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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaaaaaagtctgcatcatgtgctgcggtattatctgtcataatgtggtagtgtgtttgatacatttgtctttctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG

Basic information

species Zea mays
transcript GRMZM2G115245_T01
intron # 9
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G115245_T01 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os11g08330.1 (Oryza sativa), 3'ss of exon 25
gtaaaaaagtctgcatcatgtgctgcggtattatctgtc-ataatgtggtagtgt--gtttgatacatttgtctttctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG
|||||   |  || |   |    ||| |  | || | |  ||  |||| | |||   |||||||  |    || |||| | || ||||||||||||  ||||||||||| |||||||||||||| ||||| ||||| || ||||| ||||||||||||||||||||||| ||||| 
gtaaacttgaatgtactttcaattgcagagtcatgtatttatcttgtgatggtgatagtttgatgaa---atccttcttcttgtagGCATATGAGAGGTCAGAAGATCCTATTTATGTTTTGGATAATAACATACCAATAGATCCCCAATACTACCTTGAGAACCAAATCAGCAAA

upper sequence: GRMZM2G115245_T01 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA06G16110.1 (Glycine max), 3'ss of exon 25
gtaaaaaa-gtctgcatcatgtgctgcggtattatctgtcataatgtggtagtgtgtttgatacatttgtctttctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG
|||| | | |||  | || | |  ||  ||    | | | |  || || |    | ||   || | |    | |  | |  ||||||||||||| |||||| ||||| || |||||  | || || || |||||||| |||||||| ||||| |||||||| ||||||||||||
gtaatacatgtccccctctttttttgaagt----tttttaaacatttgtttagattttctctagactaacgtgtaagaaatcagGCATATGAGAGATCAGAGGATCCTATCTATGTGCTAGAGAACAACATACCCATAGATCCTCATTACTATCTTGAGAATCAAATTAGCAAG

upper sequence: GRMZM2G115245_T01 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA04G38800.2 (Glycine max), 3'ss of exon 9
gtaaaaaagtctgcatcatgtgctgcggtattatctgtcataatgtggtagtgtgtttgatacatttgtctttctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG
|||| | |     |    | |  |     | | | | | |  || || |    | ||   || |||  | | |    |  |||||||| ||||||||||| ||||| || |||||| | || || || ||||| || |||||||| ||||| |||||||| ||||||||||||
gtaatacataaccccctcttttttttaaaaatgtttttaaacatctgtttagattttctgtagattaatgtgtaataaatcagGCATACGAGAAATCAGAGGATCCTATCTATGTTCTAGAGAACAACATACCAATAGATCCTCAGTACTATCTTGAGAATCAAATTAGCAAG

upper sequence: GRMZM2G115245_T01 (Zea mays), 3'ss of exon 9
lower sequence: PP1S54_271V6.1 (Physcomitrella patens), 3'ss of exon 25
---------------------------------------------------------------------------gtaaaaaagtctgcatcatgtgctgcggtattatctgtcataatgtggtagtgtgtttgatacatttgtctttctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG
                                                                            |||       ||    | | |    |    ||  |      |  |  ||    |      | ||   | | ||  | || |||| |||||||| ||||||||||| || ||||||||||| |||||||| || |||||||| ||||||||| | || |||||  | || |||
gtatcacatgattcgtctggattattgtggagttatttgaagatgctggtgtcctactgactacgtccaaatatgctaattgctgttgtcataagagaaatgaagataagttctgcgagattctaactagagactaaaatcaatgtgtcgtcttgtagGCGTATGAGAAGTCAGAAGATCCAATCTATGTTTTGGAGAATAATATTCCGATTGATCCACAATACTACTTGGAAAACCAGCTGAGTAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32930209|gb|CF035021.1|CF035021
EST:     TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAG                         GCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
genomic: TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
EST: gi|32932138|gb|CF036950.1|CF036950
EST:     TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAG                         GCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
genomic: TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
EST: gi|32931064|gb|CF035876.1|CF035876
EST:     TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAG                         GCATATGAGAAATCAGTAGATCCGATTTATGTTTTGGACAATAATATACCC
genomic: TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
EST: gi|32931909|gb|CF036721.1|CF036721
EST:     TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAG                         GCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
genomic: TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
EST: gi|32932752|gb|CF037564.1|CF037564
EST:     TTGGTGACCGCGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAG                         GCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC
genomic: TTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 160

AGAGATCCAGCTACTGCACCTACTGTTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG


Block sizes: 47 (upstream exon), 46 (downstream exon)
Score: 17

AGAGATCCAGCTACTGCACCTACTGTTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 19

AGAGATCCAGCTACTGCACCTACTGTTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG


Block sizes: 49 (upstream exon), 37 (downstream exon)
Score: 15

AGAGATCCAGCTACTGCACCTACTGTTGGTGACCGTGTTCCTTATGTAATAATTAAAGCAGCAAAAGGGGCAAAGgtaaaaaagt ... ctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tattatctgtcataatgtggtagtgtgtttgatacatttgtctttctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG
                          gtttgat  putative branch site (score: 15)
                                    tttgtctttct  putative PPT
                               atacattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaaaaaagtctgcatcatgtgctgcggtattatctgtcataatgtggtagtgtgtttgatacatttgtctttctgcatgcagGCATATGAGAAATCAGAAGATCCGATTTATGTTTTGGACAATAATATACCCATTGATCCTCAATACTACCTTGAGAACCAAATTAGCAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCAAG