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 5'  3'   		2  
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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AAAATTGGCTAGGTTGAAGCAAGCCAAGGAAGAGGCTGAAAAGGAGATTGCTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctatgattatattttgatgctgttaagaccatccttttacattattctaatttatgatgtattctcctactccaacttgtgtgtgtgctttcttattattactgcag

Basic information

species Glycine max
transcript GLYMA10G06010.2
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GLYMA10G06010.2 (Glycine max), 5'ss of exon 3
lower sequence: Vv08s0040g01910.t01 (Vitis vinifera), 5'ss of exon 2
AAAATTGGCTAGGTTGAAGCAAGCCAAGGAAGAGGCTGAAAAGGAGATTGCTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctatgattatattttgatgctgttaagaccatccttttacattattctaatttatgatgtattctcctactccaacttgtgtgtgtgctttcttattattactgcag
|||| |||||||  | || |||||||| ||||||||||||||||||||||||   |||||||| ||  | ||   |||||| || |||||| || | || ||||||  ||   |  |||  |    |    | |  |     | | |||   |||  ||| |   | |   ||| || || |       | ||   | | |  || | |    
AAAAATGGCTAGACTAAAACAAGCCAAAGAAGAGGCTGAAAAGGAGATTGCTGCTTACCGAGCCCAGGTTGAACTTGAGTTCCAGAAGAAATTGGCTGAGgtaagt--ataggtggattcagtccattcataaagaactgagtcagattgaccta--ttaaggcttctgagccttcttcaccc-----atatggcatgtcacaatcaat----
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|209697418|gb|BW675563.1|BW675563
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|209706506|gb|BW676080.1|BW676080
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|193635806|gb|FK580371.1|FK580371
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAAGAAGAACT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAA-GA-GA-CT
EST: gi|192294406|gb|FG987335.1|FG987335
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|14206383|gb|BG840061.1|BG840061
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGNC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|7042134|gb|AW472028.1|AW472028
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|151395376|gb|EV265247.1|EV265247
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|209706507|gb|BW676081.1|BW676081
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|192308452|gb|FK002824.1|FK002824
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|208283924|gb|GE082428.1|GE082428
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|15285912|gb|BI469803.1|BI469803
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|192327388|gb|FK018150.1|FK018150
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|193553486|gb|FK510025.1|FK510025
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|192325567|gb|FK019423.1|FK019423
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|31465211|gb|CD407239.1|CD407239
EST:     AAAGAAAGTGTCAGCC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: AAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|208266902|gb|GE060967.1|GE060967
EST:     CTCAATACCGAGCTCAACTAGAGGCTGGGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGG
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGG
EST: gi|8283562|gb|BE021121.1|BE021121
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|193719310|gb|FK653476.1|FK653476
EST:     TCAATACCGAGCTCTAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: TCAATACCGAGCTC-AACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|58024531|gb|CX711272.1|CX711272
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|209697417|gb|BW675562.1|BW675562
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|192311487|gb|FK005218.1|FK005218
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|120531416|gb|EH259549.1|EH259549
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|192328300|gb|FK020011.1|FK020011
EST:     CTCAGTACCGAGCTCAACTAGAGGCCGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|192314767|gb|FK006665.1|FK006665
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|21601632|gb|BQ611963.1|BQ611963
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|12688761|gb|BG155097.1|BG155097
EST:     ATANCCGAGCTCAANCTAGAGGGCTGAGTTTCAAAANGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: ATA-CCGAGCTCAA-CTAGA-GGCTGAGTTTCAAAA-GAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|12688293|gb|BG154629.1|BG154629
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTTGA
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACT-GA
EST: gi|208222512|gb|GE016995.1|GE016995
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|19938094|gb|BQ081557.1|BQ081557
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|192325874|gb|FK016551.1|FK016551
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|208129637|gb|GD928937.1|GD928937
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|22540338|gb|BU090181.1|BU090181
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|31460442|gb|CD402410.1|CD402410
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|120492606|gb|EH220773.1|EH220773
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|151401027|gb|EV270841.1|EV270841
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|9898861|gb|BE607829.1|BE607829
EST:     AAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: AAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|208312511|gb|GE113807.1|GE113807
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
EST: gi|193318591|gb|FK285049.1|FK285049
EST:     CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGAC                         AGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT
genomic: CTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctat ... attactgcagAGTACTGGAGATTCTGGAGCTAATGTCAAGCGGCTTGAGCAAGAGACTGAT

Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AAAATTGGCTAGGTTGAAGCAAGCCAAGGAAGAGGCTGAAAAGGAGATTGCTCAATACCGAGCTCAACTAGAGGCTGAGTTTCAAAAGAAAGTGTCAGACgtaagtctatgattatattttgatgctgttaagaccatccttttacattattctaatttatgatgtattctcctactccaacttgtgtgtgtgctttcttattattactgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA