Sequence
atgc intronic sequence ATGC exonic sequenceTTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa...
Basic information
species | Oryza sativa |
transcript | LOC_Os09g19700.1 |
intron # | 5 |
splice site | 5' |
intron type | U2 |
Orthologous splice sites
atgc intronic sequence ATGC exonic sequence
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GRMZM2G132184_T03 (Zea mays), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaataccgc-agtaacctccttaaatt-caaataa----
||||||||||| ||||| || ||||||||||| |||||||| || |||||||||||||||||||| ||||| |||||||| || || ||||| ||||| ||||||| | ||| | | ||| | || | || ||| || ||| | | | | || || ||| | | | | || |
TTTGGGCTTGCGAAAGACGGACCTGTAGGTGATAAAACTCATGTTTCTACTCGAGTAATGGGAACCTATGGATATGCAGCACCAGAATATGTCATGACTGgtaagtatcttgtca-tatttgccatgactgtgtccatcg-----aactctggtggtgagcaagtctggccactgccagtgctaccagtgagcaacgaaccattca
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GRMZM2G150806_T01 (Zea mays), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcag-tcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatata-aataccgcagtaacctccttaaattcaaataa
||||||||||| ||||| || ||||||||||| |||||||| || |||||||||||||||||||| ||||| |||||||| || || ||||| ||||| |||||| | | |||| | || | | | ||||| | | || | || |||| || || | || | || | || |
TTTGGGCTTGCAAAAGACGGACCTGTAGGTGATAAAACTCATGTCTCTACTCGAGTAATGGGAACCTATGGATATGCAGCACCAGAATATGTCATGACTGgtaagcacattgtcagattttctctaccttctttcttaccctctctt-tcggagtacaactgtaacatctgatcatgtgcgtacatactcct-tccacaccc
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA08G40770.1 (Glycine max), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagt---gcttcatcagtcatcaccagggttg-acgttaccgccggtaaccgcgggggtacggtaatata-aataccgcagtaacctccttaaattcaaataa
||||| ||||| |||||||| || | ||||| ||||| || |||||||||||||| |||||||| |||||||||||||| || || ||||| ||||| ||||||| | | | | | ||| | ||| | | | || | || | | ||||| | || | | | | | | |
TTTGGACTTGCAAAAGATGGTCCAGAGGGTGATAAAACCCATGTGTCTACTCGAGTGATGGGAACCTATGGTTATGCAGCACCAGAATATGTCATGACGGgtaagtttagttgggtatgctaaacccaagtttgcaaggttttgctgctatttatcag--tgaagtaatgcatgatcattctctggtatttccatctcccta---
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA19G02730.1 (Glycine max), 5'ss of exon 4
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcac-cagggttgacgttaccgccggtaaccgcggg-ggtacggtaatataaataccgcagtaacctccttaaattcaaataa
|||||||||||| |||||| || || || || |||||||| || || ||| ||||||||||||| | || |||||||| || |||||||| ||||| ||| ||| ||| |||| | ||| | | | || | | | | | | | || || | | | |
TTTGGGCTTGCCCAAGATGCTCCAGTGGGAGATAAAACTCATGTTTCCACTGAAGTAATGGGAACACAAGGCTATGCAGCCCCTGAGTATGTGATGACGGgttagtatgctctcaaacatctcattttgttcaaccaaatgttactattcatgtgtagcatacaatcacaagctttatgaaaatacctagctagttaggg--
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA19G02360.1 (Glycine max), 5'ss of exon 4
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtg------cttcatcagtcatcaccagggtt-gacgttaccgccggtaaccgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa
||||| |||||||||||||| |||| || || || || || || ||||| |||| |||||||||||||||||||| ||||| |||||||| ||||| ||| ||| |||| | | || | || || | ||| | | | || | | || ||| || | | ||||| |
TTTGGACTTGCCAAAGATGGTCCTGAGGGGGAGAAGACACATGTATCTACAAGAGTTATGGGAACATATGGTTATGCTGCTCCTGAGTATGTGATGACTGgtgagttttgagccttctttattccttttcaacttttaatgtttcttaaacttaatatataactaagac--tctagata--gctaaagttatttaaaatttgca---
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA09G37580.1 (Glycine max), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtg-cttcatcagtcatcaccagggttgacgttaccgccggtaacc--gcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa
||||| || ||||||||||| |||| ||| || || || ||| | || || |||| |||||||||||||||||||||||||| || ||||| ||||| ||| ||| | | | | | | || || | | | ||| || | | | || | | || |||
TTTGGACTCGCCAAAGATGGTCCTGAAGGGGAAAAGACACACATATCAACAAGAGTCATGGGAACATATGGTTATGCAGCTCCTGAATATGTGATGACTGgtgagtttcctttttaattttgtgtttcattaacttcagactttgcaacatgattcaggctctagattgcttatgacatctcggattgtgtacattattg---
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA18G49060.1 (Glycine max), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaag-tgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa-----
||||| || ||||||||||| |||| ||| || || || ||| | ||||| |||| |||||||||||||||||||| ||||| |||||||| ||||| ||| || | | | | | | | || || | | | | | || | || | || | || || | | | | || || ||| ||
TTTGGACTCGCCAAAGATGGTCCTGAAGGGGAAAAGACACACATATCTACCAGAGTCATGGGAACATATGGTTATGCTGCTCCTGAGTATGTCATGACTGgtgagattcctttttatttttgtgtttcattaacttca--gacttttactacatggttcaggc--tctagattgc-taatggcgtc-ttggatttgtgtatatttg
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA01G04930.1 (Glycine max), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagt---gcttcat-cagtcatcaccagggttgacgttaccgccggtaaccg--cgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa
||||| |||||||||||||| || | |||||| ||||| || ||||||||||| || |||||||| |||||||| ||||| || || ||||| ||||||||||||| | || || || || | | | | ||| | | | ||| | ||| | || | | | | || |
TTTGGTCTTGCCAAAGATGGTCCAGAAGGTGATAAAACCCATGTGTCTACTCGGGTGATGGGAACCTATGGTTACGCAGCACCAGAATATGTCATGACAGgtaagtttagtttgatatagccagttcaaatctgcatgttttagttgttttttattcggaaagtcacaaatgcatatgctttattgttatttctatctgt------
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA02G02570.1 (Glycine max), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagt---gcttcat-cagtcatcaccagggttgacgttaccgccggtaaccgcgggggta--cggtaatataaataccgcagtaacctccttaaattcaaataa
||||| |||||||||||||| || | |||||| ||||| || |||||||||||||| |||||||| |||||||||||||| || || ||||| ||||| ||||||| | || || || || | | | | ||| | | || || | | || || | | | || |
TTTGGTCTTGCCAAAGATGGTCCAGAAGGTGATAAAACCCATGTGTCTACTCGAGTGATGGGAACCTATGGTTATGCAGCACCAGAATATGTCATGACGGgtaagtttagtttgatatagccagttcaaatctgcatgttttagttgttatttatttggaaagtcacacatgcttatgctttgttgctgtttctatctgt------
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA18G16300.1 (Glycine max), 5'ss of exon 6
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa------
||||| ||||| |||||||| || | ||||| |||||||| |||||||||||||| |||||||| |||||||||||||| || || ||||| ||||| ||||||| || | | || | | | | || | | || | || | | || | ||| | | | || || | ||
TTTGGACTTGCAAAAGATGGTCCAGAGGGTGATAAAACTCATGTGTCTACTCGAGTGATGGGAACCTATGGTTATGCAGCACCAGAATATGTCATGACGGgtaagt--ttagttggctat-gctaagtccaagtttgcaggtt-tagctgctatttttcagtgaagtaatg--catgatcattctctggtatctgattacatattt
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA19G02470.1 (Glycine max), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcat-caccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaatac---cgcagtaacctccttaaattcaaataa
||||||||||| |||||| || | || || |||||||| || || ||| ||||||||||||| | || |||||||| || |||||||||||||||||| || | | ||| || | || ||| || | | | | ||||| || | | | | | || | |
TTTGGGCTTGCGCAAGATGCTCCAATGGGAGATAAAACTCATGTTTCCACTGAAGTAATGGGAACACAAGGCTATGCAGCCCCTGAGTATGTTATGACAGgttagcatatatactctcaaacatctcattttgttgaaccaaatgtttctattcatttgtagca-tataatcacaagctttatgaaatacctag-tccgggc--
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: AT3G01300.1 (Arabidopsis thaliana), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgct-tcatcagtcatcac-cagggttgacgttaccgccggtaaccgcgggggtacggt--aatataaataccgcagtaacctccttaaat---tcaaataa--------------------------------------------------------------------------------
||||||||||||||||||| |||| | | ||||||||| |||||||||||||| ||||| || |||||||| || || || |||||||| ||||| ||||||| | | | ||| | | || || | | || | | | || ||| |||||| | | || ||||| | | || | | ||
TTTGGGCTTGCCAAAGATGCTCCTGATGAAGGCAAAACTCATGTGTCTACTCGAGTCATGGGCACTTATGGTTACGCTGCCCCCGAGTATGTAATGACCGgtaagttttgttttttgtctttgcatttcattcactcctctacttatatcttctctg-tatggttgaatatagaaaat-tagaaacctacacatatggttgatgcaaagtattactatgatgaaacatacatctaatttctttggtttgagattatatataactactgtcctcttattactccttag
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: Vv14s0108g00380.t01 (Vitis vinifera), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaat-accgcagtaacctccttaaattcaaataa
||||| ||||||||||| || || | ||| || || || || || |||||||| |||||||| || ||||| |||||||| || |||||||| ||||||||| | | | | | | ||| | || ||| | | | || || | | | | | | | | | ||||| | | |
TTTGGACTTGCCAAAGACGGTCCAGAAGGAGATAAGACACATGTATCTACTCGTGTAATGGGCACCTATGGCTATGCAGCCCCTGAGTATGTGATGACAGgtgactaca-cttaaactctcattactatttttattatttttgacatttctgtggctatgctcttgtgacccatctaattctgctcctcctagttttgcca
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: PP1S31_254V6.1 (Physcomitrella patens), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaag-tgcttcatcagtcatcaccagggtt--gacgttaccgccgg-----taaccgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa--------------------------------------------
||||| || || |||||||| |||| ||| |||||||||||||| || || || || ||||| || ||||| ||||| || || |||||||| ||||||||| | | |||| | |||| || || | | | | ||| | ||| || || | | | | | | || ||
