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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ctattttccccatgcgcagtggggagccgctggggctagccttactaacagtctcttattgctacatgaatactgacatttttttccaattaacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACTGCCCAGTT

Basic information

species Zea mays
transcript GRMZM2G168510_T02
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G168510_T02 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os12g10784.1 (Oryza sativa), 3'ss of exon 7
ctattttccccatgcgcagtggggagccgctggggctagccttactaaca----gtctcttattgctacatgaatactgacatttttttccaattaacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACTGCCCAGTT
  ||| ||    |    | |    |     ||   |    |  |  || |    || | | |||||| |||||    ||| ||| | ||   |||   |||||||||||||| ||||||| ||||||||  ||||||||||||| |||   || || ||||||
-cattgtctgtttat-taatatttattt-ttgcaccctctccaaagaaaattatgtttttgattgcttcatgacggttga-attctgttttcattccgacacagATGATCTAATTGTTGATCCAAGGAATTTTGAGTGGCTGAGAGAAGCTAATTGTCCAGTT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|5847371|gb|AW000450.1|AW000450
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|60351240|gb|DN218213.1|DN218213
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|4887386|gb|AI677485.1|AI677485
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|61118196|gb|DN559157.1|DN559157
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|5296161|gb|AI783036.1|AI783036
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|60340487|gb|DN207460.1|DN207460
EST:     CGTGGTTACCATGTTTGGCTACA                         ATGATCTATTTG-TGACCCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAAC
genomic: CGTGG-TACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGA-CCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAAC
EST: gi|91055907|gb|EB166325.1|EB166325
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|50323772|gb|CO518898.1|CO518898
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|50323794|gb|CO518920.1|CO518920
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|71307284|gb|DR789817.1|DR789817
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|5871261|gb|AW017732.1|AW017732
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|9794838|gb|BE553146.1|BE553146
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGGTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAG
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAG
EST: gi|60400102|gb|DN232909.1|DN232909
EST:     CAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: CAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|74236360|gb|DT644274.1|DT644274
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|60396848|gb|DN229670.1|DN229670
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|6227240|gb|AW155839.1|AW155839
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|60398159|gb|DN230975.1|DN230975
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|5871277|gb|AW017748.1|AW017748
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|4887387|gb|AI677486.1|AI677486
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTCCCATGTTTGGCTACA                         ATGATCTCTTTGTTGACCCAAGGAACCTTGAGTGCCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|61118194|gb|DN559155.1|DN559155
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|74236361|gb|DT644275.1|DT644275
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAACAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|5770206|gb|AI973380.1|AI973380
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|91050684|gb|EB161102.1|EB161102
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTT
EST: gi|31362214|gb|CD446571.1|CD446571
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|60357398|gb|DN224371.1|DN224371
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|5847238|gb|AW000317.1|AW000317
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|60359317|gb|DN226290.1|DN226290
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|78022942|gb|DV491329.1|DV491329
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|71763878|gb|DR961815.1|DR961815
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|31355531|gb|CD439888.1|CD439888
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|211382425|gb|FK948866.1|FK948866
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTT
EST: gi|60355225|gb|DN222198.1|DN222198
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|6828178|gb|AW331821.1|AW331821
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|71449637|gb|DR830687.1|DR830687
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|211051797|gb|FL187615.1|FL187615
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|91051935|gb|EB162353.1|EB162353
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|33101621|gb|CF061581.1|CF061581
EST:     GGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: GGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|61118195|gb|DN559156.1|DN559156
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|31361455|gb|CD445812.1|CD445812
EST:     TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: TGGGAATCCAAATGTGATGGTCTGTGAGCGTGGTACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
EST: gi|4730747|gb|AI649913.1|AI649913
EST:     ACCATGTTTGGCTACA                         ATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT
genomic: ACCATGTTTGGCTACAgttaaggcat ... taacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACT


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

taacagtctcttattgctacatgaatactgacatttttttccaattaacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACTGCCCAGTT
                                atttttttccaattaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ctattttccccatgcgcagtggggagccgctggggctagccttactaacagtctcttattgctacatgaatactgacatttttttccaattaacacacagATGATCTATTTGTTGACCCAAGGAACCTTGAGTGGCTGAGGGAAAGCAACTGCCCAGTT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCAA
- - - - - catgcgc
- - - - - - - - - - - - -gccgctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ctacatg