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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG

Basic information

species Zea mays
transcript GRMZM2G443985_T03
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os06g05860.1 (Oryza sativa), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccat-ttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
                                  ||    |  |     |    || ||||||| |    |||||  | |      |||||| | ||||      |||||    | |||||||||||||  ||||||||||||||||| ||||| |||||||| |||||||||||||||||||||||||| || |||| ||| || |||||||||||||
----------------------------------gtaatgatctttttcacattatattgacattagggtatgaattatgg--tgtatatgcat-aatttgtttctaccctatgtgcagGTAATTGACAGATCATTTGGTTTCGACACTGCAGTTGAGGAGGCCCAACGTGCAATAAATGCTGCTCATGTAGAAGCTGGAAGCGCCGAGAATGGTATAG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA07G15170.1 (Glycine max), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
||||  | ||  |      |  | ||    |||    | | |||  || |  |||||   ||| ||    |||      ||   | | |||      ||   | | |  |      |||| ||||| ||||| ||||| || ||||| || || |||||||||||||| || |||||||| || ||||| || |||| ||||| ||| ||||| ||||
gtaaggttttattttcttctacattgtggctttaaactcataatttaa-accccatt-agcatcat----tgattggtaaatttcttctgc--tgtctctttgatgcttaa----tagGTTATTGATAAGTCCTTTGGCTTTGACACTGCTGTTGAGGAGGCTCAACGAGCTATAAATGCAGCACATGTTGAAGCTGAAAGTGTTGAAAATGGCATAG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA07G01710.1 (Glycine max), 3'ss of exon 7
gtaatatgtttctccacattttagt--gacattttt-gtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtga----attcacc--gttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
          |   ||   || | |  ||||| |   |  | | ||| |||| | |  ||||     |  | |  ||  |   ||     |   || |    ||| |||  |||   | ||  |||||| ||||| |||||||||||||| ||||| || || || ||||| ||||| || |||||||||||||||||||| || | |||  ||||||||||||| |
---------gtgagcatgcttgaattggacatctgaagccttacaatagaagctac--tttgctgaaaatataaaaaggatttacttttaaactgtcacctaattaaccatgttgtacctgaatcagGTTATTGATAAGTCATTTGGTTTTGACACTGCTGTTGAAGAGGCACAACGAGCCATAAATGCTGCTCATGTGGAAGCAGAAAGCACTGAGAATGGTATTG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA08G03570.1 (Glycine max), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgat-cacatgaacaacgaaccccatatgcgtgaa------ttcaccgttacccatgtttca---gGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
                                  ||  |  |  |  ||  ||  ||  |  |  | |  ||   | |  | | ||||  || || |      || |  |||  |  ||  | |   ||| ||||| ||||| || || || |||||||| || ||||||||||||||||| |||||||| || ||||| ||||||| ||||| ||| ||||| ||||
----------------------------------gtacatcatggatagcagctcttccatttattgcttatttcccattagcaccatgagcttgtaaaatggtttaatgttctctctggcttaatagGTTATTGATAAGTCCTTCGGCTTTGACACAGCTGTTGAGGAGGCTCAACGTGCTATAAATGCAGCACATGTTGAGGCTGAAAGTGGTGAAAATGGCATAG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA04G09180.1 (Glycine max), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcacat----gaacaacgaaccccatatgcgtgaa-ttcaccgt------------tacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
    |||| || | |||| |   || | |||||||| |     |||||        ||  ||| |  | ||    |||     |   |  |||| |  || |||| | |            | |   |  || |||| |||||||| || |||||||| || || || || ||||||||||||||||| || ||||| |||||||| || |||| ||||  |||||||||||| |
---gtatgcttgttcacaat---gtcatatttttgtttctccctggaactctaatcttatcattaaaatatatctgaagtgttagttctctatgaggaaacttcatcatgttcggattgtcttctttttctttagGTTATTGACAAATCTTTTGGTTTTGATACTGCGGTTGAGGAGGCTCAACGTGCCATTAATGCAGCTCATGTTGAAGCTGAAAGTATTGAGAATGGTATTG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA13G42990.1 (Glycine max), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccat-ttgacatgatcacatgaacaacgaaccccatat----gcgtga---attcaccgttaccc-atgttt----cagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
||||  || | ||  |  || ||      | | |||| |||     |   |   ||  |  |||  ||  | | | || | |    | ||    | || |   ||| |  || |  |  | |||     |||| |||||||||||| ||||||||||||| || || || ||||| ||||| ||||| ||| ||||||||||||| || | ||| ||||||||||| || |
gtaa--tgatgcttta-gttgtaaaattgtatatgtgaaaattgttatacttttatgctatcatactccaaaggaaaaaggatttgagattaaagtgtcacctatttaatgtcattctgtatttgaattagGTTATTGACAAGTCAATTGGTTTCGACACTGCTGTTGAAGAGGCACAACGAGCAATCAATTCTGCTCATGTGGAAGCAGAAAGCGCTGAGAATGGAATTG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA01G00870.2 (Glycine max), 3'ss of exon 5
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
||||  | ||| |   | | |   |      |||      | |    | |  |||||   ||| || |    |      ||   | | |||           ||  |     |   |||| ||||| ||||| ||||| || ||||| || || |||||||||||||| || |||||||| || ||||| || |||| |||||  || ||||| ||||
gtaagttttttttttccttctacattgtggctttaaactcataatttaaaccccatt-agcatcatta----attggtaaatttcttctgc----------tgtctcttgcttaatagGTTATTGATAAGTCTTTTGGCTTTGACACTGCTGTTGAGGAGGCTCAACGAGCTATAAATGCAGCACATGTTGAAGCTGAAAGTGTAGAAAATGGCATAG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA05G36050.1 (Glycine max), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
    ||  | |||   |  |  | |  || | |         |||||      |  |||   |     |||||||    ||     | |   || ||     ||  |     |   |||| ||||| ||||| || || || |||||||| || ||||||||||||||||| |||||||| || ||||| ||||||| ||||| ||| ||||| ||||
---gtacatctcttttctgtccattatcaatatc--------atggatagcagctcttgcattagcaccatgaacttgtaaaatggttta-atgcat--gttgtctctggcttaatagGTTATTGATAAGTCCTTCGGCTTTGACACAGCTGTTGAGGAGGCTCAACGTGCTATAAATGCAGCACATGTTGAGGCTGAAAGTGGTGAAAATGGCATAG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA08G21370.1 (Glycine max), 3'ss of exon 7
gtaatatgtttctccacattttagtgacattttt-gtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtt---------tcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
    || |  |      |||     |||||     |  | | ||| |||| | |  |||||   ||    |  ||||||||    | | |         |    ||| |||||||         |||||| || || |||||||| ||||| ||||| || || || ||||| ||||| || |||||||||||||||||||| || | |||  ||||||||||||| |
---gtaagcatgcttgaattg----gacatccgaagccttacaatagaagctac--tttgatgagaatgtaaaaacaacgatatacttttaaacagtcacctaattaaccatgttgtacctgaatcagGTTATCGATAAGTCATTCGGTTTTGACACTGCTGTTGAAGAGGCACAACGAGCCATAAATGCTGCTCATGTGGAAGCAGAAAGCACTGAGAATGGTATCG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA15G02400.1 (Glycine max), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaag-----atgccatttgacatgatcacatgaacaa----cgaaccccatatg-cgtgaattcaccgttaccca-----tgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
||||  || | ||  |  || ||      | | |||| |||     |       |||||||   ||  ||    |  || |      ||     |  || |    ||| |  || |  |      || || |||| |||||||| ||| ||||||||||||| || || || ||||| ||||| ||||| ||| ||||||||||||| || | ||| || |||||||| || |
gtaa--tgatgcttta-gttgtaaaattgtatatgtggaaattgctatacttttatgccatcgtactcgaaaggaaaaaaagaatttgagtttaaagtgtcacctatttaatgtcattctgtatttgatttagGTTATTGACAAATCAATTGGTTTCGACACTGCTGTTGAAGAGGCACAACGAGCAATCAATTCTGCTCATGTGGAAGCAGAAAGCGCGGAGAATGGAATTG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA06G09320.1 (Glycine max), 3'ss of exon 7
---------------gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcac-atgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
                |   || ||| |       |   ||  | | |  | | |  ||  || || | ||||||  || |    ||  |  | ||| |    |||   | |||  |   | |   |  || |||| |||||||| || |||||||| || || || || ||||||||||||||||| || ||||| |||||||| || |||| ||||  ||||||||| || |
gtatgcttgttcacaatgtcatatttttt-gttcctccctggaactctaatcttatcatt-aaaatac-atttgaagtgttggttatctatgaagaaactt-catgttcggattgcc---ttctttttctttagGTTATTGACAAATCTTTTGGTTTTGATACTGCGGTTGAGGAGGCTCAACGTGCCATTAATGCAGCTCATGTTGAAGCTGAAAGTATTGAGAATGGCATTG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: AT4G32840.1 (Arabidopsis thaliana), 3'ss of exon 8
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
||||    |   |   |||| | ||     |    | | ||  |  |      |   |    || | |  |   |||  | | | || ||  |||         || |  || |  |||| ||||||||||| ||||| || || || || || ||||||||||||||||| || ||||| || ||||| || ||    ||||  || || || || |
gtaagtcctagattatcattattgtagt-ctgcactctcaaccttaacctcctctcctattctggtta-gttcgcaagaagctcaatttg-atga------tagtatctctgatctagGTCATTGACAAGTCCTTTGGATTTGATACTGCGGTTGAGGAGGCTCAACGTGCTATTAATGCAGCGCATGTTGAAGCGACCAGTGTGGAAAACGGCATTG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: Vv11s0016g02850.t01 (Vitis vinifera), 3'ss of exon 8
----------------------------gtaatatgttt--ctccacattttagtgacatttttgtgtaaaaatgga---agatgccatttgacatgatcacatgaacaacgaaccccatatgc-gtgaattc--accgttacccat----gtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
