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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaactattctgttgagctttttgcaacggggcaatttttatgctatggcaccttgtgactgttatattgagtttgttctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG

Basic information

species Zea mays
transcript GRMZM2G070422_T02
intron # 9
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G070422_T02 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os08g23810.2 (Oryza sativa), 3'ss of exon 9
gtaactattctgtt-gagctttttgcaacggggcaatttttatgctatggcaccttgtgactgttatattgagtttgttctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG
|||||||   | || ||| |||     ||   |||  |||| |   ||   || |   |   || ||  ||| | | |    |  ||| ||||||||||| |||||||| ||||||||||||||||||||||| |||   
gtaactacattatttgagatttaaaatacaaagca--ttttgtatcat--tacttaccggtggtaattctgattatatatatttttagATATGGTGGTGGCATGAGTTCAGCTAAAGCAGCTCTTGAGAGTGACACAAAA

upper sequence: GRMZM2G070422_T02 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA11G10770.1 (Glycine max), 3'ss of exon 9
gtaactattctgttgagctttttgcaacggggca-atttttatgctatggcaccttgtgactgttatattga--gtttg--ttctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG
     |||| || | |     | | ||      | | || |||| |     |  ||         ||||| |   | |   |  |   | || ||||||||||| ||||||||||| ||||| || || |||||||||||||| 
----gtattttggttatgccatagaaagtccttataattctatgtttcataaatttagactgacaatattcatgatctaaataatgatacagATATGGTGGTGGTATGAGTTCTGCCAAAGCTGCACTGGAGAGTGATACACGA

upper sequence: GRMZM2G070422_T02 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA12G03060.1 (Glycine max), 3'ss of exon 10
-----gtaactattctgttgagctttttgcaacggggcaatttttatgctatggcaccttgtgactgttatattgagtttgttctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG
      |   ||| |     |  |  ||  ||        ||  ||   |  | |      |     | || |  |   || |     | || ||||||||||| ||||||||||| ||||| || || |||||||||||||| 
gtatgttggttatgcatagaaagtccttataattctatgtttcataaatttagactgacaatattcatgatctaaataatgat-----acagATATGGTGGTGGTATGAGTTCTGCAAAAGCTGCACTGGAGAGTGATACACGA

upper sequence: GRMZM2G070422_T02 (Zea mays), 3'ss of exon 9
lower sequence: AT2G05990.2 (Arabidopsis thaliana), 3'ss of exon 8
------gtaactattctgttgagcttttt---gcaacggggcaatttttatgct--atggcaccttgtgactgttatattgagtttgttctctc---atagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG
       |  || |  |    || | ||    | |||     |||    |  |   | |    |     | | | | |   |  |  |||  ||     ||||||||||| || ||||||||||| ||||| || || |||||||| ||||||
gtaaggatggct-tcttcacaagttattcaaaggaacaaactaatgcacacacataacgatgtcaacacattctcacaagtatctctttcattctgtgcagGTATGGTGGAGGTATGAGTTCTGCCAAAGCTGCACTAGAGAGTGACACACGG

upper sequence: GRMZM2G070422_T02 (Zea mays), 3'ss of exon 9
lower sequence: PP1S99_59V6.3 (Physcomitrella patens), 3'ss of exon 8
---------gtaactattctgttgagcttt---ttgcaacggggcaatttttatgctatggcaccttgtgac-tgttatattgagtttgttctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG
          |||  | |  |     | ||   || |   |    |||  |    ||  |  |  ||       |||||    | ||    |  | | || |||||||| |||||||| ||||| ||||| || ||||||||||| ||||| 
ctatgcatattaatcacttagaaagtccttaacttcctttgtttaaatcatctctctgagctagtttagagtacgttatgaccatttgtgcccttgacagATATGGTGGAGGGATGAGCTCTGCCAAAGCTGCACTTGAGAGTGACACACGT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67015153|gb|CO443902.1|CO443902
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|211267881|gb|FL003946.1|FL003946
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|31364152|gb|CD448507.1|CD448507
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|67015181|gb|CO443930.1|CO443930
EST:     CTCTCTAACATACATTGCATCTGAAA-GGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: CTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|31357208|gb|CD441565.1|CD441565
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|24768563|gb|CA403692.1|CA403692
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|32907864|gb|CF012677.1|CF012677
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|31362224|gb|CD446581.1|CD446581
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|67042246|gb|CO468501.1|CO468501
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|84980240|gb|DW530589.1|DW530589
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|9952049|gb|BE638632.1|BE638632
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|6526869|gb|AW216132.1|AW216132
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAA
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAA
EST: gi|9254230|gb|BE344698.1|BE344698
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|166615957|gb|EG102324.1|EG102324
EST:     AACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: AACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|31355683|gb|CD440040.1|CD440040
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|31364652|gb|CD449007.1|CD449007
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|32908049|gb|CF012862.1|CF012862
EST:     GAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|211497962|gb|FL450082.1|FL450082
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|211375763|gb|FL457930.1|FL457930
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|71766308|gb|DR964245.1|DR964245
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|60399474|gb|DN232286.1|DN232286
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|67022814|gb|CO451563.1|CO451563
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|13150169|gb|BG320491.1|BG320491
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|211497961|gb|FL450081.1|FL450081
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|31353471|gb|CD437828.1|CD437828
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|6526806|gb|AW216075.1|AW216075
EST:     ACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACCCG
genomic: ACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|111990916|gb|EE332354.1|EE332354
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|29544932|gb|CB605540.1|CB605540
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|32908050|gb|CF012863.1|CF012863
EST:     GGTGCTAGCATCTCTCTAACATACATTGCGTCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|32907865|gb|CF012678.1|CF012678
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
EST: gi|6564253|gb|AW231875.1|AW231875
EST:     GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGG                         GTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG
genomic: GGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 619

GTGGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 378

GTGGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 139

GTGGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1638

GTGGTGCTAGCATCTCTCTAACATACATTGCATCTGAAAGGGCGATTCCTGGgtaactattc ... tctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

caatttttatgctatggcaccttgtgactgttatattgagtttgttctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG
                                        tttgttctctc  CT-rich tract
                            tgttatatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaactattctgttgagctttttgcaacggggcaatttttatgctatggcaccttgtgactgttatattgagtttgttctctcatagGTATGGTGGTGGGATGAGTTCTGCTAAAGCAGCTCTTGAGAGTGATACACGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTC