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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaacctggaactactcatgcatgggttctcttctgtgtcacagggtcgcttcgcttcactctctctggtctggtttgctgctaactaggattttgtttatttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC

Basic information

species Zea mays
transcript GRMZM2G044247_T01
intron # 9
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G044247_T01 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os03g02690.3 (Oryza sativa), 3'ss of exon 9
gtaacctggaactactcatgcatgggttctcttctgtgtcacagggtcgcttcgcttcactctctctggtctggtttgctgctaactaggattttgtttatttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAAC-AACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC-
             |||   ||      | |||  ||||       |  | |    | || | || |||               ||  |  ||| | | |   ||||||||||||||||||||| || |||||||||||||||      |  | |   |   |   ||   ||  |   ||     ||||  | |||  | ||   |     
----------gtaactggcgc-caaactttctgttgtgc-----agatgatataatgcattttcactg---------------aagaaaaattctatct---tttgcagAACTTGATTTATGGTCTGGAGAAGTTCTTGTAGGATTTTATTAGAATGCTGGTAGGAATTTTCATCCCACAT--ATCAAGAGAAATACAAGCTATGGATATT

upper sequence: GRMZM2G044247_T01 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os03g02570.1 (Oryza sativa), 3'ss of exon 8
gtaacctggaactactcatgcatgggttctcttctgtgtcacagggtcgcttcgcttcactctctctggtctggtttgctgctaactaggattttgtttatttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC
                                     || |    || |  | |            |    ||| ||    ||||  |  | ||  || |||   |||||||||||||||||| || |||||||||||||||                                                                   
-------------------------------------gtaattatgttgtgtagtgcagtagatgatataatggcttttcactaaagaaaaattgtttcattc--gcagAACTTGATTTATGGTCTCGAGAAGTTCTTGTAG-------------------------------------------------------------------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148949801|gb|EC895288.2|EC895288
EST:     GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAG                         AACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
genomic: GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
EST: gi|211144011|gb|FL467385.1|FL467385
EST:     GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAG                         AACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
genomic: GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
EST: gi|18175349|gb|BM350700.1|BM350700
EST:     GACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAG                         AACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
genomic: GACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
EST: gi|76016745|gb|DT943915.1|DT943915
EST:     GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAG                         AACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
genomic: GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
EST: gi|76285578|gb|DV025146.1|DV025146
EST:     GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAG                         AACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA
genomic: GCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 3 RNA-Seq libraries.


Block sizes: 32 (upstream exon), 29 (downstream exon)
Score: 9

ATGTACACCTTACGGCATGACGTTGCGAATGTTCAGTGCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 143

ATGTACACCTTACGGCATGACGTTGCGAATGTTCAGTGCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC


Block sizes: 49 (upstream exon), 33 (downstream exon)
Score: 43

ATGTACACCTTACGGCATGACGTTGCGAATGTTCAGTGCCTGATGCCAGGACTGATCCTGGCGATACGGAAGGTGGTGCGGTTCAAGgtaacctgga ... atttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cttcactctctctggtctggtttgctgctaactaggattttgtttatttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC
                         tgctaac  putative branch site (score: 43)
                                     ttttgtttattttt  putative PPT
                                    attttgtttattttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaacctggaactactcatgcatgggttctcttctgtgtcacagggtcgcttcgcttcactctctctggtctggtttgctgctaactaggattttgtttatttttgcagAACTTGATTTATGGGCTAGAGAAGTTCTTGTAGTGAACAACAAACAACCAATGCAAAACATCGACAGGCAACAGGCAAGGCAGATATCATCTGACGTCGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGAT