| | |||  |  |  |||    | |||         | |     | | | |  | |||   ||      |     | | |     ||| |        
AACCAAACCCACATATGGGGCATCTGGCTTGGGCTGATGCATTTGTTGTCACAGCAGATTCAGTTAGCATGATAAGTGAAGCTTGCAGCACCGGgtatgtctgtcaaaagtatttctatgattagatttcctcactctgatctctaaatgatttaactggaaatttggtttttatagagtccactgtttttttt

upper sequence: LOC_Os02g33230.1 (Oryza sativa), 5'ss of exon 8
lower sequence: AT5G22350.1 (Arabidopsis thaliana), 5'ss of exon 8
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgatattaaaattcacgtaatttccagcaagctcctaccttttttatgggtttgcatgacttgcttcacatcacacgtgtcacag
|||| || ||  ||||||||||||| || |||| |||||||||||| || ||||| |||||  | ||| || |||| || ||||||||||| |||||   | ||||    |   |     ||||   |   |  | || |    |||    ||      | | ||   |  ||            
AACCAAATCCTTACATGGGGCATCTAGCTTGGGGTGATGCTTTTGTGGTGACAGCAGATTCAGTCAGTCTGATAAGCGAAGCTTGTAGCACCGGgtata-taatatgctgacataaaccctttctttcttccaacaacttgaactataaccttagtactccttctctgctgcag-----------

upper sequence: LOC_Os02g33230.1 (Oryza sativa), 5'ss of exon 8
lower sequence: Vv08s0007g02890.t01 (Vitis vinifera), 5'ss of exon 9
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagct--gatattaaaattcacgtaatttccagcaa---gctcctaccttttttatgggtttgcatgacttgcttcacatcac-acgtgtcacag--------
|||| || || || ||||||||| |  | ||||||||||| |||||  | |||||||||||  | |||||||| |||||||||||||| || ||||| | |   ||| |    |||||    ||| ||       | |   |  | |||| | |  |    |   | | |||  ||||  || | |   |         
AACCGAATCCACATATGGGGCATTTGTCTTGGGCTGATGCCTTTGTCATCACAGCTGATTCAGTGAGTATGTTGAGTGAGGCTTGTAGTACTGGgtatgtttaaataatggttttcaccctctttgcaatggttggtttaaaagtattttttagactgaagtttatcggttcttatcattactttttttaattttgcag

upper sequence: LOC_Os02g33230.1 (Oryza sativa), 5'ss of exon 8
lower sequence: Vv06s0080g00830.t01 (Vitis vinifera), 5'ss of exon 8
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgatattaaaattcac----gtaatttccagcaagctcctaccttttttatgggtttgcatgacttgcttcacatcacacgtgtcacag--------------------------------------------------------
|||| || || |||||||| ||||| || |||||||||||||||||||| ||||| |||||  | |||||| ||||||||||||| ||||||||||| |||   |  | |||| ||     | |||      ||  |   | | | || |||  | |   |  |||  | |   |   |  ||  ||                                                          
AACCAAATCCACACATGGGACATCTAGCTTGGGCTGATGCTTTTGTTGTCACAGCAGATTCAGTGAGTATGATAAGTGAGGCTTGCAGCACAGGgtatgctt-cagaagaatttacccctatgattgatgataa-tttggagcatcttgatgt-tctctttttctttttccttttaggatatgatacttgtttatattctcttattatattgcaatattagttatttcaatttttgttgctgtag

upper sequence: LOC_Os02g33230.1 (Oryza sativa), 5'ss of exon 8
lower sequence: PP1S82_141V6.1 (Physcomitrella patens), 5'ss of exon 8
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgatattaaaattcacgtaatttccagcaagctcctaccttttttatgggtttgcatgacttgcttcacatcacacgtgtcacag-----------------------------------------------------------------------------------------
  || ||||||||| | || ||||| |||||||  ||||| |||||||| |||||||||||| | |||||||| || || || || ||||||||||| |    | ||  |||  |  |    |   |   |||  |    |  | ||    |   ||   | |         |  | |    | |                                                                                         
GTCCAAACCCGCACCTTGGACATCTCGCATGGGGGGATGCATTTGTTGTCACAGCTGATTCTGTTAGTATGTTGAGCGAAGCATGCAGCACAGGgtatgtgacttttttaatagaattggcatattgtgtgctgttcgtatcctcataacctcaaattgatggtggggttatatgcatagttctggttattaacaacaaatctttgacctagttgttacttttaaactcttgtaatcatttccagttttattgccaccggtctgctgtgaacag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|29660003|gb|CB656278.1|CB656278
EST:     GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGG                         GAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
genomic: GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgat ... cgtgtcacagGAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
EST: gi|88985474|gb|CI002991.1|CI002991
EST:     GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGG                         GAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
genomic: GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgat ... cgtgtcacagGAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
EST: gi|88906754|gb|CI375826.1|CI375826
EST:     GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGG                         GAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
genomic: GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgat ... cgtgtcacagGAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
EST: gi|86501379|gb|CI144012.1|CI144012
EST:     GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGG                         GAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
genomic: GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgat ... cgtgtcacagGAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
EST: gi|38472562|gb|CF956694.1|CF956694
EST:     GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGG                         GAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
genomic: GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgat ... cgtgtcacagGAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
EST: gi|29677033|gb|CB673308.1|CB673308
EST:     GTCGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGG                         GAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC
genomic: GTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgat ... cgtgtcacagGAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 75 (upstream exon), 74 (downstream exon)
Score: 13

AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgat ... cgtgtcacagGAAGCCTGTTTACGTCATTGGGACTGAGTATTGTAAATGGAAGTTTTCTGCTTTTCACAAGACTCTACGGGAGCGAGGAGTTGTCCGTCCTTTCACTGGA




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AACCTAACCCGCACATGGGGCATCTTGCATGGGCTGATGCTTTTGTTGTAACAGCTGATTCTATAAGTATGTTAAGTGAGGCTTGTAGCACAGGgtaagctgatattaaaattcacgtaatttccagcaagctcctaccttttttatgggtttgcatgacttgcttcacatcacacgtgtcacag

- - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGA
- - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGAT