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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttcatggatgtacactccatttcagttttaaatatactttctgttaattctgctccttctaaatctctactaaacctatgctctgcattgccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG

Basic information

species Zea mays
transcript GRMZM2G093065_T01
intron # 9
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G093065_T01 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os06g43560.1 (Oryza sativa), 3'ss of exon 9
ttcatgga-tgtacactccatttcagttttaaatatactttctgttaattctgct--ccttctaaatctctactaaacctatgctctgcattgccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG
 ||||| | |   |||| | ||       | ||      | |||||| ||    |  | |||  ||||| |  |  |        ||   |    ||||| |||||||||||||  |||||||||||||||| ||||||||||||| ||| ||  |||||||| ||| ||||||| | || |||||||||||||||||| | |
-tcatgaagttctcacttccttgtgcccatgaaagcggat-ctgttagttacatttacattcacaatctgtgttctataatgatcctctctcaatctctc-agGCTCAACTTCTGTCACGAGATGATGAACAGGGCTCTTCTGATGATGGTGATAATTCTGATGGTAAATCCTCAGTTACCATGAGCAGTGAAGATGCATATG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|78092720|gb|DV521094.1|DV521094
EST:     TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAG                         GCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
genomic: TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
EST: gi|60395437|gb|DN228307.1|DN228307
EST:     TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAG                         GCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
genomic: TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
EST: gi|110207810|gb|EC889707.1|EC889707
EST:     TAGATCTTCTAGCAACATCAGACAATAGAATACGCTTACTCGTTGCTGAG                         GCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTTTGATGGTGGGGAC
genomic: TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
EST: gi|211241304|gb|FL421834.1|FL421834
EST:     TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAG                         GCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
genomic: TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
EST: gi|71774030|gb|DR971916.1|DR971916
EST:     TAGATTTTTTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAG                         GCTCAACTTTTAGCACGAGATGATGAACAAGGCTCTTTTGATGGTGGCGAC
genomic: TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
EST: gi|71316281|gb|DR794821.1|DR794821
EST:     TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAG                         GCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC
genomic: TAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 58

AGAAGAACAAGACAACTTTACTATTGATCCATCATCCTTGCCTGATGCATTAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG


Block sizes: 45 (upstream exon), 42 (downstream exon)
Score: 25

AGAAGAACAAGACAACTTTACTATTGATCCATCATCCTTGCCTGATGCATTAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 56

AGAAGAACAAGACAACTTTACTATTGATCCATCATCCTTGCCTGATGCATTAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 26

AGAAGAACAAGACAACTTTACTATTGATCCATCATCCTTGCCTGATGCATTAGATCTTCTAGCAACATCAGACAATAGAATACGCCTACTCGTTGCTGAGgtaaacttga ... ccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aattctgctccttctaaatctctactaaacctatgctctgcattgccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG
                      tactaaa  putative branch site (score: 26)
                                          ttgccctctcc  putative PPT
           ttctaaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttcatggatgtacactccatttcagttttaaatatactttctgttaattctgctccttctaaatctctactaaacctatgctctgcattgccctctccagGCTCAACTTCTAGCACGAGATGATGAACAAGGCTCTTCTGATGGTGGCGACGATTCTGATAGTAGATCCTCATTAACTATGAGCAGTGAAGATGCAAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG
- -atggatg
- - - - - - cactcca
- - - - - - - - - - - - - - - - - - - ttctgtt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ctatgct
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctgcatt