Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CAACATTGCCACACAGACTGTGGCAGGTTACATCTTCCTTGTTGTGGCAGCGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacggtttcttttttctcctgcgctgaagtgctgaacaatgttatcattgcacagatttactgatatttcttattttgcag

Basic information

species Oryza sativa
transcript LOC_Os01g05670.1
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os01g05670.1 (Oryza sativa), 5'ss of exon 3
lower sequence: GRMZM2G481843_T01 (Zea mays), 5'ss of exon 3
CAACATTGCCACACAGACTGTGGCAGGTTACATCTTCCTTGTTGTGGCAGCGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacggtttcttttttctcctgcgctgaagtgctg-aacaatgttatcattgc----acagatttactgatatttcttattttgcag---------
|||||| ||||||||||| |||||||||||| |||||||||| ||||| ||| | ||||||||||||||||| || || |||||| ||||||| |||||||||||  | |||  |   | |        |    || |   |  || |   |||| |    |||    ||     | | |  | ||              
CAACATCGCCACACAGACCGTGGCAGGTTACGTCTTCCTTGTCGTGGCGGCGGGCATCATGACCAACTGGGCGCTCGGCAAGCACAGCCGTCTGAAGAAGgcaagtgccgttcttccctgcgtacttttttttttgatacgaggatatccccatttcttttacatgcgtaacagaaatacagagttcattagaagcatac

upper sequence: LOC_Os01g05670.1 (Oryza sativa), 5'ss of exon 3
lower sequence: Vv13s0019g01260.t01 (Vitis vinifera), 5'ss of exon 3
CAACATTGCCACACAGACTGTGGCAGGTTACATCTTCCTTGTTGTGGCAGCGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacggtttcttttttctcctgcgctgaagtgctgaacaatgttatcat---tgcacagatttactgatatttcttattttgcag---------------
||| ||||| ||||||||||| || || ||  |||| |||||||| || ||   |||||||||||||||||| |||| ||||||||| ||| | | ||||| ||     | || | | || |||||  |  ||||  |   ||||  | |        |  | |    |   |  ||||||      |                
CAATATTGCAACACAGACTGTTGCGGGCTATGTCTTTCTTGTTGTTGCTGCTGCTATCATGACCAACTGGGCCCTTGCAAAGCACCGTCGTTTGAGGAAGgtaactt--gcttttctcttttcctgaac--aagttatttccaattcttttgcatactcttaaagaggcatgtgcttcttacacaataaataggggctctaatt
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|218483348|gb|FG969976.1|FG969976
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88838484|gb|CI375032.1|CI375032
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88448930|gb|CI558002.1|CI558002
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88264363|gb|CI368137.1|CI368137
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88409017|gb|CI556725.1|CI556725
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88217260|gb|CI217109.1|CI217109
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|58596202|gb|CK006730.1|CK006730
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGG
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGG
EST: gi|38565884|gb|CK085337.1|CK085337
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|86493307|gb|CI135940.1|CI135940
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88277995|gb|CI398797.1|CI398797
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAN                         TTGTTCGATGGCAAGGAAGGGAGGGCCGAAGTATCCTCGGCGATGGGTGAT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGA-GGCCGAAGTATCCTCGGCGATGGGTGAT
EST: gi|29686575|gb|CB682850.1|CB682850
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88465656|gb|CI522407.1|CI522407
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAN                         TTGTTCGATGGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGAT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGAT-GGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGAT
EST: gi|86535695|gb|CI175901.1|CI175901
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|86868360|gb|CI321670.1|CI321670
EST:     TCATGACCAACTGGGCACTTGG-AAGCACCCCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGAGAGGCCGAAGTATCCTCGGCGATGGGGTGAT
genomic: TCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGAT-GGGTGAT
EST: gi|29688041|gb|CB684316.1|CB684316
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88289581|gb|CI522714.1|CI522714
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|27578144|gb|CB000839.1|CB000839
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|12405077|gb|AU166678.1|AU166678
EST:     GAAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: G-AGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|86256425|gb|CI061188.1|CI061188
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88830451|gb|CI010651.1|CI010651
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|86251148|gb|CI055281.1|CI055281
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAAGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88375921|gb|CI528225.1|CI528225
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCCCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|66924234|gb|CX111082.1|CX111082
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|87011468|gb|CI279377.1|CI279377
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88966120|gb|CI012996.1|CI012996
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88253275|gb|CI351901.1|CI351901
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88256534|gb|CI358922.1|CI358922
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|88474410|gb|CI411681.1|CI411681
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCCCCGTTTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|58666456|gb|CK055142.1|CK055142
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|33653574|gb|CF276188.1|CF276188
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|33655192|gb|CF277806.1|CF277806
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|27576649|gb|CA999343.1|CA999343
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|29684478|gb|CB680753.1|CB680753
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|29688042|gb|CB684317.1|CB684317
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
EST: gi|86536046|gb|CI176252.1|CI176252
EST:     CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAG                         TTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT
genomic: CGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 40 (upstream exon), 73 (downstream exon)
Score: 28

CAACATTGCCACACAGACTGTGGCAGGTTACATCTTCCTTGTTGTGGCAGCGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacgg ... tattttgcagTTGTTCGATGGCAAGGAAGGGAGGCCGAAGTATCCTCGGCGATGGGTGATTCTTCCACCATTCCTGTAAGGAGTTGGTGTCAGCACGATGGCCACATGGG




<











<


<









Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CAACATTGCCACACAGACTGTGGCAGGTTACATCTTCCTTGTTGTGGCAGCGAGTATCATGACCAACTGGGCACTTGGAAAGCACCGCCGTCTAAAGAAGgcaagcacggtttcttttttctcctgcgctgaagtgctgaacaatgttatcattgcacagatttactgatatttcttattttgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG