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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatgcgtgctttatgtcctataacaaacaaatagaaactattatttcgtaaccagttactgatcttatttacactattatactgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA

Basic information

species Zea mays
transcript GRMZM2G168412_T01
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G168412_T01 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os01g74190.1 (Oryza sativa), 3'ss of exon 9
gtatgcgtgctttatgtcctataacaaacaaatagaaactattatttcgtaaccagttactg-atcttatttacactat-tatactgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA
|||||| ||   |||  | ||||  |  ||  ||| ||  | |  |||      || | ||| || ||||  | |  ||   |  |    |  |||| ||||| |||||||||||  ||||||| || ||||||||||| ||||||||||  |||||||||||||| ||||| || || || || || || ||||
gtatgcttg---tatcgcttata-tatgcat-tagtaaggagttgttcaatttgagctgctgtattttatgcatatgatacctgatatggac-agGTTCAAGCGTTTGTTGATGCGCTAGAAGCTCGGAAGATAACCGTAAGCGTTCGACAAACCCGTGGGCTTGATGCTAATGCCGCTTGCGGACAACTAAGGA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|91878765|gb|EB408722.1|EB408722
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|71766718|gb|DR964655.1|DR964655
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|88747606|gb|DY531747.1|DY531747
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|13149611|gb|BG319933.1|BG319933
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|32940242|gb|CF045061.1|CF045061
EST:     ACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: ACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|78122643|gb|DV541027.1|DV541027
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|60345957|gb|DN212930.1|DN212930
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|71328602|gb|DR800966.1|DR800966
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|78118843|gb|DV537227.1|DV537227
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|32938957|gb|CF043776.1|CF043776
EST:     ACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: ACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|76286139|gb|DV025707.1|DV025707
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|32940213|gb|CF045032.1|CF045032
EST:     ACAAAAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: ACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|76015835|gb|DT943005.1|DT943005
EST:     CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
EST: gi|78093019|gb|DV521393.1|DV521393
EST:     CCTATAACCCAATTGAAGGCTCGGAGTACAAGAGACCTTACAGAAAAGTG                         GTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT
genomic: CCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 43 (downstream exon)
Score: 47

AGCAGAACTACTTCGTATGTGTGGAGGTGGTTACCATGTGAACCTGATTCCCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 134

AGCAGAACTACTTCGTATGTGTGGAGGTGGTTACCATGTGAACCTGATTCCCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 45

AGCAGAACTACTTCGTATGTGTGGAGGTGGTTACCATGTGAACCTGATTCCCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 52

AGCAGAACTACTTCGTATGTGTGGAGGTGGTTACCATGTGAACCTGATTCCCTATAACCCAATTGAAGGCTCCGAGTACAAGAGACCTTACAGAAAAGTGgtatgcgtgc ... tgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ctattatttcgtaaccagttactgatcttatttacactattatactgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA
                   tactgat  putative branch site (score: 52)
                           ttatttacactattat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatgcgtgctttatgtcctataacaaacaaatagaaactattatttcgtaaccagttactgatcttatttacactattatactgcaaattagGTCCAAGCCTTTGTTGATGCTTTAGAAGCACGCAAGATAACCGTTAGCGTTCGACGGACCCGTGGGCTTGACGCTAACGCAGCCTGTGGGCAGCTGAGGA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGA