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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaattccgtttttcatattttaggaaaatccatcactggagcgagttgattctggaaccagtattcatttgtgcttattactttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT

Basic information

species Zea mays
transcript GRMZM2G172427_T01
intron # 9
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G172427_T01 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os02g47900.1 (Oryza sativa), 3'ss of exon 5
-----------------------------------gtaattccgttttt--catattttagga--aaatccatcactggagcgagttgattctggaaccag--tattc----atttgtgcttat-tactttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT
                                    ||| |  |   ||   ||||||| |||   ||    |   |||    |  | | |  || |  |   | |||    |    ||||| |   | || ||  ||| |||||||| ||||||||||| || || |||| ||| ||||||||||| || |||||||| |||||||| |||||||| || || |||
gtaactggactattttatgtctgtcccaaaatatattaacttagaagttaatatattttgggatggaaggagtacttgggaacatcttactacgggataaatttgttctggaaccaatgcttttgccccttcattgcagCTGTTCCCCAAATCTCAGTACGCGTTTAGTTTCGGTGGAGAGCAAAGACTGTCAGCTTGCGCACATTGGTTTGTTTGCAAACCAGGAT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67021860|gb|CO450609.1|CO450609
EST:     TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAG                         TTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG
genomic: TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG
EST: gi|10191692|gb|BE761768.1|BE761768
EST:     TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAG                         TTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG
genomic: TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG
EST: gi|32832594|gb|CD972272.1|CD972272
EST:     TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAG                         TTGTTCCCCGAATCTCAGTA
genomic: TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTA
EST: gi|14701180|gb|BI233598.1|BI233598
EST:     T-ANTTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAG                         TTGTTCCCCGAATCTCAGTACCCG
genomic: TTA-TTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCG
EST: gi|69351242|gb|DR452068.1|DR452068
EST:     TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAG                         TTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG
genomic: TTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG
EST: gi|83278797|gb|DV942805.1|DV942805
EST:     GAAATGTTTCCCGTTATATTAGTCACAG                         TTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG
genomic: GAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 197

CTTCCCAGATTGATAGGGAAAGAGTTCGGACTGTAGGGGCTATTGAATACTTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT


Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 66

CTTCCCAGATTGATAGGGAAAGAGTTCGGACTGTAGGGGCTATTGAATACTTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 110

CTTCCCAGATTGATAGGGAAAGAGTTCGGACTGTAGGGGCTATTGAATACTTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 156

CTTCCCAGATTGATAGGGAAAGAGTTCGGACTGTAGGGGCTATTGAATACTTTATTGATGCCACAAGGTCTGGAAATGTTTCCCGTTATATTAGTCACAGgtaattccgt ... ttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ggagcgagttgattctggaaccagtattcatttgtgcttattactttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT
                                           ctttgat  putative branch site (score: 156)
                                        ttacttt  CT-rich tract
                                     ttattactttgataa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaattccgtttttcatattttaggaaaatccatcactggagcgagttgattctggaaccagtattcatttgtgcttattactttgataacagTTGTTCCCCGAATCTCAGTACCCGGTTGGTTTTGGTAGAGAGCAAAGATTGCCAGCTTGCTCACATTGGATTGTTTGCTAATCAAGAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAAG