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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag

Basic information

species Glycine max
transcript GLYMA08G18760.3
intron # 6
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: LOC_Os06g02380.2 (Oryza sativa), 5'ss of exon 7
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttg---tttccagatgt-tgataagatatgtattttctcaattggtttacag--------
||||| |||||||| ||| |||| ||||| ||  | || |||||||| ||||| |||||||||| ||||||| | ||||||||||||||  | |||||||||         |    || |  ||| |||| ||  |  |   |  ||     || |||   ||  || ||     ||  | ||  |||| |    ||  | ||   |||   |||            
AACAAGCTTAGAGGGTCATTGAAAATTGCCGCTATCAAAGCCCCTGGTTTTGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtggacttgcgatctggtgcatttggttgggtgatcattaatgctgcttatttatgatgattctggagcatctggctggatgattaatgctgcttatttataatgattttttttctctgcag

upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: LOC_Os02g01280.1 (Oryza sativa), 5'ss of exon 7
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgt---ctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag
||||  ||||| ||  || |||||||||| ||  | || || ||||| |||||||| |||||||| || ||||||||||||||||| |   | |||||||||    || |  | |  | ||    || ||    | |   |     |  |  |||| | |    ||| || |  || |||                                      
AACAGGCTTAGGGGTGCATTGAAGATTGCTGCTATTAAAGCTCCTGGTTTTGGAGAACGCAAGAGTCAATACCTTGATGATATTGCTACTCTTACTGGAGgtgatattcagcttttgacataagttgtctacactct--aagccacacgtgcgcttatat----atcgttcttctttcag--------------------------------------

upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: GRMZM2G095252_T02 (Zea mays), 5'ss of exon 1
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag
||||| || ||||||||| |||||||||| ||  | || |||||||| ||||||||||||||||  |||||| | ||||||||||||||| | ||||||||||  | ||  |     | |   || |    || ||    |||||      | ||| |      | ||||||||   |                                     
AACAAGCTCAGAGGATCATTGAAGATTGCTGCCATCAAAGCCCCTGGTTTTGGAGAGCGCAAGACTCAGTACTTGGATGATATTGCCATCCTTACTGGAGgtcagttaaacttccgttctagagcatcc--ttatgatcatgtatgctccttgtttataagcattctttgtttctgcag------------------------------------

upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: GRMZM2G083716_T04 (Zea mays), 5'ss of exon 6
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag
||||| || ||||||||||||||||| || ||  | || |||||||| ||||||||||||||||  |||||| | ||||||||||||||| | ||||||||||    ||  |   | | | | || |   ||||||    |||||  |   | |   |        ||||||||   |                                     
AACAAGCTCAGAGGATCACTGAAGATCGCTGCCATCAAAGCCCCTGGTTTTGGAGAGCGCAAGACTCAGTACTTGGATGATATTGCCATCCTTACTGGAGgtcagctaaacttccattctaaagcttc--attttgatcatgtatgcaccttatccataaacatactttgtttctgcag------------------------------------

upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: GRMZM2G015989_T01 (Zea mays), 5'ss of exon 7
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag
|| |  ||||||||  || |||||||||| ||  | ||||||||||| |||||||||||||| || || || |||||||| ||||| |   | |||||||||             || || ||  ||||  || ||     |||||  |   ||||||  | ||   |  |      || ||||   |  ||   ||  | ||            
AATAGGCTTAGAGGTGCATTGAAGATTGCTGCTATTAAGGCCCCTGGTTTTGGAGAGCGCAAAAGTCAATATCTTGATGACATTGCTACTCTCACTGGAGgt------------gaactttat--ctttagttatgatttggtatacct-tattttttggtgtcagatgatc-----attttgaccggccatac-ttcacgcag-----------

upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: AT1G55490.2 (Arabidopsis thaliana), 5'ss of exon 7
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtct-ttaaatataaaaatttcatgcctttgattttggaagtgtatatatgctttttttt---ctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag
||||| ||||||||  |||||||||| ||||| || |  || || ||||||||||||||||||||||| ||||||||||||||||||||  | |||||||||   ||   | |  |    | |  ||| || ||  | |  | | | ||   ||| | ||   |||  ||| | | | |   ||  |                                
AACAAGCTTAGAGGTACACTGAAGATAGCAGCTCTCAGAGCTCCAGGGTTTGGAGAGCGCAAGAGCCAATACCTTGATGATATTGCCATTCTAACTGGAGgtaagttctgtctgcattgatgagccctatgtttacgcatatatttgtagtttttatcttgtcctaattttctatcttcgcttgcag--------------------------------

upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: AT3G13470.1 (Arabidopsis thaliana), 5'ss of exon 7
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgt--------ctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag
||||| ||||||||  |||||||||||||||| || || || ||||| |||||||||||||||||||| |||||||| |||||||||||  | |||||||||         ||| |  | |||||    ||   | ||  | |  |    | |     | |  |||   ||| ||| | |   | |   | |       |||   ||||   || || | |||
AACAAGCTTAGAGGCACACTGAAGATTGCAGCTCTCAAAGCTCCTGGATTTGGAGAGCGCAAGAGCCAATACCTTGACGATATTGCCATTCTAACTGGAGgtaggtcatgtttttataacaaaaaacctata--tgtgggtatcaatcaatgtgccctcctgatttggaatcatttggctgttaaaaccttac----cgtgttgcttctgtttttgtgtgcag

upper sequence: GLYMA08G18760.2 (Glycine max), 5'ss of exon 6
lower sequence: Vv06s0004g00240.t01 (Vitis vinifera), 5'ss of exon 7
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag
|| || |||||||| ||| | |||||||| ||||| || |||||||| |||||||||||||||||||||||||| ||||| ||||| ||  |||||||||||    | | |||| | |||||    |||  ||||     | || || |   ||||  |  |   |||| ||   | ||  ||  |  || | ||||| | ||            
AATAAGCTTAGAGGGTCATTAAAGATTGCTGCACTTAAAGCCCCTGGATTTGGAGAGCGCAAGAGCCAGTACCTGGATGACATTGCAATTCTGACTGGAGgttagcatagataacattttcac-tatttttttttcttcttctaaat-tttattttacttgagagttttattctaatattctgtcattat-tatatttgcaca------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|298191386|gb|HO038865.1|HO038865
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|17964146|gb|BM270885.1|BM270885
EST:     GAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: GAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16281997|gb|BI944781.1|BI944781
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|15589931|gb|BI674547.1|BI674547
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16284918|gb|BI946338.1|BI946338
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16282846|gb|BI945236.1|BI945236
EST:     CAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: CAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|151401848|gb|EV271661.1|EV271661
EST:     ACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: ACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|20075657|gb|BQ094440.1|BQ094440
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|26268602|gb|CA819665.1|CA819665
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|193353569|gb|FK319365.1|FK319365
EST:     TGGAGATCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTTGGCCTTACTTTGGACAAAGCTGGGAAA
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGG-TTGGCCTTACTTTGGACAAAGCTGGGAAA
EST: gi|208238870|gb|GE036346.1|GE036346
EST:     TGGAGATCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|15813847|gb|BI786122.1|BI786122
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16276796|gb|BI942048.1|BI942048
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|207792803|gb|GD764583.1|GD764583
EST:     TGGAGATCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16279198|gb|BI943310.1|BI943310
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|120530550|gb|EH258683.1|EH258683
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16278942|gb|BI943174.1|BI943174
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|27426513|gb|CA938033.1|CA938033
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|298172173|gb|HO021765.1|HO021765
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|298196897|gb|HO041640.1|HO041640
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16284535|gb|BI946141.1|BI946141
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|151412595|gb|EV282407.1|EV282407
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|16278862|gb|BI943133.1|BI943133
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
EST: gi|207735456|gb|GD710299.1|GD710299
EST:     TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAG                         GTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG
genomic: TGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaat ... tggtttacagGTACTGTAATCAGAGAAGAGGTTGGCCTTACTTTGGACAAAGCTGGGAAAG

Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AACAAACTTAGAGGATCACTGAAGATTGCAGCACTGAAGGCCCCTGGGTTTGGAGAGCGCAAGAGCCAGTACCTTGATGATATTGCCATCTTGACTGGAGgtctttaaatataaaaatttcatgcctttgattttggaagtgtatatatgcttttttttctatctttttgtttccagatgttgataagatatgtattttctcaattggtttacag

- - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAGA