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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GGCGCAGCAGCACTAGCCGCGGTATAAAACATCCAAGCGCTCCTCCATCGTCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactttgtcctccaccttgatcgctgttgctaatctccgggaaaaaaggaaatcgtctttttcttcttcttttttcttcttgttttgcttcggcg...

Basic information

species Oryza sativa
transcript LOC_Os04g40874.1
intron # 1
splice site 5'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86890781|gb|CI303972.1|CI303972
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTTCAGGAGG
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATG-TCAGGAGG
EST: gi|88421242|gb|CI569087.1|CI569087
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88383894|gb|CI631784.1|CI631784
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCANCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|29663317|gb|CB659592.1|CB659592
EST:     CCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: CCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88338649|gb|CI644106.1|CI644106
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88348462|gb|CI629951.1|CI629951
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGNACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88373258|gb|CI614594.1|CI614594
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88411783|gb|CI571275.1|CI571275
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88313812|gb|CI597443.1|CI597443
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88449978|gb|CI572109.1|CI572109
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGGAGG
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCA-GGAGG
EST: gi|58654814|gb|CK043494.1|CK043494
EST:     CCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCGAGGGTATTTATCATGTCAGGAGG
genomic: CCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACC-AACCTATTTATCATGTCAGGAGG
EST: gi|88719764|gb|CI743274.1|CI743274
EST:     GCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: GCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88421660|gb|CI616985.1|CI616985
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGGCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88447483|gb|CI584598.1|CI584598
EST:     TCGCCACCGCT-CGGGTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|58611861|gb|CK039573.1|CK039573
EST:     CTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: CTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88388045|gb|CI590321.1|CI590321
EST:     CGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: CGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88765888|gb|CI774978.1|CI774978
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88305522|gb|CI630684.1|CI630684
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88407965|gb|CI588994.1|CI588994
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTGAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88430411|gb|CI567430.1|CI567430
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATATATCATGTCAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88403389|gb|CI573112.1|CI573112
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88409948|gb|CI571205.1|CI571205
EST:     TCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTTTATTGCTGAAGGAAGAAGAGCACCAACTTATTTATCATGTCAGGAGGA
genomic: TCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88737684|gb|CI761192.1|CI761192
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|29634314|gb|CB639323.1|CB639323
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|29614696|gb|CB619709.1|CB619709
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88396043|gb|CI595591.1|CI595591
EST:     CCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: CCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88421686|gb|CI569067.1|CI569067
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88727823|gb|CI749335.1|CI749335
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88363986|gb|CI607296.1|CI607296
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88300652|gb|CI587456.1|CI587456
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|58670061|gb|CK058747.1|CK058747
EST:     CTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGG
genomic: CTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGC-TGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGG
EST: gi|29680803|gb|CB677078.1|CB677078
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|86821857|gb|CI309286.1|CI309286
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88417228|gb|CI576302.1|CI576302
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|29635386|gb|CB640395.1|CB640395
EST:     CGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: CGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|325493796|gb|JG458320.1|JG458320
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAG
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAG
EST: gi|88352601|gb|CI624540.1|CI624540
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88363865|gb|CI607175.1|CI607175
EST:     ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: ACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|86904132|gb|CI304566.1|CI304566
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|117243651|gb|CT843162.1|CT843162
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
EST: gi|88733223|gb|CI756400.1|CI756400
EST:     TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCG                         GTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA
genomic: TCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 75 (upstream exon), 73 (downstream exon)
Score: 37

GGCGCAGCAGCACTAGCCGCGGTATAAAACATCCAAGCGCTCCTCCATCGTCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactt ... cttttcacagGTATATTGCTGAAGGAAGAAGAGCACCAACCTATTTATCATGTCAGGAGGATCTTCACCAAGGTCAAGACGAAGCAGCTTTAATTCTTTATCAAGAGATC




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GGCGCAGCAGCACTAGCCGCGGTATAAAACATCCAAGCGCTCCTCCATCGTCGCCACCGCTCCGGTTTGCCTCCCGCGCGAGCCCTCGTCGACGCCGCCGgtcagtactttgtcctccaccttgatcgctgttgctaatctccgggaaaaaaggaaatcgtctttttcttcttcttttttcttcttgttttgcttcggcg

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctccacc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gaaaaaa