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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AGGTCTTGCCTTTGCCCAGGAATCTGGATGTCTTTTTCTTGAGAGCAGTGCCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgcagaaataatatactaagagcaagtttaatagtatagcccactattagctccaattcatctatagtcaatctaatagccaattcatacaat...

Basic information

species Oryza sativa
transcript LOC_Os03g05280.1
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os03g05280.1 (Oryza sativa), 5'ss of exon 5
lower sequence: AC149818.2_FGT001 (Zea mays), 5'ss of exon 5
AGGTCTTGCCTTTGCCCAGGAATCTGGATGTCTTTTTCTTGAGAGCAGTGCCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgcagaaataatatactaagagcaagtttaatagtatagcccactattagctccaattcatctatagtcaatctaatagccaattcatacaat-------
||||||||| ||||| || |||  ||||||||| ||| | ||||| ||||| |||||||||||||| ||||| ||||| |||||||||||||| |||||||||    |||| |||  | | || |     ||  | || ||  || | |  ||    || ||   |||  | | || | ||   |     | || | |||       
AGGTCTTGCATTTGCACAAGAACGTGGATGTCTCTTTGTCGAGAGTAGTGCTAAAACAAGAGAAAACGTGGAAAAATGTTTTGAAGAGCTTGCATTAAAGgta---ctgcataaaccaga-acaatacttaatatcaaaagcttatctctttacc--cttcataccattca-aatctaccttccaatagctccagaaaattagattt

upper sequence: LOC_Os03g05280.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA03G34330.1 (Glycine max), 5'ss of exon 5
AGGTCTTGCCTTTGCCCAGGAATCTGGATGTCTTTTTCTTGAGAGCAGTGCCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtata-cttgcagaaat--aatatactaagagcaagtttaatagtatagcccactattagctccaattcatctatagtcaatctaatagccaattcatac--aat
 ||| | ||  ||||  | ||||  || ||| |  |||||||  | ||||| |||||||| |||||||||||| | |||||||| || |||||  |||||||| |  ||  |||| |    |  | |      | || |  ||  |||   || | ||    |||||| |   | |||      |  |   ||  ||  |  |||
GGGTTTAGCACTTGCTAAAGAATTGGGGTGTTTGCTTCTTGAATGTAGTGCTAAAACAAGGGAAAATGTGGAGCAGTGCTTTGAGGAACTTGCTCTAAAGgtacagtttttagaagtttgcttcagtgctgttatgtcttgta-cataagtcaatttt----ccaattaactgacagtagggtcatgaattaacccaagcctaat

upper sequence: LOC_Os03g05280.1 (Oryza sativa), 5'ss of exon 5
lower sequence: GLYMA19G37020.1 (Glycine max), 5'ss of exon 5
AGGTCTTGCCTTTGCCCAGGAATCTGGATGTCTTTTTCTTGAGAGCAGTGCCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgcagaaataatatactaagagcaagtttaatagtatagcccactattagctccaattcatctatagtcaatctaatagccaattcatacaat-
 ||| | ||  ||||  | || |  || ||| |  |||||||  | |||||||||||||| |||||||||||| | |||||||| || |||||  |||||||| | ||     ||   |    | || ||   ||   |  | ||    |        ||||||| |   | |||      |  |    |  ||  |    
GGGTTTAGCACTTGCTAAAGATTTGGGGTGTTTGCTTCTTGAATGTAGTGCCAAAACAAGGGAAAATGTGGAGCAGTGCTTTGAGGAACTTGCTCTAAAGgtacagtttttagaagtttgc-ttcagtgctgtttatgtcttgtacataagacaactttccaattaactgacagtagggtcatgaattcacccaagcttaa

upper sequence: LOC_Os03g05280.1 (Oryza sativa), 5'ss of exon 5
lower sequence: Vv08s0007g04740.t01 (Vitis vinifera), 5'ss of exon 5
AGGTCTTGCCTTTGCCCAGGAATCTGGATGTCTTTTTCTTGAGAGCAGTGCCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgcagaaataatatactaagagcaagtttaatagtatagcccactattagctccaattcatctatagtcaatctaatagccaattcat----acaat----
 ||| ||||  ||||  | ||   ||||||  | ||| ||||  | ||||| |||||  ||||||||||||||||||||||||||||||||||||||||||||  |   || || ||||||  |  |  |   || ||  |  | |     | |       | || |    || ||  |  |  |  |||||||     || ||    
GGGTATTGCTCTTGCAAAAGAGCATGGATGCTTATTTTTTGAATGTAGTGCTAAAACTCGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgta--cccccataagtaatat-ttgtgctc---ttcaactgccttgtaggatctctaaggtactttagtagtaatc--ttcagaattcaattcaccttgacgatggaa

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86545325|gb|CI185531.1|CI185531
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|86592468|gb|CI253149.1|CI253149
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|33691502|gb|CF319741.1|CF319741
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|89178530|gb|CI015235.1|CI015235
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|33688585|gb|CF316824.1|CF316824
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCCTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|33654292|gb|CF276906.1|CF276906
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|125863360|gb|EL586679.1|EL586679
EST:     CAAAACAAGAGAAAATGTGGAGAAATGC-TTGNAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CAAAACAAGAGAAAATGTGGAGAAATGCTTTG-AAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|88201501|gb|CI201856.1|CI201856
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|88844832|gb|CI022186.1|CI022186
EST:     TGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTNCAGTTGTCAAGAG
genomic: TGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTT-CAGTTGTCAAGAG
EST: gi|89190985|gb|CI392838.1|CI392838
EST:     GAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGA-G
genomic: GAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|33693315|gb|CF321554.1|CF321554
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
EST: gi|86845584|gb|CI346928.1|CI346928
EST:     CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAG                         ATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG
genomic: CCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 40 (upstream exon), 75 (downstream exon)
Score: 19

AGGTCTTGCCTTTGCCCAGGAATCTGGATGTCTTTTTCTTGAGAGCAGTGCCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgc ... cctatttcagATTCTTGAGGTACCAAGTCTCTTGGAGGAAGGTTCTTCAGTTGTCAAGAGGAACTCTCTGAAACAGAAGCACGAGAACAAAGCAAAAAATGGAGGATGCT




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AGGTCTTGCCTTTGCCCAGGAATCTGGATGTCTTTTTCTTGAGAGCAGTGCCAAAACAAGAGAAAATGTGGAGAAATGCTTTGAAGAGCTTGCGTTAAAGgtatacttgcagaaataatatactaagagcaagtttaatagtatagcccactattagctccaattcatctatagtcaatctaatagccaattcatacaat

- - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCTT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - agaaataa