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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgagcagacacagcagatacctgtaattacattcctcatagatatagcaatgttttgttctggagagagctctggtatgactgtcgctctaattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG

Basic information

species Zea mays
transcript GRMZM2G031107_T01
intron # 9
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G031107_T01 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os02g38840.1 (Oryza sativa), 3'ss of exon 9
----tgagcagacacagcagatacctgtaattacattcctcatagatatagcaatgttttgttctggagagagctctg-gtatgactgtcgctctaattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG
        ||  ||    | || |||  ||  | | |  |  |   |    || | |   |   ||  |    || |  ||||   | |||||||  | | ||||||||||||||||||||||||||||||||||||||||||||| || |||||||| ||||||||||||||||||
caacctgtcatgcattata-attcctcaaaagagaatattgctgtttc---cactttcacgacttgaggccccctttctgtattgatttcgctcttgtct-gcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAGCCTGAGCACATAAGAGATGAAAAAGTCAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|111146774|gb|EE258268.1|EE258268
EST:     TATCATTCGTGATATCATTCAAAACCACTTGTTGCAG                         GTCTTCTGTTTGGTG
genomic: TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTG
EST: gi|60672339|gb|DN475101.1|DN475101
EST:     TATCATTCGTGATATCATTCAAAACCACTTGTTGCAG                         GTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCCGAGCA
genomic: TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCC-GAGCA
EST: gi|18661166|gb|BM501251.1|BM501251
EST:     TATCATTCGTGATATCATTCAGAACCACTTGTTGCAG                         GTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCAC
genomic: TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCAC
EST: gi|76281884|gb|DV021452.1|DV021452
EST:     TATCATTCGTGATATCATTCAGAACCACTTGTTGCAG                         GTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCAC
genomic: TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCAC
EST: gi|78109019|gb|DV527436.1|DV527436
EST:     TATCATTCGTGATATCATTCAGAACCACTTGTTGCAG                         GTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCAC
genomic: TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 37 (upstream exon), 48 (downstream exon)
Score: 129

TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG


Block sizes: 37 (upstream exon), 49 (downstream exon)
Score: 530

TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG


Block sizes: 43 (upstream exon), 49 (downstream exon)
Score: 591

TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG


Block sizes: 37 (upstream exon), 48 (downstream exon)
Score: 256

TATCATTCGTGATATCATTCAGAACCACTTGTTGCAGgtaaatatat ... aattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agcaatgttttgttctggagagagctctggtatgactgtcgctctaattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG
                                         ctctaat  putative branch site (score: 256)
                                          tctaatttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgagcagacacagcagatacctgtaattacattcctcatagatatagcaatgttttgttctggagagagctctggtatgactgtcgctctaattttgcagGTCTTCTGTTTGGTGGCTATGGAAAAGCCTGTCTCTCTTAAACCCGAGCACATTAGAGATGAAAAAGTCAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATG