ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtgaggatccatattttgttttggaatcccattcgggagcgggaccaaactgaccttaacgcaatgtgcctctcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCTCCGGCTCACATCCTCAGCAGAGGCATTGGGTGGCAG

Basic information

species	Zea mays
transcript	GRMZM2G437859_T04
intron #	8
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G437859_T02 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os01g55500.4 (Oryza sativa), 3'ss of exon 8

gtgaggatcc--atattttgttttggaatcccattcgggagcgggaccaaactgaccttaacgcaatgtgcctctcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCTCCGGCTCACATCCTCAGCAGAGGCATTGGGTGGCAG
|| | |  ||  |||  | || ||      |||||     ||| | |     ||| |||||| ||| |  ||| ||||||||||||||| || || |||||||| || ||||| ||||| ||||| ||||||||||| || || |||||||| ||||||||||||||||||
gtaatgcccctcataactcgtcttcaggagccatttctttgcgtggcagccttgaacttaactcaaag--cctgtcttggacagTCCACGGCTTCTTACAAAGCTGCGGCTCGGCTTGCAAGTGCTACTCCACCTCCAGCACATATCCTCAGTAGAGGCATTGGGTGGCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|74238881|gb|DT646795.1|DT646795

EST:     CCGGCCAGTCATTTGGGATTGTGTCTGCTGTTTTGGTCTCGCTGGTAGAG                         TCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT
genomic: CCGGCCAGTCATTTGGGATTGTGTCTGCTGTTTTGGTCTCGCTGGTAGAGgtgaggatcc ... tcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT

EST: gi|50337438|gb|CO532564.1|CO532564

EST:     GCTGTTTTGGTCTCGCTGGTAGAG                         TCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT
genomic: GCTGTTTTGGTCTCGCTGGTAGAGgtgaggatcc ... tcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT

EST: gi|211379070|gb|FL028411.1|FL028411

EST:     CCGGCCAGTCATTTGGGATTGTGTCTGCTGTTTTGGTCTCGCTGGTAGAG                         TCCACAGCCTCCTACAAAGCCGCTGCTCGGCTTGCGAGTGCGACTCCACCT
genomic: CCGGCCAGTCATTTGGGATTGTGTCTGCTGTTTTGGTCTCGCTGGTAGAGgtgaggatcc ... tcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT

EST: gi|5055765|gb|AI734652.1|AI734652

EST:     CCGGCCAGTCATTTGGGATTGTGTCTGCTGTTTTGGTCTCGCTGGTAGAG                         TCCACAGCCTCCTACAAAGCCGCTGCTCGGCTTGCGAGTGCGACTCCACCT
genomic: CCGGCCAGTCATTTGGGATTGTGTCTGCTGTTTTGGTCTCGCTGGTAGAGgtgaggatcc ... tcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT

EST: gi|76287139|gb|DV026707.1|DV026707

EST:     GCTGTTTTGGTCTCGCTGGTAGAG                         TCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT
genomic: GCTGTTTTGGTCTCGCTGGTAGAGgtgaggatcc ... tcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 209

GATTAAGATCCCCTACCCACTGCAATGGGGTGCGCCGACCTTCAACGCCGGCCAGTCATTTGGGATTGTGTCTGCTGTTTTGGTCTCGCTGGTAGAGgtgaggatcc ... tcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCTCCGGCTCACATCCTCAGCAGAGGCATTGGGTGGCAG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 43

Block sizes: 38 (upstream exon), 49 (downstream exon)
Score: 25

Block sizes: 40 (upstream exon), 47 (downstream exon)
Score: 19

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

cccattcgggagcgggaccaaactgaccttaacgcaatgtgcctctcttggacagTCCACAGCCTCCTACAAAGCCGCTGCTCGTCTTGCGAGTGCGACTCCACCTCCGGCTCACATCCTCAGCAGAGGCATTGGGTGGCAG