ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtttgtttacacttcttgatgacctcacctttgctagaagagttttgaacaattgaactgcattttgttatgacttgtgatagttgtttttgtttatatgatttgcatcttatgctctacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGACAAAGATTTTGTTGAGGGTCCTTGGTCCCAGAATAATGTAGATAATGGTG

Basic information

species	Zea mays
transcript	GRMZM2G402002_T01
intron #	8
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G402002_T01 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os05g51480.1 (Oryza sativa), 3'ss of exon 8

gtttgtttacacttcttgatgacctcacctttgctagaagagttt--tgaacaattgaactgcattttgttatgacttgtgatagttgtttttgtttatatgatttgcatcttatgctctacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGACAAAGATTTTGTTGAGGGTCCTTGGTCCCAGAATAATGTAGATAATGGTG
                   | || |    |||  |  ||   | |  ||       || ||  ||||    ||    || ||||||           || ||||||   |   ||||| |||    |||||||| |||||||  || ||||||||||| |||||||||||| ||||||||||||| |||||||||||||||||||| ||||| ||| |||| |||||||
-----------------atttacttttgatttattcaaaatatctgctggttctcagatctttatttgaccattg--tgagatagtaaaag------atctgatttaatttgcatgctttac----cagGATAACAAAGATGCAAGACATGTTAAGACATATGAAGTTGCATTGAAGGACAAAGACTTTGTTGAGGGTCCTTGGTCGCAGAACAATTTAGACAATGGTG

upper sequence: GRMZM2G402002_T01 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA05G30530.1 (Glycine max), 3'ss of exon 8

gtttgtttacacttc-ttgatgacctcacctttgctagaagagttttgaacaattgaactgcattttgttatgacttgtgatagttgtttttgtttatatgatttgcatcttatgctctacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGACAAAGATTTTGTTGAGGGTCCTTGGTCCCAGAATAATGTAGATAATGGTG
      || || ||| || |||   |    |||| | ||| | |  ||   |    ||   || |||    ||| || |||| | |    |||||  |||| ||| ||||           |   ||||||||||||||||   | |||||||| || ||||| |||||||||||||| ||||||||| |||| ||||| ||||||||||| ||| | |||||||| |
------ttgcatttcctttatgcaatatattttgttggaaaaatggtgtttagcataa--acaattt----tgattt-tgatggat----ttgttattatg-ttttcatcg----------atgacagGATAATAAAGATGCTCGTCATGTTAAAACATATGAGGTTGCACTGAAGGATAAAGATTTTCTTGAAGGTCCATGGTCCCAGAACAATCTTGATAATGGGG

upper sequence: GRMZM2G402002_T01 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA08G13680.1 (Glycine max), 3'ss of exon 8

gtttgtttacacttcttgatgacctcacctttgctagaagagttttgaacaattgaactgcattttgttatgacttgtgatagttgtttttgtttatatgatttgcatcttatgctctacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGACAAAGATTTTGTTGAGGGTCCTTGGTCCCAGAATAATGTAGATAATGGTG
                   |  | |  |||||  |  || |  ||||        ||  |  ||| |     || | |     ||| ||||| |    |  ||   || || |  || |     ||||||||||||||||   | |||||||| || ||||| |||||||||||||| |||||||||||||| ||||| ||||||||||| ||| | |||||||| |
-------------------ttgcatttccttta-tgcaatatatttggttggaaaaatggtgtttagcataaacattt-----ttgattttgatggatttgttactatgttttcatcaatg--acagGATAATAAAGATGCTCGTCATGTTAAAACATATGAGGTTGCACTGAAGGATAAAGATTTTGTTGAAGGTCCATGGTCCCAGAACAATCTCGATAATGGGG

upper sequence: GRMZM2G402002_T01 (Zea mays), 3'ss of exon 8
lower sequence: Vv10s0003g04360.t01 (Vitis vinifera), 3'ss of exon 8

gtttgtttacacttcttgatgacctcacctttgctagaagagttttgaacaattgaactgcattttgttatgacttgtgatagttgtttttgtttatatgatttgcatcttatgctctacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGACAAAGATTTTGTTGAGGGTCCTTGGTCCCAGAATAATGTAGATAATGGTG
                          | ||| | | |      | ||  | |||     ||||   |   ||  ||  ||    |   |  | |    | |      |||  || |  | | | |||||||| |||||||   | |||||||| || ||||| || ||  ||||||| || ||||||||||||||||||||| | ||||| ||| |||| ||||| |
--------------------------aacttagatggccccactgtgttccattatgtagcatca-gaagtgcattaggaagaataactcagatggcctcaccatgttctcttgattcaaattgcagGATAACAAAGATGCACGTCATGTTAAAACATATGAGGTGGCTTTGAAGGATAAGGATTTTGTTGAGGGTCCTTGGGCTCAGAACAATCTAGACAATGGGG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|74245573|gb|DT653487.1|DT653487

EST:     TCAAATTTCTACATGGTTGTGCTAAACCTACTATTGTTGTCCTCTACCAG                         GATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGAC
genomic: TCAAATTTCTACATGGTTGTGCTAAACCTACTATTGTTGTCCTCTACCAGgtttgtttac ... tacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGAC

EST: gi|32943509|gb|CF048328.1|CF048328

EST:     TCAAATTTCTACATGGTTGTGCTAAACCTACTATTGTTGTCCTCTACCAG                         GATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGAC
genomic: TCAAATTTCTACATGGTTGTGCTAAACCTACTATTGTTGTCCTCTACCAGgtttgtttac ... tacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGAC

EST: gi|32933109|gb|CF037921.1|CF037921

EST:     TCAAATTTCTACATGGTTGTGCTAAACCTACTATTGTTGTCCTCTACCAG                         GATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGAC
genomic: TCAAATTTCTACATGGTTGTGCTAAACCTACTATTGTTGTCCTCTACCAGgtttgtttac ... tacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGAC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 353

ACTTGAAGAACTTCAAGTACTGGACATCAAATTTCTACATGGTTGTGCTAAACCTACTATTGTTGTCCTCTACCAGgtttgtttac ... tacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGACAAAGATTTTGTTGAGGGTCCTTGGTCCCAGAATAATGTAGATAATGGTG

Block sizes: 48 (upstream exon), 37 (downstream exon)
Score: 116

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 110

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 152

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

acttgtgatagttgtttttgtttatatgatttgcatcttatgctctacacttcagGATAATAAAGATGTGAGGCATGTTAAGACTTATGAAGTTGCACTGAAGGACAAAGATTTTGTTGAGGGTCCTTGGTCCCAGAATAATGTAGATAATGGTG