TTTGGCCTAGCGAAAGATGGGCCTGAAGGAGACAAAACTCACGTATCAACACGTGTCATGGGCACCTATGGCTATGCTGCCCCTGAGTATGTGATGACAGgtgtgattcttccctattcattcgtgtcttttcgaaaatgtattcaggcgctttgccgagtactcgcggaaacttatcttgtatatgcagcgatctgattttgtgtaggttcttcattcggttgcttcatttaactatgtattgatttgcag
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: PP1S207_2V6.1 (Physcomitrella patens), 5'ss of exon 5
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggta-accgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa
|| ||||| || |||||||| |||| ||| |||||||||||||| || || || || ||||| || ||||| ||||| || || |||||||| ||||| ||| | || | | | || | | || | | || || | | | | | | | | ||||||||||| |
TTCGGGCTAGCGAAAGATGGACCTGAAGGAGACAAAACTCACGTTTCAACACGTGTTATGGGCACCTATGGCTATGCTGCCCCTGAGTATGTGATGACTGgtgtgaactctcccccactctatcatg-tctactctgaaaatgctatgacgtcttttccagatcacttttcttttttacgtatgatcataaattcaaatga
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: EFJ35779 (Selaginella moellendorffii), 5'ss of exon 4
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa--------------
|| || | || || ||||| || | |||||||||||| ||||| || || || |||||||| || ||||| ||||||||||| || || || |||||||||| | | || | | | || | | | || | | | | || || | | | ||| | | | | |||
TTCGGTTTGGCAAAGGATGGTCCGGAAGGTGACAAAACGCACGTTTCGACGCGCGTAATGGGGACTTATGGCTATGCAGCTCCAGAATACGTAATGACAGgtactttttcttctcgttcttgctcgagtgaagctctctttcgtttcttcagtcgtttcgacgatct---tgcgagagtgagagcttgcgactcatgcctttccggctttgcag
upper sequence: LOC_Os09g19700.1 (Oryza sativa), 5'ss of exon 5
lower sequence: EFJ10730 (Selaginella moellendorffii), 5'ss of exon 4
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggta-cggtaatataaataccgcagtaacctccttaaattcaaataa--------------
|| || | || || ||||| || | |||||||||||| ||||| || || || |||||||| || ||||| ||||||||||| || || || |||||||||| | | || | | | || || | | | || | | | || || || | | | ||| | | | | |||
TTCGGTTTGGCAAAGGATGGTCCGGAAGGTGACAAAACGCACGTTTCGACGCGCGTAATGGGGACTTATGGCTATGCAGCTCCAGAATACGTAATGACAGgtactttttcttctcgttcttgctcgagt-gaagctgtctttcgtttcttcagccgtttcgacgatct---tgcgagagtgagagcttgcgactcatgcctttccggctttgcagMapped EST sequences
Showing partial alignments of ESTs and genomic sequences. See full alignments
ATGC EST sequence
ATGC genomic sequence (exon)
ATGC genomic sequence (truncated intron)
EST:
gi|3761134|gb|C98382.1|C98382EST: TCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAG GCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
genomic: TCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgctt ... cacttttcagGCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
EST:
gi|29644791|gb|CB649798.1|CB649798EST: CATATGGTTATGCAGCTCCGGAGTATGTTATGACAG GCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
genomic: CATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgctt ... cacttttcagGCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
EST:
gi|163929492|gb|EG709283.1|EG709283EST: TCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAG GCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
genomic: TCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgctt ... cacttttcagGCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
EST:
gi|8335359|gb|BE040343.1|BE040343EST: TCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAG GCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
genomic: TCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgctt ... cacttttcagGCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTG
RNA-Seq data
ATGC Supported by RNA-Seq exonic block that spans over the splice site
atgs Truncated intron sequence
Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.
Found data for 1 RNA-Seqlibrary.
Block sizes:
74 (upstream exon),
70 (downstream exon)
Score:
24TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgctt ... cacttttcagGCCATTTGACATCAAAGAGTGATGTCTATAGTTTTGGTGTGGTGCTACTTGAGATGATGTCAGGGCGCAGGTCAATGGACAAGAACCGCCCAAATGGTGA
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Putative cis-regulatory sequences
atgc | intron | ATGC | exonic elements by Pertea et al. |
ATGC | exon | atgc | putative intronic elements |
| | ATGC | putative exonic elements identified for retained introns |
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|
TTTGGGCTTGCCAAAGATGGCCCTGTAGGTGACAAAACTCACGTGTCTACTCGAGTAATGGGAACATATGGTTATGCAGCTCCGGAGTATGTTATGACAGgtaagtgcttcatcagtcatcaccagggttgacgttaccgccggtaaccgcgggggtacggtaatataaataccgcagtaacctccttaaattcaaataa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - cgccggt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gcggggg