                             | ||| |      |  | |||  |      || |||  || | ||  |   | |  | ||||            | |   || ||  |  | |   ||  |||   |||     | ||    | | ||||| ||||||||||| ||||| || ||||| || || |||||||| |||||||| || ||||| || ||||| ||  ||| ||||   |||||| |||| |
gtacatctctttgtttccttaattgctgattataggaccggttggatattacacctgatttattgcatagatataaattcaaaaacaatttttttcctattttttattgaccaattctctttttagttgatttttacctccgactattgctggtacagGTTATTGACAAGTCCTTTGGCTTTGACACTGCTGTTGAGGAGGCCCAACGTGCTATTAATGCAGCACATGTTGAATCTGAAAGTATCGAGAATTGTATTG

upper sequence: GRMZM2G443985_T03 (Zea mays), 3'ss of exon 8
lower sequence: PP1S131_40V6.1 (Physcomitrella patens), 3'ss of exon 8
--------------------------------------------------gtaa-tatgtttct-----ccacattttagtgacatttttgtgtaaaaa-tggaagatgccat--ttgaca------tgatcacatgaacaacgaa-----ccccatatg-cgtgaattcaccgttacccatgtttcagGTAA-------TTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG
                                                  |||| || |||||      | |   ||| |  || | | || | ||| | |   ||||| |||  || |        || |  | ||||   | |      | || | |   |   |||   ||       | ||| ||||||       | || |||||||| || || || |||||||| || || ||||| |||||||| ||||| ||||||||||| ||||  |||| | |||||||| | |
gtacgtcactgtcttttgatatacacttggtaggattacgtgcggtagaagtaactaagtttcaggaagctaatatttggatacgtgtgtgcgcaaagactaccagatgacatgattaattatgtattgttttcttgaagtccaagtgtgccaccgtctaatgcatatttgtcgaatgtggtatttgaggtaacagGTCATAGATAAGTCATTCGGATTTGATACAGCAGTGGAAGAAGCTCAGCGTGCAATCAATGCAGCTCATGTGGAAGCTGAGAGTGTTCAGAATGGTCTTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32938375|gb|CF043194.1|CF043194
EST:     TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACGTCCCT                         GTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
genomic: TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCTgtaatatgtt ... catgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
EST: gi|32938968|gb|CF043787.1|CF043787
EST:     TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCT                         GTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
genomic: TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCTgtaatatgtt ... catgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
EST: gi|32939806|gb|CF044625.1|CF044625
EST:     TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCT                         GTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
genomic: TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCTgtaatatgtt ... catgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
EST: gi|32939493|gb|CF044312.1|CF044312
EST:     TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCT                         GTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
genomic: TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCTgtaatatgtt ... catgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
EST: gi|13557855|gb|BG549211.1|BG549211
EST:     TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCT                         GTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
genomic: TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCTgtaatatgtt ... catgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
EST: gi|76921243|gb|DV168509.1|DV168509
EST:     TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCT                         GTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
genomic: TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCTgtaatatgtt ... catgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
EST: gi|211132969|gb|FL170874.1|FL170874
EST:     TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCT                         GTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT
genomic: TCAAAGTTTCTGTTGCTGGCATTCCAAAGACTATTGACAATGACATCCCTgtaatatgtt ... catgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGT

Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaatatgtttctccacattttagtgacatttttgtgtaaaaatggaagatgccatttgacatgatcacatgaacaacgaaccccatatgcgtgaattcaccgttacccatgtttcagGTAATTGACAAGTCATTTGGTTTCGACACAGCAGTCGAGGAGGCTCAACGTGCAATAAATGCTGCTCATGTGGAGGCTGTAAGTGCTGAGAATGGTATAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ATGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGG