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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TTCTGAGCTTGGTGTTTCGTTAGCCCAACTTGCTATCGCGTGGTGCGCATCGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttccacacgcccgcgtgcttcacaagatatgtttcttttgccaagtaagacgccaattaatggtttttctgaaactaagaatactaatccatcatttggtaactaaaaatgagcag

Basic information

species Oryza sativa
transcript LOC_Os02g57240.1
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os02g57240.1 (Oryza sativa), 5'ss of exon 3
lower sequence: GRMZM2G142661_T01 (Zea mays), 5'ss of exon 3
TTCTGAGCTTGGTGTTTCGTTAGCCCAACTTGCTATCGCGTGGTGCGCATCGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttccacacgcccgcgtgcttcacaagatatgtttcttttgccaagtaagacgccaattaatggtttttctgaaactaagaatactaatccatcatttggtaactaaaaatgagcag
  |||| ||||| ||||||||||| || ||  | || || |||||||| |||||||| || || || |||||||||||||||||||| ||||||| |||||||| ||        |||      ||  |||               |||||  |      | || ||    ||  |  |     |  |  |                              
CGCTGAACTTGGCGTTTCGTTAGCTCAGCTCTCGATTGCTTGGTGCGCGTCGAACCCCAATGTGTCGTCTGTGATCACTGGAGCCACCAAAGAAAGCCAGgtacatttt------ccgaacttctcctaag---------------caagtgcggtagtgaatactgaccgtttggcgaaccgcacgagcag-----------------------------

upper sequence: LOC_Os02g57240.1 (Oryza sativa), 5'ss of exon 3
lower sequence: GRMZM2G356938_T01 (Zea mays), 5'ss of exon 3
TTCTGAGCTTGGTGTTTCGTTAGCCCAACTTGCTATCGCGTGGTGCGCATCGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttccacacgcccgcgtgcttcacaagatatgtttcttttgccaagtaagacgccaattaatggtttttctgaaactaagaatactaatccatcatttggtaactaaaaatgagcag
  |||| || || ||||||||||| |||||  | || || |||||||| || ||||| ||||||||||| ||||||||||||||||| ||||||| |||||| | ||   |   | |  ||| |   |     || |    ||    ||| ||      |   |||  | |  |      |  | | || |    | ||                      
CGCTGAACTCGGCGTTTCGTTAGCTCAACTCTCCATTGCTTGGTGCGCGTCAAACCCCAACGTCTCATCCGTGATCACTGGAGCCACCAAAGAAAGCCAGgtgcattttccgaactccggtgttcttctgaggatctcgacttctgtaagcaatgtagtagggaatactctgacccg--tttggcaaaccacatgaacag---------------------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|89180694|gb|CI009602.1|CI009602
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88432104|gb|CI562935.1|CI562935
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88220082|gb|CI219642.1|CI219642
EST:     CGAACCCCAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88509171|gb|CI436677.1|CI436677
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|29661562|gb|CB657837.1|CB657837
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86834830|gb|CI345914.1|CI345914
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86865452|gb|CI330925.1|CI330925
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|87032058|gb|CI362138.1|CI362138
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCCCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86538876|gb|CI179082.1|CI179082
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88517764|gb|CI445418.1|CI445418
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88411525|gb|CI519049.1|CI519049
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         GTTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86545166|gb|CI185372.1|CI185372
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|87010046|gb|CI279143.1|CI279143
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|87030286|gb|CI270296.1|CI270296
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86874577|gb|CI318136.1|CI318136
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|58684183|gb|CK072870.1|CK072870
EST:     CGAACCCCAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88997735|gb|CI390315.1|CI390315
EST:     AACCCAAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGA
genomic: AACCC-AAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACC-AGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGA
EST: gi|88266018|gb|CI376920.1|CI376920
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88260659|gb|CI362952.1|CI362952
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|89167493|gb|CI108894.1|CI108894
EST:     CGAACCCAGACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|12623059|gb|AU173272.1|AU173272
EST:     AAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: AAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88480255|gb|CI417526.1|CI417526
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|14192249|gb|AU184460.1|AU184460
EST:     CGAACCCAAACGTNTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88438944|gb|CI531124.1|CI531124
EST:     CCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|5002919|gb|AU068028.1|AU068028
EST:     CGANCCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAANCCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|87020196|gb|CI261060.1|CI261060
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88217419|gb|CI217268.1|CI217268
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88482501|gb|CI419772.1|CI419772
EST:     CGAACCCCAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86537043|gb|CI177249.1|CI177249
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88457470|gb|CI536868.1|CI536868
EST:     ACTGGAGCCACAAAAGAAAACCAG                         GTTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: ACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86421515|gb|CI057504.1|CI057504
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|87030731|gb|CI361945.1|CI361945
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86544445|gb|CI184651.1|CI184651
EST:     CATCTGTGATCACTGGAGCCACTAAAGTAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|88224253|gb|CI224102.1|CI224102
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86536825|gb|CI177031.1|CI177031
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86540851|gb|CI181057.1|CI181057
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
EST: gi|86501617|gb|CI144250.1|CI144250
EST:     CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAG                         ATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA
genomic: CGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 64 (upstream exon), 75 (downstream exon)
Score: 30

TTCTGAGCTTGGTGTTTCGTTAGCCCAACTTGCTATCGCGTGGTGCGCATCGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttcca ... aaatgagcagATTGTTGAAAACATGAAGGCCCTCGATGTCATTCCGCTGCTAACCCCAGAAGTCGTCGACAAGATCGAAGCGGTGGTCCAAAGCAAGCCGAAGCGCACCG




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TTCTGAGCTTGGTGTTTCGTTAGCCCAACTTGCTATCGCGTGGTGCGCATCGAACCCAAACGTCTCATCTGTGATCACTGGAGCCACAAAAGAAAACCAGgtacgttccacacgcccgcgtgcttcacaagatatgtttcttttgccaagtaagacgccaattaatggtttttctgaaactaagaatactaatccatcatttggtaactaaaaatgagcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA